• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a new technology using synthetic malarial peptides for immunological analysis of an endemic population

Research Project

Project/Area Number 08557021
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 寄生虫学(含医用動物学)
Research InstitutionGunma University

Principal Investigator

SUZUKI Mamoru  Gunma University School of Medicine, Professor, 医学部, 教授 (60056033)

Co-Investigator(Kenkyū-buntansha) KATAKAI Ryoichi  Faculty of Engineering, Gunma University, Professor, 工学部, 教授 (10008500)
SATO Kumiko  Gunma University School of Health Sciences, Professor, 医学部, 教授 (80008268)
KANO Sigeyuki  Research Institute, International Medical Center of Japan, Director, 適性技術開発・移転研究部, 部長 (60233912)
YOSHIDA Masaru  Japan Atomic Energy Research Institute, Chief Investigator, 高崎研究所, 主任研究員
OKU Hiroyuki  Faculty of Engineering, Gunma University, Assistant Professor, 工学部, 助手 (20301749)
米澤 信行  群馬大学, 工学部, 助教授 (10167033)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥15,500,000 (Direct Cost: ¥15,500,000)
Fiscal Year 1998: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1997: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥8,500,000 (Direct Cost: ¥8,500,000)
Keywordsmalaria / synthetic antigen / immunology / diagnosis / sporozoite / enolase / 診断
Research Abstract

