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Development of bovine eggs collected from ovaries of live animals with ultrasound-guided system

Research Project

Project/Area Number 08556045
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Applied animal science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YAMADA Masayasu (1997-1998)  Kyoto univ.Grad.School of Agri., Associate Professor, 農学研究科, 助教授 (10243073)

内海 恭三 (1996)  京都大学, 農学部, 教授 (90033266)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Naohiko  Gifu Prefectural Beef cattle Res.Inst., Researcher, 研究員
FUJITANI Yasuhiro  Osaka Prefectural Agri.and Foret.Res.Center, Researcher, 研究員
MINAMI Naojiro  Kyoto Univ.Grad.School of Agri., Researcher, 農学研究科, 助手 (30212236)
HOSOI Yukihiko  Kinki univ.Res.Inst.Bio.-Oriented Sci.and Tech., Assoc.Prof., 生物理工学部, 助教授 (70192739)
SUZUKI Tatsuyuki  Yamaguchi Univ.School of Agriculture, Professor, 農学部, 教授 (00216409)
後藤 和文  鹿児島大学, 農学部, 教授 (30162142)
山田 雅保  京都大学, 農学部, 助教授 (10243073)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsBovine oocyte / transvaginal collection / ultrasound guided-system / superovulation / follicular aspiration / anti-inhibin antibody / in vitro maturation / midkine / 超音波画像 / 卵胞液吸引 / インヒビン / 細胞質成熟促進活性 / バキュロウイルス / 細胞質成熟促進因子 / 遺伝子発現 / 体外培養 / RT-PCR法 / CCDイメージカメラ
Research Abstract

