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Development of a near field scanning optic microscope using evanescent light for observation of molecular movement in an aqueous solution.

Research Project

Project/Area Number 08557002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKitasato University

Principal Investigator

FUTAESAKU Yutaka  Kitasato Univ., Sch.Allied Health Sci., Dept.Histol., Prof., 医療衛生学部, 教授 (50014197)

Co-Investigator(Kenkyū-buntansha) OSAWA Hisao  Nikon Corp., Res.Devel.Dept., Chief Manager, 技術開発部, 係長
SUZUKI Masatoshi  Nikon Corp., Instr.Dev., SPM Sec., Sen.Assist.Manager, 顕微鏡開発部, 係長
SEKIYA Kachiko  Kitasato Univ., Sch.Pharmac., Lab.Electron Microsc., Assoc.Prof., 薬学部, 講師 (30050579)
YANO Misai  Kitasato Univ., Sch.Allied Health Sci., Dept.Histol., Assist.Prof., 医療衛生学部, 助手 (40255360)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥18,600,000 (Direct Cost: ¥18,600,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥15,300,000 (Direct Cost: ¥15,300,000)
KeywordsNSOM / evanescent light / in solution / AFM / optical scattering / SLO / biological membrane / observation of molecular movement / エバネッセント光顕微鏡 / AFM / SLO / 蛋白分子 / ウェットチャンバー / 蛋白質分子 / 動態
Research Abstract

1) Evaluation of the near field scanning optic microscope using evanescent light
(1) An optical microscope ; A probe of AFM was set on a specimen under an inverted light microscope for seeking appropriate region to observe.
(2) An atomic force microscope (AFM) ; A probe can scan 100 mutm maximum and the resolution is less than 10 nm.
(3) A near field scanning optic microscope using evanescent light (NSOM) ; A specimen was mounted on a lucent stage. An evanescent light tunneled through a surface of the specimen, when the laser light irradiated the rear side of the stage within the critical angle. The evanescent light was scattered by the AFM probe. The scattered light was collected by an objective lens and detected by a photomultiplier. The signal was amplified by a two phase lock-in amplifier and displayed on CRT.
2) Observation of Molecular Movement in an Aqueous Solution
We can detect a scatted light from an evanescent light. A hundred cycles per a minute is very slow to form an image to catch the movement of molecules.
3) Problem remains to be clarified
(I) We have large background-noise from a specimen which reflect the laser beam.
(2) Formation of a SLO-ring is completed within a few minutes. For delaying the reaction time, we set temperature of a specimen low using a cooling system. The cooler created another problem of thermal drift.
(3) We drive to scan an AEM probe instead of a specimen chamber since quick scanning was required.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] K.Sekiya: "Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin, a Thiol-Activated Hemolysin from Clostrudium botulnum" Infect. and Immun.66(6). 2987-2990 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sekiya: "Characterization of th Damage to Membranes Caused by Bacterial Cytolysins" J. Electron Microsc.47(1). 543-552 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku: "An Evanescent Light Probe Microscope (SNOM) for Biological Application" Proc. 14th Intl. Cong. Electr. Microsc.1. 505-506 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku: "Development of Non-Carbon Support Films for Electron Spectroscopic Imaging (ESI) in Biology" Cell. and Mol. Biol.45(予定). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku: "Biological Meaning of Electron Spectroscopic Imaging (ESI) and Its Application" Proc. 9th Japan-China Electr. Microsc. Sym.(予定). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sekiya, H.Danbara, Y.Futaesaku, A.Haque, N.Sugimoto, and M.Matsuda: "Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin, a Thiol-Activated Hemolysin from Clostrudium botulnum" Infect.and Immun.66 (6). 2987-2990 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sekiya and Y.Futaesaku: "Characterization of the Damage to Membranes Caused by Bacterial Cytolysins" J.Electron Microsc.47 (1). 543-552 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku, M.Yano, K.Sekiya, M.Suzuki, and H.Ohsawa: "An Evanescent Light Probe Microscope (SNOM) for Biological Application" Proc.14th Intl.Cong.Electr.Microsc.1. 505-506 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku, M.Ueno, Y.Kozuka, K.Tone, T.Hashikawa, T.Akagai, H.Akahori, K.Adachi, N.Baba, T.Oikawa, T.Hanada, K.Yase, and K.Sekiya: "Development of Non-Carbon Support Films for Electron Spectroscopic Imaging (ESI) in Biology" Cell.and Mol.Biol.45. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Futaesaku Y.Futaesaku, K.Tone, S.Okuda, T.Hashikawa, T.Akagi, T.Hanada, K.Yase, and K.Sekiya: "Biological Meaning of Electron Spectroscopic Imaging (ESI) and Its Application" Proc.9th Japan-China Electr.Microsc.Sym.(1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Sekiya: "Formation of Ring-Shaped Structures on Erythrocyte Membranes after Treatment with Botulinolysin,a Thiol-Activated Hemolysin from Clostrudium botulnum" Infect.and Immun.66(6). 2987-2990 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Sekiya: "Characterization of th Damage to Membranes Caused by Bacterial Cytolysins" J.Electron Microsc.47(1). 543-552 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Futaesaku: "An Evanescent Light Probe Microscope (SNOM) for Biological Application" Proc.14th Intl.Cong.Electr.Microsc.1. 505-506 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Futaesaku: "Development of Non-Carbon Support Films for Electron Spectroscopic Imaging (ESI) in Biology" Cell.and Mol.Biol.45(予定). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Futaesaku: "Biological Meaning of Electron Spectroscopic Imaging (ESI) and Its Application" Proc.9th Japan-China Electr.Microsc.Sym.(予定). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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