In this research, we synthesized the following two peptides first, Boc-GIGNPNAGIG-Oet and cyclo[(NPNAGAG)ィイD22ィエD2] with regard to the basic structure of the circum sporozoite protein, (NANP)ィイD2nィエD2 Structure of the peptides were analyzed by Nuclear Magnetic Resonance, Fourier-transform Infraredspectrum, and Circular Dichroism Spectroscopy, and it was revealed that those peptides had β-structure within the polypeptides which would work as an appropriate antigenic structure for the detection of the malarial antibody. When we examined their antigenicity by fluorescence-ELISA by applying malaria patients' sera from Thailand, quite a nice reaction was observed suggesting their potential importance as diagnostic materials.
Another synthetic polypeptides studied in this research was a part of enolase, a glycolytic enzyme. We analyzed the tertially structure of enolase with reference to the crystal structure of yeast enolase. The identity of the peptides were calculated as 61% and we concluded that we could built up the structure of Plasmodial enolase by computer graphics. Then we chose a part of the polypeptides which consisted of Asn with β-turn structure: GFAPNILNANEALDLL. The synthetic polypeptides of this helical structure was also proved to be a good antigenic molecule for the detection of malarial antibody. It is also proved that this molecule was highly reactive against the sera from complicated malaria patients, but less reactive against those from mild malaria patients. We, thus, regarded this molecule as a good material for us to develop appropriate diagnostic technology not only for individuals, but also for the evaluation of epidemiological situation of endemic areas.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] 狩野繁之: "マラリアの制圧をめざす"Kitakanto Medical Journal. 47・5. 365-367 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katakai,R.: "Hydrophobic site-specific control of the volume phase transition of hydrogels"Macromolecules. 31・10. 3383-3384 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano, S.: "Serological evaluation of malaria patients in Thailand, with paticular refeence to the reactivity against a 47kD antigenic polypeptide of Plasmodium falciparum"Tokai J Exp Clin Med. 23・2. 97-98 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto, Y.: "Different plasma levels of circulating cell adhesion molecules in falciparum malaria patients according to deisease severity"Tokai J Exp Clin Med. 23・2. 79-80 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鈴木守: "世界におけるマラリア"化学療法の領域. 14・5. 783-787 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鈴木守: "マラリアの感染に伴う腎障害"腎と透析. 44・3. 384-389 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Norazmi, M.N., Kano, S., Alias, A., Abdullah, M.S., Suzuki, M.: "Reactivity of sera from patients with acute Plasmodium falciparum and P. vivax infections with an antigen preparation from a P. falciparum isolate : -Mutually exclusive reactivity with a 47kD and a 29kD band respectively"Jpn. J. Trop. Med. Hyg.. 24. 237-239 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawai, S., Kano, S., Chang, C., Suzuki, M.: "The effects of pyronaridine on the morphology of Plasmodium falciparum in Aotus Trivirgatus"Am. J. Trop. Med. Hyg.. 55. 223-229 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, M., Asano, M., Safranj, A., Omichi, H., Spohr, R.. Vetter, J., Katakai, R.: "Novel thin film with cylindrical nanopores that open and close depending on temperature : first successful synthesis"Macromolecules. 29. 8987-8989 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katakai, R., Yoshida, S., Hasegawa, S., Iijima, Y., Yonezawa, N.: "Phase transition of hydrogels by "pinpoint-variation" of polymer side chains"Macromolecules. 29. 1065-1066 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rivera, P.T., Kano, S., Miguel, E.C,, Suzuki, M.: "A 23kD Molecule of Plasmodium falciparum binds specific IgG from splenomegalic, parasitemic, but asymptomatic children - A pilot Study in a malarious community in Palawan the Philippines -"Jph. J. Trop. Med. Hyg.. 25. 1-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano, S., Tsuzuki, K., Inaba, H., Onda, T., Masuda, G., Suzuki, M.: "DNA diagnosis of Plasmodium falciparum malaria by Single-Tube PCR"J. Jpn. Assoc. Inf. Dis.. 71. 1090-1092 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamaguchi, S., Kubota, T., Yamagishi, T., Okamoto, K., Izumi, T., Takada, M., Kano, S., Suzuki, M., Tsuchiya, J., Naruse, T.: "Severe thrombocytopenia suggesting immunological mechanisms in two cases of vivax malaria"Am. J. Hematol.. 56. 183-186 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, M., Asano, M., Suwa, T., Reber, N., Spohr, R., Katakai, R.: "Creation of thermo-responsive ion-track membranes"Advanced Materials. 9. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagaoka, N., Yoshida, M., Asano, M., Suwa, T., Kubota, H., Katakai, R.: "Simultaneously occurring process of radiation-induced polymerization, crosslinking, and degradation of n-isopropylacrylamide"Journal of Polymer Science : Part A: Polymer Chemistry. 35. 3075-3077 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, M., Asano, M., Omichi, H., Spohr, R., Katakai, R.: "Substrate-specific functional membranes based on etched ion tracks"Radiation Measurements. 28. 799-810 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Negishi, M., Yoshida, M., Miyamoto, Y., Miyajima, M., Asano, M., Katakai, R.: "ph-sensitive release of ketoprofen from sodium laurate-loaded poly (acryloyl-l-proline methyl ester) gels"Pharmaceutical Sciences. 3. 129-132 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, K., Nakazawa, T., Sato, Y., Nakano. M., Nojiri, M., Ohno, J.: "Mite-specific IgE antibody carriage among an adult population in Gunma Prefecture"Occup. Environ. Allergy. 