Purification of a novel migration factor for vascular smooth muscle cells, derived from omentum and its application

• Project/Area Number: 08557059
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 展開研究
• Research Field: 内分泌・代謝学
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: SAITO Yoshihiko Kyoto Univ., Grad.Sc.of Med., Assistant Professor, 医学研究科, 助手 (30250260)
• Co-Investigator(Kenkyū-buntansha): NIYAMOTO Tsumoru Biol.-Pharmacological.Res.Lab., JAPAN TOBACCO,INC., Central Pharmaceutical.Res.I, 医薬総合研究所・生物研究所, 所長 ; NISHIMURA Kazunobu Kyoto Univ., Grad.Sc.of Med., Associate Professor, 医学研究科, 講師 (70252450) ; NAKAO Kazuwa Kyoto Univ., Grad.Sc.of Med., Professor, 医学研究科, 教授 (00172263)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥13,000,000 (Direct Cost: ¥13,000,000); Fiscal Year 1997: ¥6,400,000 (Direct Cost: ¥6,400,000); Fiscal Year 1996: ¥6,600,000 (Direct Cost: ¥6,600,000)
• Keywords: omentum / migration factor of USMC / phosphadilglycerol / 大網 / 血管内皮細胞 / 血管新生 / 内皮細胞遊走活性

Research Abstract

Growing evidence indicates that adipose tissues have potent angiogenic activity. In the present study, we tried to purify the new angionenic factor from omentum monitoring the migration activity of vascular smooth muscle cells. Three kg of omentum was homogenized in the 15 little of methanol. After four steps of purification procedure including liquid- ; liquid partition chromatography, preparative thin-layr chromatography and two kinds of high-performance liquid chromatography, 0.3mg of purified product was obtained. Spectral analysis using fast atom bombardment mass spectrometry (FAB-MAS) ans nuclear magnetic resonance spectrometry revealed its molecular weight is 747.9 and it is 1-Palmitoyl-2-oleoyl-phosphatidylglycerol. Migration activity of synthesized 1-Palmitoyl-2-oleoyl-phosphatidylglycerol was detected. We also examined the migration activity of other phospholipids. Phosphatidylethanolamine and phosphatidylserine have migration activity, though phsophatidylchorine does not.
In the present study, 1-Palmitoyl-2-was purified as a migration factor derived from omentum.