To develop the system for efficient production of live stock animals, especially cattle with superior genetic traits, attempts have been made to produce the embryos by in vitro culture system, including in vitro maturation and in vitro fertilization of oocytes collected from ovaries from live animals with transvaginal aspiration using ultrasound echo system with a linear probe. We first determined a suitable condition, especially aspiration pressure and needle diameter for bovine follicular oocyte collection with transvaginal aspiration. As a result, we found that when the aspiration condition of 18- gauge needle at 80 mmHg was used, the highest oocyte recovery rates were obtained. In the second experiments, to stimulate the development of small sized follicles in cow ovaries, we tried to remove dominant follicles in low responder cows to superovulatory stimulation with follicular aspiration using ultrasound-guided aspiration techniques. It was found that the aspiration of follicular f … More luids of dominant follicles greatly stimulates the development of small-sized follicles, and moreover that most of the small follicles developed result in the ovulation with superovulatory treatments. Thus, with follicular aspiration, recovery rates of embryos after superovulatory treatments followed by artificial insemination were highly improved in low responder cows. Furthermore, we found that injection of anti-inhibin antibody raised against procin inhibin peptide to such cows resulted in similar response to superovulatory treatments to the case of follicular aspiration. In the third experiments, we found that in vitro maturation of oocytes collected from small sized follicles of cow ovaries in medium containing fractions of bovine follicular fluids separated by heparin affinity chromatography promotes subsequent development to the blastocyst stage after in vitro fertilization. Furthermore, it was found that the promoting activities were exerted with midkine, a novel heparin binding growth /differentiation factor in bovine follicular fluids. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] K.S.Kim: "The effects of follicalar fluid on invitro maturation, oocyte fertilization and development of bovine embryos" Theriogenology. 45. 787-799 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Fujitani: "Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos" J.Anim.Sci.75. 483-489 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Nishikimi: "Evaluation of acrosornal status of bovine spermatozoa using concanavalin A lectin." Theriogenology. 48. 1007-1016 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.S.Kim: "Follicle cells affect the fertilizability and developmental competency of bovine oocytes in vitro" Reprod.Fert.Dev.9. 763-766 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto: "Effects of cumulus cell density during in vitro mataration on the developmental competence of bovine oocytes." Theriogenology. 49. 1451-1463 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Iwata: "Effects of antioxidants on the development of bovine LL14/IVF embryos in various concentration of glucose." Theriogenology. 50. 365-375 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto: "The collection of bovine cumulus-oocyte conplexes from ovarite of staughtered of live cows." Theriogenology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto: "Ultrasound-guided follicle aspiration : Effect of the freguency of a linear transvaginal probe on the collection of bovine oocytes." Theriogenology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Nishikimi: "Reproductive Biology Update" Shoukadoh Boodsellers Company, 5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Minami: "Reproductive Biology Update" Shoukadoh Booksellers Company, 9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Yamada: "Reproductive Biology Update" Shoukadoh Boodsellers Company, 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Parnpai: "Reproductive Biology Update" Shoukadoh Boodsellers Company, 6 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Nishikimi, J.Mukai, N.Minami and M.Yamada: "Expression and activation of nuclear factor kappa B during mouse preimplantation development." Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-. Shoukadoh Booksellers Company. 183-187 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Minami, A.Ohashi, K.Sasaki, T.Wada and M.Yamada: "Second cell cycle and cell adhesion of mouse embryos developed in an oviductal environment in vitro." Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-. Shoukadoh Booksellers Company. 345-353 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Yamada, A.Nishikimi, N.Uekawa, K.Hoshino and N.Minami: "Extraembryonic environment and development of preimplantation rat embryos." Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-. Shoukadoh Booksellers Company. 355-364 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] R.Parnpai, N.Minami and M.Yamada: "Current status of research for the establishment of bovine embryonic stem (ES) cells." Reproductive Biology Update-Novel Tools for Assessment of Environmental Toxicity-. Shoukadoh Booksellers Company. 383-388 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.S.Kim, N.Mitsumizo, K.Fujita and K.Utsumi: "The effects of follicular fluid on in vitro maturation, oocyte fertillization and development of bovine embryos." Theriogenology. 45. 787-799 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Fujitani, K.Kasai, S.Ohtani, K.Nishimura, M.Yamada and K.Utsumi: "Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos." J.Anim.Sci.75. 483-489 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Nishikimi, M.Yamada, N.Minami and K.Utsumi: "Evaluation of acrosomal status of bovine spermatozoa using concanavalin A lectin." Theriogenology. 48. 1007-1016 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.S.Kim, N.Minami, M.Yamada and K.Utsumi: "Follicle cells affect the fertilizability and developmental competency of bovine oocytes in vitro." Reoprod.Fert.Dev.9. 763-766 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto, K.Saeki, Y.Nagao, N.Minami, M.Yamada and K.Utsumi: "Effects of cumulus cell density during in vitro maturation on the developmental competence of bovine oocytes." Theriogenology. 49. 1451-1463 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Iwata, S.Akamatsu, N.Minami and M.Yamada: "Effects of antioxidants on the development of bovine IVM/IVF embryos in various concentration of glucose." Theriogenology. 50. 365-375 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto, R.Takakura, M.Kishi, T.Sudo, N.Minami and M.Yamada: "Ultrasound-guided follicle aspiration : The collection of bovine cumulus-oocyte complexes from ovaries of slaughtered or live cows." Theriogenology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Hashimoto, R.Takakura, N.Minami and M.Yamada: "Ultrasound-guided follicle aspiration : Effect of the frequency of a linear transvaginal probe on the collection of bovine oocytes." Theriogenology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hashimoto,S.: "Ultrasound-Guided Follicle Aspiration : The Conection of Bovine Cumulus-oocyte Complexes From Ovaries of Slaughtered or Live Cows." Theriogenology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hashimoto,S.: "Ultrasound-Guided Follicle Aspiration : Effect of the Frequency of a Linear Transvaginal Probe on the Cellection of Bovine Oocytes" Theriogenology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwata,H.: "Allopurinol, an inhibitor of xanthine oxidase, improves the development of IVM/IVF bovine embryos in vitro." Theriogenology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hashimoto,S.: "Effects of Cumulus Cell Density During In vitro Mataration on the Developmental Competence of Bovine Oocytes" Theriogenology. 49. 1451-1463 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Iwata,H.: "Effects of Antioxidants on the Development of Bovine IVM/IVF Embryos in Variaus Concentrations of Glucose." Theriogenology. 50. 365-375 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kobayashi,S.: "Embryo Transfer of Bovine Embryos Cellected from Cows with Super-Ovulatory Treatments following aspiration of a dominant follicle." Jpn.J.Embryo Transfer. 20. 78-83 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishikimi,A.: "Evaluation of acrosomal status of bovine spermatozoa using concaravalin A" Theriogenology. 48・7. 1007-1016 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 錦見昭彦: "H-Y抗原をコードする遺伝子Smcyの産物を認識する抗ペプチド抗体作製の試み" 日本胚移植学雑誌. 19・2. 111-116 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 青木義和: "ウシの繁殖生理状況の違いが超音波経膣採取成績に及ぼす影響" 日本胚移植学雑誌. 19・2. 117-124 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 錦見昭彦: "総説:H-Y抗原" 日本胚移植学雑誌. 19・3. 185-193 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamada,M.: "Reproductive Biology Update" Nakanishi Printing Co.,Ltd.Kyoto,Japan, 10 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小林修一: "ウシ発情周期における卵胞動態の超音波画像診断装置による解析と過剰排卵反応" 日本畜産学会報. 68・1. 45-53 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Utsumi: "Effect of passive immunization on growth of ollides and subsequent superovulatory response in Japanese Black Cattle." Theriogenology. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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