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Radiation Effects on Hybrid-Type Medical Materials

Research Project

Project/Area Number 08558100
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka Prefecture University

Principal Investigator

HAYASHI Toshio  Osaka Prefecture University ; Research Institute for Advanced Science and Technology ; Professor, 先端科学研究所, 教授 (90026089)

Co-Investigator(Kenkyū-buntansha) FURUTA Masakazu  Osaka Prefecture University ; Research Institute for Advanced Science and Techno, 先端科学研究所, 助手 (40181458)
FUJITA Shinichi  Osaka Prefecture University ; Research Institute for Advanced Science and Techno, 先端科学研究所, 教授 (60100210)
YONEZAWA Siro  Osaka Prefecture University ; Research Institute for Advanced Science and Techno, 先端科学研究所, 教授 (80090463)
OKA Masahito  Osaka Prefecture University ; Research Institute for Advanced Science and Techno, 先端科学研究所, 助教授 (70203966)
ARAI Motoo  Osaka Prefecture University ; Faculty of Agriculture ; Professor, 農学部, 教授 (80081537)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsHybrid-Type Medical Materials / Radiation Effects / Sterilization by Radiation / Immobilized Enzymes / gamma-ray Radiation / Electron Beam / Bacteria of Index for Sterilization / Destruction of Materials / 放射線減菌
Research Abstract