Research Products

• [Publications] M.Harada et. al.: "Signilicance of Ventricular Myocytes and Nonmyocytes Interaction During Cardiocyte Hytertrophy.-Evidence for Endothelin-1 as a Paracrine Hypertrophic Factor from Cardiac Nonmyocytes-" Circulation. 96. 3737-3744 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] T.Wallen et. al.: "Brain natriuretic peptide predicts mortality in the elderly." Heart. 77. 264-267 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Yoshibayashi et.al.: "Elevated levels of adrenomedullin in primary and secondary pulmonary hypertension in patients <20 years of age." Am J Cardiol. 79. 1556-1556 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Miyamoto et.al.: "Endothelial Nitric Ozide Synthase Gene is Positively Associated With Essential Hypertension." Hypertension. (in press). (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M.Suda et.al.: "Skeletal overgrowth in transgenic mice that overexpress brain natriuretic peptide." Proc.Natl.Acad.Sci.USA. (in press). (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Y.Shimasaki et.al.: "Association of the Missense Glu 298 Asp Mutation of the Endothelial Nitric Oxide Synthase Gene With Myocardial Infarction" J.Am.Coll.Cardiol.(in press). (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Harada M,Itoh H,Nakagawa O,Ogawa Y,Miyamoto Y,Kuwahara K,Ogawa E,Igaki T,Yamashita J,Masuda I,Yoshimasa T,Tanaka I,Saito Y,Nakao K.: "Significance of Ventricular Myocytes and Nonmyocytes Interaction During Cardiocyte Hytertrophy. -Evidence for Endothelin-1 as a Paracrine Hypertrophic Factor from Cardiac Nonmyocytes-" Circulation. 96. 3737-3744 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Wallen T,Landahl S,Hedner T,Nakao K,Saito Y.: "Brain natriuretic peptide predicts mortality i the elderly." Heart. 77. 264-267 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshibayashi M,Kamiya T,Kitamura K,Saito Y,Kangawa K,Nishikimi T,Matsuoka H,Eto, Matsuo H.: "Elevated levels of adrenomedullin in primary and secondary pulmonary hypertension in patients <20 years of age." Am J Cardiol.79. 1556-1558 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyamoto Y,Saito Y,Kajiyama N,Yoshimura M,Shimasaki Y,Nakayama M,Kamitani S,Harada M,Ishikawa M,Kuwahara K,Ogawa E,Hamanaka I,Takahashi N,Kaneshige T,Teraoka H,Akamizu T,Azuma N,Yoshimasa Y,Yoshimasa T,Itoh H,Masuda I,Yasue H,Nakao K.: "Endothelial Nitric Ozide Synthase Gene is Positively Associated With Essential Hypertension." Hypertension. (in press). (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Suda M,Ogawa Y,Tanaka K,Tamura N,Yasoda A,Takigawa T,Uehira M,Nishimoto H,Itoh H,Saito Y,Shiota K,Nakao K.: "Skeletal overgrowth in transgenic mice taht overexpress brain natriuretic peptide." Proc.Natl.Acad.Sci.USA. (in press). (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Shimasaki Y,Hirofumi Y,Michihiro Y,Nakayama M,Kugiyama K,Ogawa H,Harada E,Masuda T,Koyama W,Saito Y,Miyamoto Y,Ogawa Y,Nakao K.: "Association of the Missense Glu 298 Asp Mutation of the Endothelial Nitric Oxide Synthase Gene With Myocardial Infarction." J.Am.Coll.Cardiol.(in press). (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M.Harada et.al.: "Signilicance of Ventricular Myocytes and Nonmyocytes Interaction During Cardiocyte Hytertrophy. Evidence for Endothelin-1 as a Paracrine Hypertrophic Factor from Cardiac Nonmyocytes-" Circulation. 96. 3737-3744 (1997)
• [Publications] T.Wallen et.al.: "Brain natriuretic peptide predicts mortality in the elderly." Heart. 77. 264-267 (1997)
• [Publications] M.Yoshibayashi et.al.: "Elevated levels of adrenomedullin in peimary and secondary pulmonary hypertension in patients<20 years of age." Am J Cardiol. 79. 1556-1558 (1997)
• [Publications] Y.Miyamoto et.al.: "Endothelial Nitric Ozide Synthase Gene is Positively Associated With Essential Hypertension." Hypertension. (in press). (1998)
• [Publications] M.Suda et.al.: "Skeletal overgrowth in transgenic mice that overexpress brain natriuretic peptide." Proc.Natl.Acad.Sci.USA. (in press). (1998)
• [Publications] Y.Shimasaki et.al.: "Association of the Missense Glu 298 Asp Mutation of the Endothelial Nitric Oxide Synthase Gene With Myocardial Infarction" J.Am.Coll.Cardiol.(in press). (1998)
• [Publications] T.Nishikimi et.al.: "Relationship between left ventricular geometry and natriuretic peptide levels in essential hypertension." Hypertension. 28(1). 22-30 (1996)
• [Publications] K.Yamamoto et.al.: "Superiority of brain natriuretic peptide as a hormonal marker of ventricular systolic and diastolic dysfunction and ventricular hypertrophy." Hypertension. Vol.28. 988-994 (1996)
• [Publications] N.Tamaru.et.al.: "Two cardiac natriuretic peptide genes(atrial natriuretic peptide and brain natriuretic peptide)are organized in tandem in the mouse and human genomes." Journal of Mokecular Cellular Cardiology. Vol.28. 1811-1815 (1996)
• [Publications] M.Ishikawa et.al.: "cDNA cloning of rat cardiotrophin-1(CT-1) : augmented expression of CT-1 gene in ventricle of genetically hypertesive rats." Biochemical and Biophysical Research Communication. 219(2). 377-381 (1996)
• [Publications] T.wallen et.al.: "Brain natriuretic peptide predicts mortality i the elderly." Heart. (in press). (1997)
• [Publications] M.Yoshibayashi et.al.: "Brain ntriuretic peptide versus atrial ntriuretic peptide : Physiological and pathophysiological significance in children and adults." European Journal of Endocrinology. Vol.135. 265-268 (1996)