| Project/Area Number |
08557007
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Kobe University |
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Principal Investigator |
OKAMURA Hitoshi Kobe University, Department of Anatomy and Brain Science, Professor, 医学部, 教授 (60158813)
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| Co-Investigator(Kenkyū-buntansha) |
T.INOUYE Shin-Ichi Yamaguchi University, Faculty of Science, Professor, 理学部, 教授 (10274151)
SHINOHARA Hiroaki Okayama University, Department of Bioscience and Biotechnology, Faculty of Engin, 工学部, 助教授 (60178887)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1998: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | clock genes / luciferase- / mPer1-promotor / bioaffinity binding / ルシフェラーゼ / 時計遺伝子 / サーカディアンリズム / フォトン検出装置 / Rat1 / 視交叉上核 / ピリオド遺伝子 / 概日リズム |
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| Research Abstract |
It is considered that protein assembly on solid substrates is the important key technology to fabricate high performance biosensing systems and bioelectronics devices. The purpose of this study is to develop novel protein assembling method using bioaffinity binding and dissociation. The author paid attention to bioaffinity binding reaction between biotin analogues and avidin molecule. It is expected that biotin analogue-modified protein molecules are assembled on the solid supports by using avidin molecules as binders and that the constructed protein complexes are decomposed by the exchange reaction with free biotin molecules. This assembling technique might be available to fabricate recycle usable biosensing system and intelligent bioreactor system in which biocatalysis is controlled by the addition of ligand molecules as stimulation signal.
Bioaffinity binding and dissociation reactions of avidin and biotin- or biotin analogue-modified proteins were characterized with evanescent wave-
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exciting fluorescence measurement. Dissociation of avidin and biotin analogue-modified protein complexes was certainly induced by the free biotin addition. It was demonstrated that biotin sensing is possible in the concentration range from 1x10^<-9> M to 1x10^<-5> M by the measurement of dissociation degree. The data further suggested that the biotin concentration for dissociation induction is dependent on the bioaffinity between the biotin analogue and avidin.
At second, It was demonstrated that redox coenzyme-modified avidin molecules were assembled on the biotin-modified electrode surface and functioned as bioelecfrocatalyst for NADH oxidation. Dissociation of themodified avidin from the biotin-modified electrode surface was also induced by biotin addition.
During three years we cloned putative mammalian clock genes (mPer1, mPer2, mPer3 and mTim). mPer1-promotor-Luciferasefusion gene was transfected to the Rat1 fibroblast cells. We observed the change of luminescence at single cell level by a sensitive photon detector. After serum shock, the luciferin luminescence begins to show the rhythm in a period length between 20 to 28 hours. Less
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