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Development of visualization system for neural activity and microcirculation of cerebral cortex.

Research Project

Project/Area Number 08558085
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 神経・脳内生理学
Research InstitutionKAWASAKI MEDICAL SCHOOL

Principal Investigator

TSUJIOKA Katsuhiko  Dept.Physiology, KAWASAKI MEDICAL SCHOOL Professor, 医学部, 教授 (30163801)

Co-Investigator(Kenkyū-buntansha) OGASAWARA Yasuo  Dept.ME,KAWASAKI MEDICAL SCHOOL Associate Professor, 医学部, 助教授 (10152365)
KAJIYA Fumihiko  Dept.ME,KAWASAKI MEDICAL SCHOOL Professor, 医学部, 教授 (70029114)
KOGA Tomoshige  Dept.Physiology, KAWASAKI MEDICAL SCHOOL Assistant Professor, 医学部, 助手 (50186649)
YAMANE Masanobu  Kawasaki College, Professor, 第二看護科, 教授 (90069071)
FUKUDA Hiroyuki  Dept.Physiology, KAWASAKI MEDICAL SCHOOL Associate Professor, 医学部, 助教授 (20069016)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 1997: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1996: ¥10,700,000 (Direct Cost: ¥10,700,000)
Keywordscerebral cortex / blood flow regulation / arteriole / vessel size / needle probe / intravital microscopy / CCD / 脳血流 / 細静脈 / CCD生体顕微鏡
Research Abstract

Analysis of cerebral microcirculation is of importance, as higher function of cerebral cortex is completely dependent on blood flow. The purpose of this study was to develop in vivo visualization system for microcirculation and neural activity of cerebral cortex by using needle probe CCD intravital microscope. During the first year we focused on visualization of arteriole and venule and developed a new system, and checked feasibility of the system. This system is composed of a needle-probe with relay lens, three-plate CCD and high difinition monitor. Time and space resolution of this system is 33ms and 2mum, respectively, and distortion of image was not found. In anesthetized dogs, we could clearly observe pial arteriole and venule and movement of red blood cell. Furthermore, we could observe microvessels within cortex by insertion of the needle-probe.
In the next year, we investigated the mechanism (s) of regulation of cerebral blood flow. Inhalation of 10% CO_2, intracarotid injection of nitrol or adenosine remarkably increased carotid blood flow. In accordance with this increase, pial arteriole remarkably dilated. Relatively smaller arteriole (diameter<75mum) dilated much, but relatively larger arteriole (diameter*75mum) did not dilate significantly. In conclusion local cerebral blood flow is regulated mainly by relatevely smaller arteriole (<75mum). This mechamism may be beneficial for fine regulation of local cerebral blood flow.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(23 results)

All Other

All Publications (23 results)

  • [Publications] E. Toyota: "Evaluation of intramyocardial coronary blood flow waveform during intraaortic balloon pumping in the absence or presence of coronary stenosis" Artificial Organs. 20 (2). 166-168 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T. Matsumoto: "Microheterogeneity of myocardial blood flow within the rabbit left ventricle during the normoxic and hypoxic states." American Journal of Physiology. 270. H435-H441 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S. A. Jones: "In vivo measurements of blood flow velocity profiles in canine ilio-femoral anastomotic bypass grafts." Journal of Biomechanical Engineering. 119. 30-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K. Morita: "α-Adrenergic vasoconstriction reduces systolic retrograde coronary blood flow." American Journal of Physiology. 273. H2746-H2755 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K. Shimmyo: "In vivo observation of cerebral microcirculation by CCD videomicroscope with a gneedle probe." XXXIII International Congress of Physiological Sciences. IUPS. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y. Saitou: "A needle probe CCD videomicroscope for in vivo observation of microceiculation of the brain." Medical and Biological Engineering and Computing. 35. H64-PS1.08 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Toyota, S.Lu, A.Kimura, O.Hiramatsu, H.Tachibana, Y.Chiba, M.Goto, Y.Ogasawara, K.Tsujioka, F.Kajiya: "Evaluation of intramyocardial coronary blood flow waveform during intraaortic balloon pumping in the absence or presence of coronary stenosis." Artificial Organs. 20 (2). 166-168 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsumoto, M.Goto, H.Tachibana, Y.Ogasawara, K.Tsujioka, F.Kajiya: "Microheterogeneity of myocardial blood flow within the rabbit left ventricle during the normoxic and hypoxic states." American Journal of Physiology. 270. H435-H441 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.A.Jones, F.Loth, D.P.Giddens, I.Morita, F.Kajiya, O.Hiramatsu, Y.Ogasawara, K.Tsujioka, C.K.Zarins: "In vivo measurements of blood flow velocity profiles in canine iliofemoral anastomotic bypass grafts." Journal of Biomechanical Engineering. 119. 30-38 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Morita, H.Mori, K.Tsujioka, A.Kimura, Y.Ogasawara, M.Goto, O.Hiramatsu, F.Kajiya, E.O.Feigl: "alpha-Adrenergic vasoconstriction reduces systolic retrograde coronary blood flow." American Journal of Physiology. 273. H2746-H2755 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shimmyo, Y.Saito, T.Koga, M.Yamane, H.Fukuda, K.Tsujioka, T.Ymamoto, Y.Ogasawara, F.Kajiya: "In vivo observation of cerebral microcirculation by CCD videomicroscope with a needle probe." XXXIII International Congress of Physiological Sciences IUPS. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitou, K.Shimmyo, T.Koga, M.Yamane, H.Fukuda, K.Tsujioka, et.al.: "A needle probe CCD videomicroscope for in vivo observation of microceiculation of the brain." Medical and Biological Engineering and computing. 35, supplement 2. H64-PS1.08 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Toyota: "Evaluation of intramyocardial coronary blood flow waveform during intraaortic balloon pumping in the absence or presence of coronary stenosis" Artficial Organs. 20(2). 166-168 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Matsumoto: "Microheterogeneity of myocardial blood flow within the rabbit left ventricle during the normoxic and hypoxic states." American Journal of Physiology. 270. H435-H441 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.A.Jones: "In vivo measurements of blood flow velocity profiles in canine ilio-femoral anastomotic bypass grafts." JournalofBiomechanical Engineering. 119. 30-38 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Morita: "α-Adrenergic vasoconstriction reduces systolic retrograde coronary blood flow." American Journal of Physiology. 273. H2746-H2755 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shimmyo: "In vivo observation of cerebral microcirculation by CCD videomicroscope with a needle probe." XXXIII International Congress of Physiological Sciences. IUPS. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Saitou: "A needle probe CCD videomicroscope for in vivo observation of microceiculation of the brain." MedicalandBiologicalEngineeringand computing. 35. H64-PS1.08 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 斎藤靖浩: "ニードルプローブ型CCD生体顕微鏡による脳微小血管の観察" 第74回生理学会大会予稿集. 100- (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新名健治: "ニードルプローブ型CCD生体顕微鏡による脳微小循環の生体内計測" 医用工学と生体工学. 35特別号. (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeshi Matumoto: "Microheterogeneity of myocardial blood flow in rabbit hearts during normoxic and hypoxic states." American Jounal of Physiology. 270. H435-H441 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 平松修: "血流と微小血管可視化による交感神経α受容体を介する血管収縮の心筋内膜側心筋灌流に利する機序の解析" 医用電子と生体工学. 34特別号. 187- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山本徳則: "生体ヒト腎vasculo-tubular microcirculationの可視化" 医用電子と生体工学. 34特別号. 88- (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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