| Project/Area Number |
08558069
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Structural biochemistry
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TANAKA Isao Hokkaido Univ., Graduate School of Sciences, Professor, 大学院理学研究科, 教授 (70093052)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Mamoru Institute of Materials Structure Science, Assistant Professor, 物質構造科学研究所・放射線研究施設, 助手 (40280507)
NAKAGAWA Atsushi Hokkaido Univ., Graduate School of Sciences, Associate Professor, 大学院理学研究科, 助教授 (20188890)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1997: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Protein structure analysis / Multiwavelength anomalous diffraction method / Selenomethionine / 多波長異状分散法 |
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| Research Abstract |
For the last 40 years, almost all new protein structures have been solved by the multiple isomorphous replacement (MIR) method. To apply this method for the phase problem we need to prepare good heavy-atom derivatives, which is one of the most timeconsuming process for the protein structure analysis, because it entails the trial-and-error experiments. Recently, an alternative approach has been devised for de novo phasing of protein crystals. This is the multiwavelengh anomalous diffraction (MAD) method. Especially important is the use of selenium atom for the anomalous scatterer, since it is easily incorporated into protein crystal by the protein engineering as a form of selenomethionine (Se-Met). However, there are still several difficulties in applying the method to the actual phase problems, especially at the process of deciphering the Patterson map, mainly because of the weak signal from the anomalous scatterer. In this project we applied this method to the analyzes of several new proteins for further improvement of the method and showed that this method is very useful.
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