| Project/Area Number |
08555202
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
OKAHATA Yoshio Tokyo Institute of Technology, Department of Biomolecular Engineering, Professor, 生命理工学部, 教授 (80038017)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Yukio AmasiamPharmasia Co., Director of Researches, 研究開発部, 部長
UESHIMA Takayuki NovoNordisuku Industries, Director of Researches, イスクバイオインダストリー(株)・研究開発部, 主任研究員
MORI Toshiaki Tokyo Institute of Technology, Department of Biomolecular Engineering Research A, 生命理工学部, 助手 (50262308)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1997: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1996: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Lipid-coated enzyme / Enzyme reaction in Organic Media / Enantioselective reactions / Lipase / Esterification |
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| Research Abstract |
We have prepared a lipid-coated enzyme, in which enzyme surfaces were covered with a monolayer of dialkyl amphiphiles and alkyl chains could solubilize enzymes in organic media. The lipid-coated enzymes can be solubilized in most of organic solution and act as an efficient catalyst. Following results were obtained.
1) A lipid-coated enzyme was obtained by mixing both aqueous solutions of enzyme and amphiphiles, and obtained precipitated are soluble only in organic media, but not in aqueous solutions.
2) The lipid-coated lipase can catalyze the enantioselective esterification (reverse reaction of hydrolysis) in isooctane. When the lipid-coated galactosidase was prepared, it acts as an efficient catalyst as transglycosylation in isopropyl ether.
3)We believe the lipid-coating system can be applied for most of enzymes to solubilize them in organic media without denaturation.
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