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Molecular cloning related to skeletal or muscle atrophy under microgravity

Research Project

Project/Area Number 08557151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Laboratory medicine
Research InstitutionKagoshima University

Principal Investigator

KITAJIMA Isao  Kagoshima University Faculty of Medicine, Associate Professor, 医学部, 助教授 (50214797)

Co-Investigator(Kenkyū-buntansha) HANYU Naojiro  Tokuyama, Co.Ltd.Chief.Reseacher, 基礎研究センター, 主任研究員
OHIRA Yoshinobu  Natl.Inst.Fitness and Sports, Kanoya, Professor, 体育学部, 教授 (50185378)
SEMBA Ichiro  Kagoshima University Faculty of Dent, Associate Professor, 歯学部, 助教授 (60145505)
MARUYAMA Ikuro  Kagoshima University Faculty of Medicine Professor, 医学部, 教授 (20082282)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsSpace-flight / rat / bone atrophy / remodeling / microgravity / metal composition / mRNA / cloning / 宇宙飛行 / 傍背柱筋 / 萎縮 / ストレス蛋白 / MEF-2 / 骨モデリング / 椎体骨 / Diffential display
Research Abstract

The effect of 14 days of flight on the vertebrae of rapidly growing rats was studied. No significant difference (p>0.05) was observed between flight rats and ground controls with regard to mechanical hardness or bone mineral density. however, histological examination revealed irregular thickening of the endosteal surface of cortical bones in flight rats, whereas it was uniform in the ground controls. The relative area of lamellar bone showed a significant reduction (p<0.001) in the flight rats. These findings suggest that structural disturbances were due to retardation of endosteal modeling and remodeling. Next, the effects of 14 days of space-flight on beta-adrenoceptor (beta-AR), mitochondrial enzyme activities, and fiber type composition were studied in plantaris muscles. The maximum binding capacity of beta-AR (B max) was significantly lower (>29%) after flight, and the recovery was not complete after 9 days in a 1-G environment. The activity of succinate dehydrogenase (SDH) was measured in muscle samples <5 hr after flight and were found to normalize during 9 days of recovery. It is suggested that the space-flight-induced decrease of B max of beta-AR in plantaris was accompanied by a decreased activity of a mitochondrial inner-membrane enzyme, SDH.Finally, the expression of mRNA in paraspinal muscles between space-flight rats and ground controls was compared using mRNA differential display method in order to identify molecules related to muscle atrophy under microgravity. We found that 70 kDa heat-shock protein (HSP70) and its retated gene, TCP-1, were makedly elevated during 14 days of space-flight, while genes involved in mitochondrial metabolism (mitochondrial adenosine nuclease translocator, mitochondrial cytochrome oxidase subunits), aldolase A and myocyte-specific enhancer binding factor (MEF) were significantly decreased. Some new genes related to muscle atrophy were also detected and we studied the biological functions of these molecules.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Isao Kitajima, Naohiro Hanyu et al.: "Ribozymc-based gene cleavage approach to chronic arthritis associated with human T-cell leukemia virus typeI (HTLV-I) :" Arthritis & Rheumatism. 40. 2118-2127 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isao Kitajima, Naohiro Hanyu, et al: "Efficient transfer of synthetic ribozymes into cells using hemagglutinating virus of Japan (HVJ) -cationic liposomes." J.Biol.Chem.272. 27099-27106 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Gen Yamada, Isao Kitajima, et al: "Metal composition and histological analysis of the space flight rat bones" Life Science. 60. 635-642 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jun-Ichiro Nishi Isao Kitajima, et al.: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B :" J.Immunol.158. 247-254 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima, I., Senba, I.et al: "growth disfurbance in vertebral bones of rapidly growing rato following 14 days of spaceflight" Journal of Applied physiology. 81(1). 156-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohira, Y., Yasui, W, et al: "Spaceflight effects on β-adrenoceptor and metabolic properties in rat plantarls" Journal of Applied Physiology. 81(1). 152-155 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ikuro Maruyama, Isao Kitajima, et al: "Thrombin activates NF-kB through thrombin receptor" Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIER,Amsterdam,260 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Kitajima, N.Hanyu, K.Kawahara, Y.Soejima, T.Kubo, R.Yamada, Y.Kaneda, I.Maruyama: "Ribozyme-based gene cleavage approach to chronic arthritis associated with human T-cell leukemia virus type I (HTLV-I) : Induction of apoptosis in synoviocytes by ablation of HTLV-I Tax protein." Arthritis.Rheum.40. 