| Project/Area Number |
08557019
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Experimental pathology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YOSHIKI Takashi Hokkaido Univ.Sch.of Med., Pro., 医学部, 教授 (60220612)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Hitoshi Hokkaido Univ.Sch.of Med., Inst., 医学部, 助手 (20232192)
WAKISAKA Akemi Hokkaido Univ.Sch.of Med., Ass.Pro., 医学部, 助教授 (90113646)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1997: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1996: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | HTLV-I / Disease model / Transgenic rat / Apoptosis / HTLV-I / モデル動物 / TNFα / トランスジュニック / ラット |
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| Research Abstract |
Human T lymphocyte virus type I (HTLV-I) is associated with a number of diverse clinical disorders. To investigate the pathogenesis of the HTLV-I related disease, rats carrying HTLV-I (HAM rats) and those transgened with env-pX gene (env-pX rats) were investigated.
1) HAM rats develop a chronic progressive myelopathy after long incubation periods. The myelopathy is caused by demyelination based on apoptosis of oligodendrocytes. Microglias were infected with HTLV-I.High expression of pX mRNA and TNF-alpha is observed before apoptosis in the diseased spinal cord, while low of bcl-2.
2) env-pX rats develop a wide spectrum of collagen vascular and autoimmune diseases. Peripheral T lymphocytes of these rats express high amount of CD80/86 and ICAM-I showing high response to various antigens. The T lymphocytes infiltrated to the diseased organ/tissu were oligoclonal. Bone marrow transplantation experiments suggest that at least 3 types of pathogenic process are concerned to develop diseases.
3) Ick promotor env-pX rats show lymphoid tissue specific expression of pX mRNA.They develop thymomas but not ATL until now.
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