| Project/Area Number |
08558103
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
KAJIYA Fumihiko Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions Medical Electronics, Assistant Pro, 医用電子技術科, 講師 (60259596)
GOTO Masami Kawasaki College of Allied Health Professions, Medical Electronics, Associate Pr, 医用電子技術科, 助教授 (50148699)
YADA Toyotaka Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (00210279)
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Associate Professor, 医学部, 助教授 (10152365)
TSUJIOKA Katsuhiko Kawasaki Medical School, Physiolgy, Professor, 医学部, 教授 (30163801)
YAMAMORI Shinji Nihon Kohden Co., R&D Center, Manager
MATSUMOTO Takeshi Kawasaki College of Allied Health Professions Medical Electronics, Assistant Pro (30249560)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1997: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1996: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | high-speed CCD microscopy system / coronary microcirculation / myocardial ischemia / NADH fluorescence / subendocardium / capillary / microheterogeneity / coronary blood flow / 蛍光生体顕微鏡 / 高感度CCD / 血流イメージング / 高速度CCD |
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| Research Abstract |
Goal of this project was to develop a high-speed CCD microscopy system which can be applied to the studies on the evaluation of microvascular behavior and microcirculatory hemodynamics in the subendocardial myocardial layrs which is at greatest risk of ischemia in the heart. We first developed a high speed CCD microscope and evaluated its performance. This system consists of a needle probe, a camera body, a buffer memory and a videocassette recorder. The vascular images focused on the special CCD through a needle-probe are stored on a digital memory every 5 ms (200images/s). A size of visual field at the needle tip is 1.0*0.8 mm^2 and a spatial resolution is about 2mum. The blood flow velocities in the microvessels were visualized by the CCD system using an optically shinning microsphere. This new system successfully visualized flow in myocardial capillary vessels by using microspheres (diameter : 3mum, specific gravity : 1.3) and NADH fluorescence image from the ischemic myocardium. Major findings are as followings : 1) The velocity pattern of microspheres changed dynamically during a cardiac cycle unlike in the capillary vessels of other organs : the velocity waveform was characterized by exclusively diastolic flow with two-peak systolic reverse flow. 2) In response to the decrease in perfusion pressure, subepicardial-and subendocardial arterioles showed reactive dilations. 3) In response to a transient coronary occlusion, ischemic myocardial region as evaluated by the NADH fluorescence showed spatial microheterogeneity and the size of each ischemic region was about several hundred micron meters.
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