• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Specific cancer immunotherapy using tumor-rejection antigens of MAGE.

Research Project

Project/Area Number 08557074
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TSUYOSHI Akiyoshi  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,PROFESSOR, 生体防御医学研究所, 教授 (70038660)

Co-Investigator(Kenkyū-buntansha) YASUMOTO Masazumi  BIOTECHNOLOGY RESEARCH LABORATORIES,TAKARA SHUZO CO., LTD.RESEARCHER, バイオ研究所, 研究員
TAKESAKO Kazutoh  BIOTECHNOLOGY RESEARCH LABORATORIES,TAKARA SHUZO CO., LTD.VISE DIRECTOR, バイオ研究所, 副室長
TOH Yasushi  CLINICAL RESEARCH INSTITUTE,NATIONAL KYUSHU CANCER CENTER,DIVISION OF HEAD, 臨床研究部, 室長 (20217459)
YAMAGATA Motoyuki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,LECTURER, 生体防御医学研究所, 助手 (90294975)
HARAGUCHI Masaru  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,ASSISTANT PROFESSOR, 生体防御医学研究所, 講師 (40228531)
糠谷 育衛  宝酒造(株), バイオ研究所, 研究員
馬場 欽也  九州大学, 生体防御医学研究所, 助手 (30271117)
楠本 宏記  九州大学, 生体防御医学研究所, 助手 (00195447)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥18,400,000 (Direct Cost: ¥18,400,000)
Fiscal Year 1997: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1996: ¥11,900,000 (Direct Cost: ¥11,900,000)
Keywordstumor-rejection antigen / specific cancer immunotherapy / MAGE / antigenic peptide / cytotoxic Tlymphocyte / dendritic cell / cancer vaccine therapy / 細胞傷害性T細胞 / 特異的キラーT細胞 / ワクチン療法
Research Abstract

1. The expression of MAGE-1, -2, and-3 genes was relatively high in gastrointestinal carcinomas (40% in gastric carcinoma, 60% in esophageal carcinomas, and 60% in hepatocellular carcinomas). Eighty to 90% of these carcinomas expressed at least one of MAGE-1 to-12genes.
2. Using our newly developed, simplified method, MAGE peptide-specificcytotoxic T lymphocytes (CTL) that killed MAGE expressing tumor cells, as well as target cells pulsed with the peptide, could be induced from peripheral blood mononuclear cells (PBMC) of either healthy donors or cancer patients.
3. Because HLA-A24 is the most common allele in Japanese, MAGE-encoded peptides with a high binding affinity to HLA-A24 were defined based on the screening of sequences for the presence of MHC binding motifs. The immunogenicity of these pepitides was then tested by primary in vitro CTL induction using our simplified method. As the results, HLA-A24 restricted potential MAGE-1 and-3 peptides, IMPKAGLLI and NYKHCFPEI,have been identified.
4. Clinical trials using MAGE Peptides have been approved by ethics commitee of this institute. Therefore, patients with advanced gastrointestinal carcinoma have been treated with autologous dendritic cells pulsed with MAGE peptide as cancer vaccine. Following the treatment, one patient with advanced gastric carcinoma developed inflammatory change at tumor site of abdominal wall and specific CTL could be induced from PBMC of the patient. In a patient with recurrent carcinoma of the esophagus, metastatic tumor of cervial region has been regressed after two administration of the cancer vaccine. These results may suggest the effectiveness of this type of vaccine therapy using MAGE peptides.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tanaka F et al.: "Efficient induction of anti-tumor cytotoxic T lymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro." Cancer Immunol Immunother. 44. 21-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka F et al.: "Higher frequency of the expression of the MAGE gene family in human esophageal carcinoma." Int J Oncol. 10. 1113-1117 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujie T et al.: "Expression of MAGE and BAGE genes in Japanese breast carcinoma." Ann Oncol. 8. 369-372 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka F et al.: "Induction of antitumor cytotoxic T lymphocytes with a MAGE-3-encoded synthetic peptide presented by human leukocytes antigen-A24." Cancer Res. 57. 4465-4468 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li J et al.: "Expression of the MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujie et al.: "Induction of antitumor cytotoxic T lymphocytes from the peripheral blood mononuclear cells of cancer patients using HLA-A2-restricted MAGE-3 peptide in vitro." Clin Cancer Res. 3. 2425-2430 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hasegawa H et al.: "Expression spectrum of MAGE gene family members in colorectal carcinoma." Arch Pathol Lab Med. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] LiJ,et al.: "Expressiion of BAGE,GAGE,and MAGE genes in human gastric carcinoma." Clin Cancer Res. 2. 1619-1625 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mori M,et al.: "Expression of MAGE genes in human colorectal carcinoma." Ann Surg. 224. 183-188 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka F,et al.: "Efficient induciton of anti-tumor cytotoxic Tlymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro." Cancer Immunol Immunother. 44. 21-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka F,et al.: "Higher frequency of the expression of the MAGE gene family in human esophageal carcinoma." Int J Oncol. 10. 1113-1117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujie T,et al.: "Expression of MAGE and BAGE genes in Japanese breast carcinoma." Ann Oncol. 8. 369-372 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka F,et al.: "Induction of antitumor cytotoxic Tlymphocytes with a MAGE-3-encoded synthetic peptide presented by human leukocyte antigen-A24." Cancer Res. 57. 4465-4468 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] LiJ,et al.: "Expression of the MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fijie T,et al.: "Induction of antitumor cytotoxic Tlymphocytes from the peripheral blood mononuclear cells of cancer patients using HLA-A2-restricted MAGE-3 peptide in vitro." Clin Cancer Res. 3. 2425-2430 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hasegawa H,et al.: "Expression spectrum of MAGE gene family members in colorectal carcinoma." Arch Pathol Lab Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka,F.et al.: "Efficient induction of anti-tumor cytotoxic T lymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro." Cancer Immunol Immunother. 44. 21-26 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,F.et al.: "Higher frequency of the expession of the MAGE gene family in human esophageal carcinoma." Int J Oncol. 10. 1113-1117 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujie,T.et al.: "Expression of MAGE and BAGE genes in Japanese breast carcinoma." Ann Oncol. 8. 369-372 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka,F.et al.: "Induction of antitumor cytotoxic T lymphocytes with a MAGE-3-encoded synthetic peptide presented by human leukocytes antigen-A24." Cancer Res. 57. 4465-4468 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li,J.et al.: "Expression of the MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujie,T.et al.: "Induciton of antitumor cytotoxic T lymphocytes from the peripheral blood mononuclear cells of cancer patients using HLA-A2-restricted MAGE-3 peptide in vitro." Clin Cancer Res. 3. 2425-2430 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hasegawa,H.et al.: "Expression spectrum of MAGE gene family members in colorectal carcinoma." Arch Pathol Lab Med. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Li,J.: "Expression of BAGE,GAGE and MAGE genes in human gastric carcinoma." Clin. Cancer Res.2. 1619-1625 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mori,M.: "Expression of MAGE genes in human colorectal carcinoma." Ann.Surg.224. 183-188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka,F.: "Efficient induction of anti-tumor cytotoxic T lymphocytes from a healthy donor using HLA-A2-restricted MAGE-3 peptide in vitro." Cancer Immunol.Immunother.(in press).

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi