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Development of Rapid Rvaluation Methods for Immunopotentiators of Fish.

Research Project

Project/Area Number 08556032
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General fisheries
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YANO Tomoki  Kyushu University, Faculty of Agriculture, Professor, 農学部, 教授 (90038266)

Co-Investigator(Kenkyū-buntansha) NAKAO Miki  Kyushu University, Faculty of Agriculture, Assistant Professor, 農学部, 助手 (50212080)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsFISH / IMMUNOPOTENTIATOR / COMPLEMENT / COMPLEMENT RECEPTOR / beta-1,3-GLUCAN / SODIUM ALGINATE / CYTOKINES / コイ / 免疫学 / 補体レセプター / モノクローナル抗体
Research Abstract

To develop rapid evaluation methods of immunopotentiators in fish, the mechanism of the immunopotentiation in carp was analyzed using a fungi-derived beta-1,3-glucan and sodium alginate prepared from a brown algae as model materials of immunopotentiators. The beta-1,3-glucan activated the complement of carp and intraperitoneal administration of the beta-1,3-glucan elevated the expression level of mammalian CR3-like complement receptors on head kidney phagocytes. Therefore, the enhancement of phagocytic activity of the phagocytes which is endowed by the increase in complement receptors expression probably plays an important role in elevation of host defense against bacterial infection. On the basis of these findings, we have isolated carp C3a fragment, which would be a good indicator for complement activation, and developed the a detection method of carp C3a as well as a rosette assay of the receptors. On the other hand, neither activation of complement nor enhancement of phagocyte activity was observed after intraperitoneally injected sodium alginate. Rather, sodium alginate induced a great migration of head kidney leukocytes to the peritoneal cavity, indicating that sodium alginate stimulate the release of chemotactic cytokines from resident leukocytes in the cavity. These results show that there are distinct mechanisms underlying the enhancing effects of those polysaccharide immunopotentiators on the fish host defense system. Functional and immunochemical assays of cytokines are not applicable to fish, because few data has been accumulated on fish cytokines. However, analysis of gene-expression, which is specifically stimulated by the administration of immunopotentiator, will help to overcome the difficulties.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Fujiki: "Effects of sodium alqinate on the non-specific defence system of the common carp(Cyprinus carpio L.)" Fish and Shellfish Immunology. 7-6. 417-427 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fujiki: "Effects of k-carrageenan on the non-specific defense system of carp(Cyprinus carpio)" Fisheries Science. 63-6. 934-938 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fujiki: "Protective effect of k-carrageenan against bacterial infections in carp Cyprinus carpio" J.Fac.Agr.,Kyushu Univ.42-1,2. 113-119 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujiki, K.and Yano, T.: "Effects of sodium alginate on the non-specific defense system of he common carp(Cyprinus carpio L.)." Fish and Shellfish Immunology. 7(6). 417-427 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujiki, K., Shin, D.H., Nakao, M., and Yano, T.: "Effects of kappa-carrageenan on the non-specific defense system of carp(Cyprinus carpio)." Fisheries Sci.63(6). 934-938 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujiki, K., Shin, D.H., Nakao, M., and Yano, T.: "Protective effects of kappa-carrageenan against bacterial infections in carp Cyprinus carpio." J.Fac. Agr., Kyushu Univ.42(1/2). 113-119 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fujiki: "Effects of sodium alginate on the non-specific defence system of the common carp(Cyprinus carpio L.)" Fish and Shellfish Immunology. 7-6. 417-427 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fujiki: "Effects of k-carrageenan on the non-specific defense system of carp(Cyprinus carpio)" Fisheries Science. 63-6. 934-938 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Fujiki: "Protective effect of k-carrageenan against bacterial infections in carp Cyprinus carpio" J.Fac.Agr.,Kyushu Univ.42-1,2. 113-119 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshi Uemura: "Purification and characterization of the eighth and ninth components of carp complement" Molecular Immunology. 33・11/12. 925-932 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miki Nakao: "Terminal components of carp complement constituting a membrane attack complex" Molecular Immunology. 33・11/12. 933-937 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Rei Obo: "C3-receptors on the head kidney leukocytes of carp(Cyprinus carpio)" Proceeding of the Complement Symposium. 33. 145-147 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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