2118-2127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Kitajima, N.Hanyu, Y.Soejima, R.Hirano, S.Arahira, S.Yamaoka, R.Yamada, I.Maruyama, Y.Kaneda: "Efficient transfer of synthetic ribozymes into cells using hemagglutinating virus of Japan (HVJ)-cationicl iposomes. Application for ribozymes that target Human T-cell leukemia virus type I tax/rex mRNA." J.Biol.Chem.272. 27099-27106 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Yamada, K.Sugimura, S.Nakamura, M-O.Yamada, Y.Okazaki, I.Maruyama, I.Kitajima, T.Minami: "Metal comp[osition and histological analysis of the space flight rat bones." Life Science. 60. 635-642 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Nishi, S.Kanekura, S.Takei, I.Kitajima, T.Nakajima, M.R.Wahid, K.Matsuda, M.Yoshinaga, I.Maruyama, K.Miyata: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B : The Diminant epitope region recognized by intravenous IgG." J.Immounol.158. 247-254 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Kitajima, I.Senba, T.Noikura, K.Kawano, Y.Iwashita, I.Takasaki, I.Maruyama: "Growth disturbance in vertebral bones of rapidly growing 14 days of spaceflight." J.Appl.Physiol.81. 156-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Ohira, W.Yasui, F.Kariya, T.Tanaka, I.Kitajima, I.Muruyama, S.Nagaoka.C.Sekiguchi, W.E.Hinds: "Spaceflight effects on beta-adrenoreceptor and metabolic poperties in rat plantaris." J.Appl.Physiol.81. 152-155 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ikuro Maruyama, Isao Kitajima, et al: Thrombin activalcs NF-kB through thrombin receptor. Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIRE,Amsterdam, 161-167 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Isao Kitajima Naohiro Hanyu,et al: "Ribozyme-based gene cleavage approach to chronic arthritis associated with human T-cell leukemia virustype I(HTLV-I):" Arthritis & Rheumatism. 40. 2118-2127 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Isao Kitajima Naohiro Hanyu,et al.: "Efficient transfer of synthetic ribozymes into cells using hemagglutinating virus of Japan(HVJ)-cationic liposomes." J.Biol.Chem.272. 27099-27106 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jun-Ichiro Nishi Isao Kitajima: "B cell epitope mapping of the bacterial superantigen staphylococcal enterotoxin B:" J.Immunol.158. 247-254 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Gen Yamada, Isao Kitajima, et al.: "Metal composition and histological analysis of the space flight rat bones" Life Science. 60. 635-642 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kimiyoshi Arimura Isao Kitajima, et al: "Antibodies to potassium channel of PC12 in serum of Isaacs'syndrome:" Muscle & Nurve. 20(3). 299-305 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mika Okamoto Isao Kitajima, et al.: "HIV-1-infected myclomonocytic cells are resistant to Fas-mediated apoptosis:" Medical Microbiology and Immunology. 186. 11-17 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 北島 勲: "エンドトキシンのシグナル伝達(DICology)" メデイカル レビュー社, 145-153 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikuro Maruyama Isao Kitajima, et al.: "Thrombin activates NF-kB through thrombin receptor" Recent Progress in Blood Coagulation and Fibrinolysis ELSEVIER,Amsterdam., 161-167 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitajima,I.,et al..: "Growth disturbance in vertebiral bones of rapidly growing rats following 14 days of spaceflight." J.Appl.Physiol.81(1). 156-163 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohira,Y.,et al.: "Spaceflight effects on β-adrenoreceptor and metabolic propeties in rat plantars." J.Appl.Physiol.81(1). 152-155 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitajima,I.,et al.: "Induction of apoptosis in murine clonal osteoblasts expressed by human T-cell leukemia virus type I tax by NF-kB and TNE-α." J.Bone.Miner.Res.11. 200-210 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitajima,I.,et al.: "Ceramide-induced nuclear translocation of NF-kB is a potential mediator of the apoptotic response to TNF-a in murine clonal osteoblasts." BONE. 19(3). 305-306 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitajima,I.,et al.: "Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I tax expressing PC12" J.Cell.Sci.109. 609-617 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishidou,Y.,et al.: "Enhanced expression of type 1 receptor for bone morphogene proteins during bone formation." J.Bone.Miner.Res.10. 1651-1659 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitajima,I.,et al.: "Therapeutic application of antisense oligodeoxynucleotides to inflammation" Kyushu University Press, 171 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北島 勲: "サイトカイン,アンチセンス療法" メディカルレビュー社, 255 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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