During the course of the development of biomedical materials, sterilization is essential since a material is contaminated with bacteria. Therefore, the polymeric material must be capable of being sterilized without detriment. Sterilization techniques are categorized as physical or chemical ones. Physical methods include dry heat, steam, and ionizing radiation. Chemical sterilization methods by using ethylene oxide is also used widely. Unfortunately, conventional autoclaving method cannot be used for biomimetic materials because they contain heat-labile bioactive components such as proteins. Ethylene oxide gas fumigation causes a health problem by traces of toxic and carcinogenic gas remaining in the material after treatment. Ionizing radiations are able to kill bacteria without including neither heat nor substances affecting the quality of the materials and our health. Thus the irradiation sterilization, which has already been applied to the medical supplies, should be the final candid … More ate for sterilization of the hybrid-type biomedical materials containing proteins. To apply commercial irradiation sterilization technique to the protein-containing hybrid-type biomadical materials, it is necessary to examine radiation torelance of proteins as well as other components at a standard doze of 25kGy generally used for commercial-scale irradiation. There are very few data available for these purpse until now.
In this project, to establish irradiation sterilization method for hybrid-type biomedical materials containing bioactive molecules together with artificial polymers, we examined radiation tolerance with 6OCo gamma-rays. We selected enzymes as a model of proteins. Furthermore, we designed various types of-biomedical polymers as well as hybrid-type materials for biomedical applications. Finally, it is concluded that the commercial irradiation sterilization method is applica ble to decontamination of enzymes under an appropriate condition such as dry or frozen state. Although radiation inactivation of enzymes in the aqueous solution was occurred, it could be depressed by control of the irradiation conditions. It is also concluded that the immobilized enzymesare much more stable than free ones for irradiation. These findings suggest that the irradiation sterilization will be useful for hybrid-type biomedical materials in future. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] 古田雅一、大橋 勲、岡 勝仁、林 壽郎: "滅菌を目的とした放射線照射に対するパパイン酵素の感受性評価" 生体材料. 17(1). 17-23 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 古田雅一、大橋 勲、岡 勝仁、林 壽郎: "滅菌を目的とした放射線照射による加水分解酵素の特性評価" 食品照射. 33. 41-46 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Nishimoto,S.Morimoto,and S.Fujita: "Hydroxyl radical-induced crosslinking of thymine and lysin: Identification of the primary structure and mechanism" Bioorg.Medic.Chem.Letters. 8. 865-87〓 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] H.Seo,K.Itoyama,T.Hayashi,et al.: "Spacer effects on enzymatic activity of bromelain immobilized onto porous chitosan beads" Eur.Polym.J.34(7). 917-922 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Nishidome,H.Mihara,M.Oka,T.Hayashi,et al.: "Structure and property of model peptides of proline/arginine-rich region in Bactenecin 5" J.Peptide Res.51. 337-345 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Takada,T.Kawaguchi,J.Sumitani,and M.Arai: "Cloning,nucleotide sequencing and transriptional analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase I." J.Ferment.Bioeng.85. 1-9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Arai,G.Takada,T.Kawaguchi,J.Sumitani: "Biochemical Principles and Mechanism of Biosynthesis and Degradation of Polymers" (ed.A.Steibuechel) Wiley-VCH,Germany, 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyachi, Y.Jokei, K.Oka, M.and Hayashi, T.: "Enzymatic Hydrolysis of Copoly (N-hydroxypropyl-L-glutamine/L-leucine) Hydrogels in vitro" J.Biomat.Sci., Polym.Ed.7 (9). 805-816 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida, Y.Makino, K.Ito, T.Yamakawa, Y.and Hayashi, T.: "Physical and Biodegradation Properties of A-B-A Type Block Copolymer Mem-branes Consisting of Poly (N-hydroxyalkyl-L-glutamine) as the A Component and Polybutadiene as the B Component" Eur.Polym.J.32 (7). 877-881 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoda, R.Komatsuzaki, S.and Hayashi, T.: "Membrane Properties of of A-B-A Type Block Coplymer Membranes Consisting of Poly (gamma-benzyl-L-glutamate) as the A Component and Polyisoprene as the B Component" Eur.Polym.J.32 (2). 233-237 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirano, Y.Madenokoji, K.Nakajima, A.Oka, M.Hayashi, T.: "Synthesis and Evaluation of Cell-attachment Activity of RGDS-related Molecules" Trans.Mater.Res.Soc., Japan. 20. 9-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa, M.Misonoh, J.and Hosokawa, Y.: "Two Types of X-ray-induced Radioresistance in mice : Pre sence of 4 dose Ranges with Distinct Biological Effects" Nutation Res.358. 237-243 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujita, S.Horii, H.Taniguchi, R.Lakshimi, S.Renganathan, R.: "Pulse Radiolytic Investigations on the Reaction of the 6-yl Radicals of Uracils with Cu(II)-Amino Acid Complexes" Radiat.Phys.Chem.48(5). 643-649 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa, M.Matsuda, Y.and Nishiyama, F.: "PIXE Analysis of the Serum of Mice Acquired Radioresistance after Small Dose of X-ray Irradiation" Intern.J.PIXE. 6(3). 523-530 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayashi, T.Oka, M.and Miyachi, Y.: "Molecular Design of Biodegradable Copolypeptide Membranes" Macromol.Symp.123. 163-175 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murai, T.Ueda, M.and Arai, M.: "Genetic Immobilization of Cellulase on the Cell Surface of Saccharomyces cerevisiae" Appl.Mictob.Biotechnol.48. 499-503 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda, Y.Yonezawa, M.and Nishiyama, F.: "PIXE Analysis of Serum of Mice Acquired Radioresistance after X-ray Irradiation of Small Dose (II)" Intern.J.PIXE. 7 (3). 233-240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo, H.Itoyama, K.Morimoto, K.Oka, M.Hayashi, T.: "Spacer Effects on Enzymatic Activity of Bromelain Immobilized onto Porous Chitosan Beads" Eur.Polym.J.34 (7). 917-922 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamada, Y.Murata, M.Makino, K.Yoshida, Y.and Hayashi, T.: "Anticoagulant Effects of Sulfonated Polyisoprene" Biomaterial.19. 745-750 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murai, T.Ueda, M.Kawaguchi, T.Arai, M.and Tanaka, A.: "Assimilation by a Cellooligosaccharides by Cell Surface Engineeried Yeast Expressing Beta-Glucosidase and Carboxymethylcellulase from Aspergillus aculeratus" Appl.Environ.Microbiol.64. 