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Development of purification methods for solids contaminated by hydrocarbon from mineral oils.

Research Project

Project/Area Number 08558065
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 環境保全
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TERASHIMA Yutaka  Kyoto University Faculty of Engineering Professor, 工学研究科, 教授 (50019717)

Co-Investigator(Kenkyū-buntansha) MIYAKE Syunsaku  Organo Co.Ltd Division of Earth Environment Head of the Division, 地球環境部, 部長(研究職)
KOSHIKAWA Hiromoto  Kyoto University Faculty of Engineering Instructor, 工学研究所, 助手 (70273480)
SHIMIZU Yoshihisa  Kyoto University Faculty of Engineering Associate Professor, 工学研究科, 助教授 (20226260)
尾崎 博明  京都大学, 工学研究科, 助教授 (40135520)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1996: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordswhite rot fungus / Phanerochaete chrysosporium / ligninolytic enzymes / mineral oil / degradation / treatment / 白色腐朽菌 / Phanerochate chrysosporium / 土壌・地下水汚染 / A重油 / 汚染土壌 / 廃水処理 / 固定化 / 土壌汚染 / 汚染機構 / 重油 / 油分汚染 / 土壌浄化 / 油分分解菌 / 重油分析法 / 重油画分
Research Abstract

The environmental factors influencing the growth characterization of the white rot fungus P.chrysosporium, the degradation of several kinds of recalcitrant substance, and the activities of extracellular enzymes were investigated by batch culture experiments. The possibility of applying this fungus for the removal of the recalcitrant substance from contaminated soil were also evaluated.
Main results were summarized as follows ;
1) The temperature and pH effect the growth of the P.chrysosporium significantly, and the presence of vitamins especially, thiamin was necessary for its growth.
2) LiP activity appeared after glucose depletion, MnP appeared after glucose or nitrogen depletion. Higher LiP activity was obtained by increasing the nitrogen concentration.
3) Amino acid, NO_3^-, NH can be used as nitrogen source of P.chrysosporium, but NH_4^+ was proved to be the best.
4) The polyurethane foam was found to be the best carrier of P.chrysosporium according to the results of ligninase production. And P.chrysosporium can grow without addition of medium when immobilized on aspen chips.
5) LiP and MnP production was obtained in the batch reactor using P.chrysosporium immobilized on polyurethane foam.
6) 1,000mg/L of heavy oil A was degraded by the extracellular enzymes in liquid medium.
7) P.chrysosporium was inoculated soils contaminated by heavy oil A, the concentration of oil in soil was measured. The oil was degraded, but the rate was slower than in the case of liquid medium.
These results suggest the possibility of decontamination of soils by P.chrysosporium.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 寺島 泰: "白色腐朽菌Phanerochaete chrysosporiumの固定化とその廃水及び汚染土壌の処理への応用" 環境工学研究論文集. 33. 195-203 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 寺島 泰: "Activites of Ligninolytic Enzymes of the White Rot Fungus, Phanerochate Chrysosporium and Its Recalcitrant Substance Degradability" Water Science and Technology. 34. 69-78 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 寺島 泰: "白色腐朽菌の固定化とアゾ染料の脱色" 土木学会第51回年次学術講演会. 382-383 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 寺島 泰: "白色不朽菌のリグニン分解酵素活性に対する培地組成の影響" 土木学会第52回年次学術講演会講演概要集. 382-383 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terashima, T.et al.: "Immobilization of White Rot Fungus, Phanerochaete chrysosporium and its applications to wastewater and contaminated soil treatment" Proceedings of Environmental Engineering Research. Vol.33. 195-203 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terashima, T.et al.: "Activities of Ligninolytic Enzymes of the White Rot Fungus, Phanerochaete chrysosporium and its Recalcitrant Substance Degradability" Water Science & Technology. Vol.34. 69-78 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terashima, T.et al.: "Immobilization of White Rot Fungus and Decolorization of Azo Dyes" Proceedings of the 51th Annual Conference of the Japan Society of Civil Engineers. 382-383 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Terashima, T.et al.: "Factors of medium for Ligninolytic Enzymes Activities from White Rot Fungus" Proceedings of the 52th Annual Conference of the Japan Society of Civil Engineers. 382-383 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 尾崎 博明: "白色腐朽菌Phanerochaete chrysosporiumの固定化とその廃水及び汚染土壌の処理への応用" 環境工学論文集. 33. 195-203 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 井上 直子: "白色腐朽菌のリグニン分解酵素活性に対する培地組成の影響" 土木学会第52回年次学術講演会講演概要集. 382-383 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 尾崎 博明: "白色腐朽菌Phanerochaete chrysosporiumの固定化とその廃水及び汚染土壌の処理への応用" 環境工学論文集. 33. 195-203 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Ozaki: "Degradation of Petroleum Oil by white Rot Fungus,P.chrysosporium" Proc.of Second International Congress and Environmental Geotechnies. II. 1035-1038 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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