| Project/Area Number |
08558058
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
ONO Yoshiro OKAYAMA UNIV.DEPT.ENVIRONMENTAL & CIVIL ENGG., ASSOCIATE PROFESSOR, 環境理工学部, 助教授 (50152541)
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| Co-Investigator(Kenkyū-buntansha) |
SOMIYA Isao KYOTO UNIV.DEPT.ENVIRONMENTAL ENGG., PROFESSOR, 大学院・工学研究科, 教授 (60025947)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1996: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | umu test / flow injection / bacteria assay |
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| Research Abstract |
Various bioassays have been applied to evaluate safety of environmental water and treated water. The umu-test is a method for the detection of genotoxicity by using SOS function of Salmonella typhimurium.
The protocol of the umu-test in devided into 5 phases, (1) pre-incubation of test strain, (2) addition of samples, (3) induction of umuC gene, (4) Beta-galactosidase enzymati reaction and (5) measurement of absorption in a spectrophotometry and calculation of the activity.
In usual procedure, all phases are performed by batch type reactors, using test tubes.
In this study, we tried to developed the device of flow-injection style to perform the umu test automatically. The culture was flowed through a tube and added samples by using a rotary pump. The various conditions were investigated and modified for detection of genotoxocity of AF-2.
Even after 24hr of incubation under control, test strain showed activity. Within 1.5hrs, the test strain was most active at 36 deg.C and longer than 2.0hrs, it was so at 37 deg.C.At each phases some more conditions have been investigated, and we suggest that new devise of flow-injection style could be effective to screen or monitor the genotoxicity of environmental water.
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