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Development of diagnostic probes allowing the identification of male-sterile and male-fertile cytoplasms in beets

Research Project

Project/Area Number 08556001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Breeding science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MIKAMI Tetsuo  Fac.of Agr., Hokkaido University, Pro., 農学部, 教授 (50133715)

Co-Investigator(Kenkyū-buntansha) KOIKE Takayoshi  Fac.of Agr., Hokkaido University, Prof., 農学部, 教授 (10270919)
MAEKAWA Masahiko  Res.Inst.for Bio., Okayama University, Asso.Prof., 資源生物科学研究所, 助教授 (00142703)
IKEGUCHI Shojiro  Hokuren Agr., Res.Inst., Res Agro., 植物工学科, 副専門研究員
KUBO Tomohiko  Fac.of Agr., Hokkaido University, Inst., 農学部, 助手 (40261333)
SANO Yoshio  Fac.of Agr., Hokkaido University, Pro., 農学部, 教授 (70109528)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥17,800,000 (Direct Cost: ¥17,800,000)
Fiscal Year 1998: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1997: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1996: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordssugarbeet / wild beet / cytoplamic male sterility / mitochondrial DNA / diagnostic probes / Southern blot analysis / genetic vulnerability / cytoplasmic diversity / Beta maritima / ミトコンドリアゲノム / マスターサークル / サブゲノム分子 / ゲノム再編成 / モザイク構造 / diagnosticプローブ系 / 細胞質雄性不念性 / フダンソウ / クローンライブラリー / RELP / diagnosticプローブ糸 / ハイブリッド育種 / RFLP
Research Abstract

All current sugarbeet cultivars are based on the only one type (the Owen type) of male-sterile cytoplasm. This cytoplasmic uniformity renders the crop vulnerable to cytoplasm-specific diseases, and a broadening of the cytoplasrnic variation is desirable. The objective of this study was to screen Beta vulgaris and B.maritima germplasm accessions for differences in their mtDNAs, using the diagnostic probes to identify new male-sterile cytoplasm's.
We developed 31 probes prepared from a mitochondrial DNA clone bank of sugarbeet. These probes were sequentially used for Southern blot analysis of total DNA from the germplasm accessions. The results indicated that most of the wild beet accessions have novel types of rnitochondrial genomes and that only few are completely identical to previously known genomes such as the Owen type and normal fertile genomes. The novel mitochondrial genome type can be classified into two categories, the first one being similar to known genomes, and the second representing a mosaic organization of genome. We also found that several wild beets possessing the novel mitochondrial genomes show pollen sterility. It is thus of interest to determine whether or not the novel mitochondrial genome types detected here are correlated with cytoplasmc male sterility.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] A.Estiati: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantarum. 102. 325-327 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Senda: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. 128. 127-132 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Senda: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. 96. 964-968 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Koike: "Mitochondrial DNA variation follows a geographic pattern in Japanese beech species." Botanica Acta. 111. 87-91 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kishima: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose." Plant Molecular Biology. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Onodera: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet." Journal of Plant Physiology. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Estiati, T.Kubo and T.Mikami: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantarum. 102. 325-327 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Senda, Y.Onodera and T.Mikami: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. 128. 127-132 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Senda, Y.Onodera and T.Mikami: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. 96. 964-968 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Koike, S.Kato, Y.Shimamoto, K.Kitamura, S.Kawano, K.Ueda and T.Mikami: "Mitochondrial DNA variation follows a geographic pattern in Japanese beechspecies." Botanica Acta. 111. 87-91 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Kishima, S.Yamashita, C.Martin and T.Mikami: "Structural conservation of the transposon Tam3 family in Antirrhinum majusand estimation of the number of copies able to transpose." Plant Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Onodera, M-P.Yamamoto, T.Kubo and T.Mikami: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet." Journal of Plant Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Estiati: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantarum. 102. 325-327 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Senda: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. 128. 127-132 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Senda: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. 96. 964-968 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Koike: "Mitochondrial DNA variation follows a geographic pattern in Japanese beech species." Botanica Acta. 111. 87-91 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Kishima: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose." Plant Molecular Biology. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Onodera: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet." Journal of Plant Physiology. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Aii J.: "Expansion of the IR in the chloroplast genomes of buckwheat species is due to incorporation of an SSC sequence that could be mediated by an inversion" Current Genetics. 31. 276-279 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Senda M.: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets" Theoretical and Applied Genetics. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Estiati A.: "The ribosomal protein S7 gene is transcribed and edited in sugarbeet mitochondria" Physiologia Plantarum. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Senda M.: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis" Hereditas. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Koike T.: "Mitochondrial DNA variation follows a geographic pattern in Japanese beech species" Botanica Acta. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Tozuka A.: "Composite and clinal distribution of Glysine soja in Japan revealed by RFLP analysis of mitochondrial DNA" Theoretical and Applied Genetics. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 山田康乃編: "植物分子生物学" 朝倉書店, 195 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubo, T.: "A duplicated sequence in sugarbeet mitochondrial trans cripts is differentially edited : analysis of orfB and its derivative orf324 mRNAs." Biochimica et Biophysica Acta. 1307. 259-262 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aii, J.: "Expansion of the IR in the chloroplast genomes of buckwheat species is due to incorporation of an SSC sequence that could be mediated by an inversion." Current Genetics. (in press)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三上 哲夫: "細胞質雄性不稔性-発現のメカニズムと雄性不稔遺伝子の設計" 遺伝. 50・61. 38-43 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y, Sano: "Genetic dissection of cross incompatibility in rice." Rice Genetics. III. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maekawa, M.: "Genic analysis of hybrid sterility caused by anther indehiscency between distantly related rice varieties." Euphytica. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Maekawa, M.: "Trapping of high cool-tolerant gene (s) at booting stage from rice germplasm." (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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