4. 37-44 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, K., Nakazawa, T., Sahashi, N., Kochibe, N.: "Yearly and seasonal changes of IgE to Japanese cedar pollen in young generation"Ann. All. Asthma Immunol.. 79. 57-61 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano, S., Onda, T., Matsumoto, Y., Buchachart, K., Krudsood, S., Looareesuwan, S., Aikawa, M., Suzuki, M.: "Serological evaluation of malaria patients in Thailand : Antibody response against electrophoresed antigenic polypeptides of Plasmodium falciparum"Southeast Asian J. Trop. Med. Pub. Hlth.. 29. 341-343 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sato, K., Nakazawa, T., Miyazaki, Y.: "Japanese cedar pollen-specific IgE antibody carriage among an adult population in Gunma Prefecture"Occup. Environ. Allergy. 5. 21-19 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katakai, R., Saito, K., Sorimachi, M., Hiroki, A., Kinuno, T., Nakajima, T., Shimizu, M., Kubota, H., Yoshida, M.: "Hydrophobic site-specific control of the volume phase transition of hydrogels"Macromolecules. 31. 3383-3384 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano, S., Onda, T., Matsumoto, Y,. Looareesuwan, S., Aikawa, M., Suzuki, M.: "Serological evaluation of malaria patients in Thailand, with particular reference to the reactivity against a 47kD antigenic polypeptide of Plasmodium falciparum"Tokai J Exp Clin Med. 23. 97-98 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto, Y., Naya, T., Darko, C.A., Katakai, Y., Kano, S., Suzuki, M., Wilairatana, P., Looareesuwan, S., Brittenham, G.M., Aikawa, M.: "Different Plasma Levels of circulating cell adhesion molecules in falciparum malaria patients according to disease severity"Tokai J Exp Clin Med. 23. 79-80 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano,S.: "Serological evaluation of malaria patients in Thailand:antibody response against electrophoresed antigenic polypeptides of Plasmodium falciparum." Southeast Asian J Trop Med Pub Hlth. 29. 341-343 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kano,S.: "Serological evaluation of malaria patients in Thailand,with particular reference to the reactivity against a 47 kD antigenic polypeptide of Plasmodium falciparum." Tokai J Exp Clin Med. 23. 97-98 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto,Y.: "Different plasma levels of circulating cell adhesion molecules in falciparum malaria patients according to disease severity." Tokai J Exp Clin Med. 23. 79-80 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo,T.: "Characteristics of acrylic acid and N-isopropylacrylamide binary monomers-grafted polyethylene film synthesized by photografting." J Appl Polym Sci. 67. 2057-2064 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kondo,T.: "Effect of ethylene glycol dimethacrylate derivatives on photografting of methacrylic acid on low-density polyethylene." Eur Polym J. 34. 1099-1103 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katakai,R.: "Hydrophobic site-specific control of volume phase transition of hydrogels." Macromolecules. 31. 3383-3384 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shigeyuki Kano: "DNA diagnosis of Plasmodium falciparum malaria by Single-Tube PCR" Japanese Journal of Infectious Diseases. 71・10. 1090-1092 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigeyuki Kano: "Serological evaluation of malaria patients in Thailand:Antibody response against electrophoresed antigenic polypeptides of Plasmodium falciparum" Southeast Asian J Trop Med Pub Hlth. (in print). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Pilarita Tongol-Rivera: "A 23kD Molecule of Plasmodium falciparum binds specific IgG from splenomegalic,parasitemic,but asymptomatic children" Japanese Journal of Tropical Medicine and Hygiene. 25・1. 1-5 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Yoshida: "Dependency of volume phase transition temperature of poly(methacryloyl-L-proline methyl ester)gel on hydrophobic tail length of anionic surfactants" Macromolecules. 30. 2795-2796 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Yoshida: "Creation of thermo-responsive ion-track membranes" Advanced Materials. 9. 757-758 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Noriyasu Nagaoka: "Crosslinking,degradation and re-crosslinking of N-isopropylacrylamide in the course of radiation-induced polymerization" Journal of Polymer Science Part A,Polymer Chemistry. 35. 3075-3077 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mohd-Nor Norazmi: "Reactivity of sera from patients with acute Plasmodium falciparum and P. vivax infections with an antigen preparation from a P. falciparum isolate" Japanese Joumal of Tropical Medicine and Hygiene. 24(4). 237-239 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Pilarita Tongol-Rivera: "A 23kD Molecule of Plasmodium falciparum binds specific IgG from splenomegalic. parasitemic,but asvmptomatic children" Japanese Joumal of Tropical Medicine and Hygiene. 25(1)(in print). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 狩野繁之: "マラリアワクチン" 臨床と微生物. 24(2)(in print). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Satoru Kawai: "The effects of pyronaridine on the morphology of Plasmodium falciparum in Aotus Trivirgatus" American Joumal of Tropical Medicine and Hygiene. 55(2). 223-229 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tomohide Kitaguchi: "Analysis of roles of natural killer cells in defense against Plasmodium chabaudi in mice" Parasitology Research. 82. 352-357 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木守: "マラリア-emergingまたはre-emerging infectious diseaseとしてのマラリア-" 最新医学. 52(1). 88-92 (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi