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Establishment of Helicobacter pylori infection models in rats and mice

Research Project

Project/Area Number 08557132
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biological pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

OKABE Susumu  Kyoto Pharmaceutical University Department of Applied Pharmacology, Professor, 薬学部, 教授 (90012624)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Satoru  Kyoto Pharmaceutical University Department of Applied Pharmacology, Research Ass, 薬学部, 助手 (20268098)
NISHINO Takeshi  Kyoto Pharmaceutical University Department of Microbiology, Professor, 薬学部, 教授 (50097838)
中野 妙  京都薬科大学, 薬学部, 副手 (70278448)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1996: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsHelicobacter pylori / infection / rat / mouse / 感染 / 胃潰瘍 / 除菌
Research Abstract

This study was designed to establish Helicobacter pylori (H.pylori) infection models in rats and mice. H.pylori of VacA-and CagA-positive strain (ATCC43504) was once orally inoculated to the animals. H.pylori infection was not observed in normal Donryu, Sprague-Dawley or Wistar rats. In contrast, the rats (Donryu strain) with gastric ulcers were infected with H.pylori. Gastric ulcers spontaneously healed, but H.pylori infection tended to impaire ulcer healing. The number of H.pylori in the stomach decreased with ulcer healing. Similarly, the rats (Sprague-Dawley strain) with HCl-induced mucosal lesions were infected with H.pylori. The number of H.pylori was associated with lesion area and decreased with the healing of the lesions. H.pylori infection did not affect the healing. On the other hand, in normal ddY mice, H.pylori persistently colonized the gastric mucosa for at least 26 weeks. The number of H.pylori was decreased by antibiotics such as amoxicillin and clarithromycin, and the antibiotics with proton pump inhibitors (omeprazole and lansoprazole) more profoundly reduced H.pylori viability in the stomach. H.pylori infection caused gastritis with inflammatory cell infiltration, but gastric pathology was mild, compared with those in H.pylori-infected humans and Mongolian gerbils. Even after H.pylori-infected mice were treated with aspirin, the pathology was not largely promoted. Gastric ulcers did not occur in H.pylori-infected mice.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 池田 恭子ほか: "慢性潰瘍を有するラットにおけるH.pylori感染" 実験潰瘍(Ulcer Research). 24. 191-193 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okabe, S, et al: "Influence of H.pylori infection on healing of acetic acid-induced gastric ulcers in rats." J.Gastroenterol.Hepatol. 12(Suppl). A143 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amagase, K.et al: "Effect of clarithromycin in Helicobacter pylori-infected rats with acetic acid ulcers." J.Gastroenterol.Hepatol.12(Suppl). A247 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Minamide, A.et al: "Helicobacter pylori colonization in rats with HCI-induced gastric mucosal lesions." J.Gastroenterol.Hepatol.12(Suppl). A210 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li, T.et al.: "Effects of clarithromycin,omeprazole and Ieminoprazole on gastric ulcer healing in Helicobacter pylori-infected rats." J.Gastroenterol.Hepatol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kyoko Ikeda, Yoshihiro Keto, Susumu Okabe: "Helicobacter pylori infection in rats with or without acetic acid gastric ulcers." Ulser Research. Vol.24. 191-193 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Susumu Okabe, Tie Li: "Influence of H.pylori infection on healing of acetic acid-induced gastric ulcers in rats." J.Gastoenterol.Hepatol. Vol.12 (Suppl.). A143 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kikuko Amagase, Akinobu Minamide, Tie Li, Kyoko Ikeda, Susumu Okabe: "Effect of clarithromycin in Helicobacter pylori-infected rats with acetic acid ulcers." J.Gastoenterol.Hepatol. Vol.12 (Suppl.). A247 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akinobu Minamide, Yoshihiro Keto, Norikazu Takagi, Susumu Okabe: "Helicobacter pylori colonization in rats with HCl-induced gastric mucosal lesions." J.Gastoenterol.Hepatol. Vol.12 (Suppl.). A210 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tie Li, Susumu Okabe: "Effects of clarithromycin, omeprazole and leminoprazole on gastric ulcer healing in Helicobacter pylori-infected rats." J.Gastoenterol.Hepatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 池田恭子ほか: "慢性潰瘍を有するラットにおけるH.pylori感染" 実験潰瘍(Ulcer Research). 24. 191-193 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okabe,S.et al.: "Influence of H.pylori intection on healing of acetic acid-induced gastric ulcers in rats." J.Gastroenterol.Hepatol.12(Suppl). A143 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Amagase,K.et al.: "Effect of clarithromycin in Helicobacter pylori-infected rats with acetic acid ulcers." J.Gastroenterol.Hepatol.12(Suppl). A247 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Minamide,A.et al.: "Helicobacter pylori colonization in rats with HCl-induced gastric mucosal lestions." J.Gastroenterol.Hepatol.12(Suppl). A210 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li,T.et al.: "Effects of clarithromycin,omeprazole and leminoprazole on gastric ulcer healing in Helicobacter pylori-infected rats." J.Gastroenterol.Hepatol.(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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