| Project/Area Number |
08558101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo women's Medical University |
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Principal Investigator |
OKANO Teruo Tokyo Women's Medical University, Institute of Biomedical Engineering, Professor, 医学部, 教授 (00130237)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Hideaki Kao Corporation, Research Manager, 主任研究員
YAMATO Masayuki Tokyo Women's Medical University, Institute of Biomedical Engineering, Assistant, 医学部, 助手 (40267117)
KIKUCHI Akihiko Tokyo Women's Medical University, Institute of Biomedical Engineering, Assistant, 医学部, 助手 (40266820)
YOKOYAMA Masayuki Tokyo Women's Medical University, Institute of Biomedical Engineering, Assistant, 医学部, 講師 (20220577)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Poly (N-isopropylacrylamide) / Temperature-responsive / Cell Culture / Culture Dish / Cell Sheet / Recovery / 電子線重合 / 親水性 / 疎水性 / 培養細胞単層シート / 肝実質細胞 / 細胞機能亢進 / 血管内皮細胞 / 細胞活性 |
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| Research Abstract |
Co-culture of multiple cell types, a critical pre-requisite for in vitro tissue construction, often results in domination of co-culture cell populations by one cell type. Natural tissues are often observed to comprise multiple cell types forming distinct layers in stratified architectures. Several cell types are co-organized into defined cell layers in various organs and tissues where stratification of cell layers is critical to cell-cell interaction and, at a larger level, to organ function. We have challenged a novel strategy to reconstruct tissue architectures from cell sheets. A unique temperature-responsive culture surface permits harvest of cultured various cell types as confluent, intact cell monolayer sheets without typical destructive enzymatic treatment. Overlaying two monolayer sheets of hepatocytes and endothelial cells provides a viable construct for in vitro fabricated liver lobule-like tissue. Cultured lamellar cell sheets preserve each cell phenotype and basic cell functions. This general strategy has utility in studies of cell-cell interactions, tissue neogenesis and the design of many types of tissue-engineered biomaterials.
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