| Project/Area Number |
08557066
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
UCHIDA Shinichi (1997-1998) Tokyo Medical and Dental University, Instructor, 医学部, 助手 (50262184)
丸茂 文昭 (1996) 東京医科歯科大学, 医学部, 教授 (00050443)
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| Co-Investigator(Kenkyū-buntansha) |
伏見 清秀 東京医科歯科大学, 医学部, 助手 (50270913)
内田 信一 東京医科歯科大学, 医学部, 助手 (50262184)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1998: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1996: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Aquaporin / Water transport / Yeast / Kidney / Collecting duct / Membrane transport / Protein Structure / AQP2 / バゾプレシン / 利尿剤 / 尿濃縮機構 / 集合尿細管 |
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| Research Abstract |
In this research, intended to clarify structure-function relationship of kidney collecting duct water channel, AQP2, by using yeast expression system. The newly developved system used a yeast strain BJ3505 which lacks endogenous protease. Yeast cells were incubated at 30 degree, a-id intracellular vesicle fration was obtained by sucrose density gradient. Western blot analysis confirmed the selective expression of AQP2 proteins in this fraction. Osmotic water permeability (Pf) of these vesicles was determined by stoped flow method. Expression of AQP2 induced 22-fold increase of Pf, demonstrating the establishment of the suitable system for analysis of structure-function relationship of AQP2.
Using this system, we measured Pf of mutant AQP2s ; mutations were introduced along the aminoacid sequence. The results showed that when mutations were introduced between Loop C and Loop E, Pf was severely impaired. The amount AQP2 protein in the vesicles were not different among the mutants. Thus, we could determine the domain which is critically important for aquaous route of AQP2. We are currently narrowing this region 1r isolate critical residues, this information would be directly applicable in the development of inhibitors of membrane transport.
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