| Project/Area Number |
08558085
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
神経・脳内生理学
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| Research Institution | KAWASAKI MEDICAL SCHOOL |
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Principal Investigator |
TSUJIOKA Katsuhiko Dept.Physiology, KAWASAKI MEDICAL SCHOOL Professor, 医学部, 教授 (30163801)
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| Co-Investigator(Kenkyū-buntansha) |
OGASAWARA Yasuo Dept.ME,KAWASAKI MEDICAL SCHOOL Associate Professor, 医学部, 助教授 (10152365)
KAJIYA Fumihiko Dept.ME,KAWASAKI MEDICAL SCHOOL Professor, 医学部, 教授 (70029114)
KOGA Tomoshige Dept.Physiology, KAWASAKI MEDICAL SCHOOL Assistant Professor, 医学部, 助手 (50186649)
YAMANE Masanobu Kawasaki College, Professor, 第二看護科, 教授 (90069071)
FUKUDA Hiroyuki Dept.Physiology, KAWASAKI MEDICAL SCHOOL Associate Professor, 医学部, 助教授 (20069016)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1996: ¥10,700,000 (Direct Cost: ¥10,700,000)
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| Keywords | cerebral cortex / blood flow regulation / arteriole / vessel size / needle probe / intravital microscopy / CCD / 脳血流 / 細静脈 / CCD生体顕微鏡 |
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| Research Abstract |
Analysis of cerebral microcirculation is of importance, as higher function of cerebral cortex is completely dependent on blood flow. The purpose of this study was to develop in vivo visualization system for microcirculation and neural activity of cerebral cortex by using needle probe CCD intravital microscope. During the first year we focused on visualization of arteriole and venule and developed a new system, and checked feasibility of the system. This system is composed of a needle-probe with relay lens, three-plate CCD and high difinition monitor. Time and space resolution of this system is 33ms and 2mum, respectively, and distortion of image was not found. In anesthetized dogs, we could clearly observe pial arteriole and venule and movement of red blood cell. Furthermore, we could observe microvessels within cortex by insertion of the needle-probe.
In the next year, we investigated the mechanism (s) of regulation of cerebral blood flow. Inhalation of 10% CO_2, intracarotid injection of nitrol or adenosine remarkably increased carotid blood flow. In accordance with this increase, pial arteriole remarkably dilated. Relatively smaller arteriole (diameter<75mum) dilated much, but relatively larger arteriole (diameter*75mum) did not dilate significantly. In conclusion local cerebral blood flow is regulated mainly by relatevely smaller arteriole (<75mum). This mechamism may be beneficial for fine regulation of local cerebral blood flow.
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