4857-4861 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takada, G.Kawaguchi, T.Sumitani, J.and Arai, M.: "Cloning, Nucleotide Sequencing and Transriptional Analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase 1 (cbhl) Gene" J.Ferment.Bioeng.85. 1-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawaguchi, T.Ikeuchi, Y.Tsutsumi, N.Kan, A.Sumitani, J.and Arai, M.: "Cloning and Nucleotide Sequencing and Expression of the Clostridium thermocellum Cellodextrin Phosphorylase Gene and Its Application to Synthesis of Crllulase Inhibitor" J.Ferment.Bioeng.85. 144-149 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishimoto, S.Morimoto, S.and Fujita, S.: "Hydroxyl Radical-induced Crosslinking of Thymine and Lysin : Identification of the Primary Structure and Mechanism" Bioorg.Medic.Chem., Letters. 8. 865-870 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyachi, Y.Jokei, K.Oka, M.and Hayashi, T.: "Enzymatic Hydrolysis of Random Copolypeptides Consisting of N-hydroxypropyl-L-asparagine and L-alanine" Eur.Polym.J.35 (3). 395-401 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikada, Y.Nakamura, K.Ogata, S.Makino, K.Tajima, K.Endo, N.Hayashi, T.Fujita, S., Fujisawa, A.Masuda, S.and Oonishi, H.: "Characterization of Ultrahigh Molecular Weight Polyethylene Irradiated with gamma-Rays and Electron Beams to High Doses" J.Polym.Sci., Part A : Polym.Chem.37. 159-168 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jokei, K.Oka, M.Hayashi, T.and Miyachi, Y.: "Enzymatic Hydrolysis of Random Copolypeptides Consisting of N-Hydroxyethyl-L-glutamine and L-alanine, L-leucine, or L-valine" Eur.Polym.J.35 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyachi, Y.Jokei, K.Oka, M.and Hayashi, T.: "Preparation and Properties of Biodegradable Copoly (N-Hydroxyalkyl-D, L-glutamine) Membranes" Eur.Polym.J.35 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miyachi, Y.Jokei, K.Oka, M.and Hayashi, T.: "Biodegradation of Copoly (N-hydroxyalkyl-D, L-glutamine) in vitro" Eur.Polym.J.35 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 古田雅一、大橋 勲、岡 勝仁、林 壽郎: "滅菌を目的とした放射線照射に対するババイン酵素の感受性評価" 生体材料. 17(1). 17-23 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 古田雅一、大橋 勲、岡 勝仁、林 壽郎: "滅菌を目的とした放射線照射による加水分解酵素の特性評価" 食品照射. 33. 41-46 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Nishimoto,S.Morimoto,and S.Fujita: "Hydroxyl radical-induced crosslinking of thymine and lysin : Identification of the primary structure and mechanism" Bioorg.Medic.Chem.Letters. 8. 865-870 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Seo,K.Itoyama,T.Hayashi,et al.: "Spacer effects on enzymatic activity of bromelain immobilized onto porous chitosan beads" Eur.Polym.J.34(7). 917-922 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T,Nishidome,H.Mihara,M.Oka,T.Hayashi,et al.: "Structure and property of model peptides of proline/arginine-rich region in Bactenecin 5" J.Peptide Res.51. 337-345 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] G.Takada,T.Kawaguchi,J.Sumitani,and M.Arai: "Cloning,nucleotide sequencing and transriptional analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase I." J.Ferment.Bioeng.85. 1-9 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] M.Arai,G.Takada,T.Kawaguchi,J.Sumitani: "Biochemical Principles and Mechanism of Biosynthesis and Degradation of Polymers" (ed.A.Steibuechel)Wiley-VCH,Germany, 10 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Hayashi,M.Oka and M.Miyachi: "Molecular Designing of Biodegradable Copolypeptide Membranes" Macromol.Symp.123. 163-175 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Yoda,M.Shimoda,T.Hayashi,et al.: "Morphology Study of Poly(γ-benzyl-L-glutamate)and Polyisoprene Copolymer by Pulsed Proton NMR" Eur.Polym.J.33(6). 815-821 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 米沢司郎、御園生 淳: "個体に見られる放射線適応応答" 原子力学会誌. 39. 532-535 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Takada,T.Kawaguchi,J.Sumitani,and M.Arai: "Cloning,Nucleotide Sequence,and Transcriptional Analysis of Aspergillus aculeatus No.F-50 Cellobiohydrolase 1 (cbhl) Gene" J.Ferment.Bioeng.85. 1-9 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Murai,M.Ueda,M.Arai,et al.: "Genetic Immobilization of Cellulase on the Cell Surface of Saccharomyces cerevisiae" Appl.Microbiol.Biothechnol.48. 499-503 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Seo,K.Itoyama,T.Hayashi et al.: "Spacer Effects on Enzymatic Activity of Bromelain Immobilized onto Porous Chitosan Beads" Eur.Polym.J.34 (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Hayashi,M.Oka,and Y.Miyachi (分担執筆): "Advances in Polymeric Biomaterials Science" CMC Co., 12 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Oka,Y.Hirano,A,Nakajima,and T.Hayashi (分担執筆): "Advances in Polymeric Biomaterials Science" CRC Co., 10 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y. Hirano, K. Madenokouji, A. Nakajima, N. Oka, T. Hayasi: ""Synthesis and Evaluation of Cell-attachment Activity of RGDS-related Molecules"" Trans. Mater. Res. Soc. , Japan. 20. 9-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Kawaguchi, T. Enoki, S. Tsurumaki J. Sumitani, N. Ueda, T. Ooi, M. Arai: ""Cloning and Sequencung of the cDNA encoding β-glucosidase 1 from Aspergikkus aculeatus"" Gene. 173. 287-288 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Oka and T. Hayashi: ""Theoretical Conformational Analysis of Periodic Polypeptides Composed of Repetitive Ala-Pro-Xaa-Yaa Sequence"" Peptide Chem.1995. 469-472 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Yonezawa, J. Misonoh, and Y. Hosokawa: ""Two Types of X-ray-induced Radio-resistance in nice : Presence of 4 dose Ranges with Distinct Biological Effects"" Mutation Res. ,. 358. 237-243 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Fujita, H. Horii, R. Taniguchi, S. Laksmi, and R. Renganathan: ""Pulse Radiolytic Investigations on the Reaction of the 6-yl Radicals of Uracils with Cu (II) -Amino Acid Complexes"" Radiat. Phys. Chem,. 48・5. 643-649 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] G. F. Kutish, Y. Li, M. Furuta, D. L. Rock, and J. L. Van Etten: ""Anlysis of 76kb of the Chlorella Virus PBCV-1 330-kb Genome : Map Position 182 to 258"" Virology. 223. 303-317 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] TOSHIO HAYASHI(分担執筆): "Polymeric Materials Encyclopedia" CRC Press, Inc,7 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Hayashi, M. Oka, and Y. Miyachi(分担執筆): "Biomedical Functions and Biotechnology of Natural and Artificial Polymers" ATL Press, 12 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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