| Project/Area Number |
08557043
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Neurology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TASHIRO Kunio School of Med., Hokkaido University, Prof., 医学部, 教授 (90002154)
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| Co-Investigator(Kenkyū-buntansha) |
HIBI Nozomi SRL,Chief Researcher, 主席研究員
TSUKADA Hiroshi SRL,Head Researcher, 研究部長
MORIWAKA Fumio School of Med., Hokkaido University, Asso.Pro., 医学部, 助教授 (30142722)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1996: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | NEUROMUSCULAR DISEASES / MUSCLE ENZYME / CARBONIC ANHYDRASE-III / MONOCLONALANTIBODY / MYOCARDIAL INFARCTION / MYOGLOBIN / Myoglobin / CA-III / 心筋障害 / Carbonid anhydrase / 炭酸脱水水素アイソザイム1型 / モノクローナク抗体 / ELISA / 測定方法 / 筋疾患マーカー / Garbonic anhydrase III |
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| Research Abstract |
We studied the diagnostic strategies of carbonic anhydrase-III(CA-III) in the neuromuscular diseases and the possibility of the clinical application of CA-III in other medical areas. Furthermore, the purification of monoclonal antibody and the establishment of measurement system using it ware also carried out.
The diagnostic strategies of CA-III in the neuromuscular diseases were analyzed by the measurement of serum levels of CA-III in 353 patients with neuromuscular diseases, 99 healthy controls and 110 disease controls. The values of sesrum CA-III in healthy and disease controls were below 20 ng/ml and patients with progressive muscular dystrophy and polymyositis showed highly elevated CA-III levels and cases of amyotrophic lateral sclerosis and distal myopathy disclosed moderately elevations of CA-III.
The purification of monoclonal antibody was carried out in BALB/C mouse using by cultured P3U1 oil line Most of the purified monodonal antibody were IgGl and the antibody titer were 2.9x10^2 to 3.2x10^4. The meausrement system using the pun lied monodonal antibody disclosed better sensitivity than that of polycolonal antibody.
The clinical application of CA-III in myocardial infarction using the ratio of CA-III and myoglobin were also evaluated and it seemed to be useful
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