| Project/Area Number |
08555204
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University |
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Principal Investigator |
KOBAYASHI Akio Osaka University, Department of Engineering, Professor, 大学院・工学研究科, 教授 (30115844)
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| Co-Investigator(Kenkyū-buntansha) |
SENDA Shuji Nitto Denko LTD., Medical department, Chief investigator, メディカル事業部, 主任研究員
KAJIYAMA Shin'ichiro Osaka University Department of Engineering, Assistant Professor, 大学院・工学研究科, 助手 (20243496)
FUKUSAKI Eiichiro Osaka University Department of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (40273594)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1996: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | Enzymatic reaction / Peroxidase / Laccase / Antibiotics / Adhesive polymer / Detergent / Isoeugenol / ペルオキシダーゼ / 一電子酸化 / ラジカル / ヒドロキシスチルベン / パーオキシダーゼ / 抗菌性 / 重合反応 / グアイアコール / 金属イオン |
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| Research Abstract |
At first, we paid an attention to high radical oxidizing activity of peroxidase (POD) from plant to synthesize various antimicrobial compounds. We succeeded to prepare several antimicrobial compounds derived from several phenolics such as guaicol, isoeugenol, ferulic acid, caffeic acid, thymol, carvacrol and hydroxystilben via POD coupling reaction. Among them, we chose a POD reaction product derived from isoeugenol as an antimicrobial compound for further experiments. Next, we prepare several prototypes of adhesive polymer for surgical tapes. We used following polymers such as HPAO, PANTA, PAOB, and NITTO for base adhesive polymer. POD reaction products were mixed above base polymer in toluene to be yielded dope mixture for coating onto the polymer supports. After coating, the preparations were dried up by the drying oven to remove solvent. We examined effects of drug concentrations on their antimicrobial activities and clarified that more than 10% of drug content is essential for expression of antimicrobial activity. Actually, adhesive polymer containing 20% of active drug from isoeugenol show an apparent antimicrobial activity against Staphyrococuss aureus. We suggested that hydrophobicity of isoeugenol POD products cause its low permeability to medium and studied effects of surfactant addition into adhesive polymer. However, addition of surfactant such as Tween 20 or Tween 8O caused aggregation of polymer. We guess that above phenomena was caused from reaction between nonionic surfactant and aromatic multivalent isocyanic crosslinker. In conclusion, PAOB and PANTA were good candidates as base polymers for this study. Further investigation about crosslinker should be performed.
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