| Project/Area Number |
08559016
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
広領域
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
INOUE Tohru National Institute of Health Sciences, Division of cellular and molecular toxicology, director, 安全性生物試験研究センター・毒性部, 部長 (50100110)
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| Co-Investigator(Kenkyū-buntansha) |
SAGA Yumiko National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, section chief, 安全性生物試験研究センター・毒性部, 室長 (50221271)
HIRABAYASHI Yoko National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, senior researrcher, 安全性生物試験研究センター・毒性部, 主任研究官 (30291115)
TAKAGI Atsuya National Instituteof Health Seiences, Divesion of cellular and molecular toxicology, senior researrcher, 安全性生物試験研究センター・毒性部, 主任研究官 (00179417)
藤本 成明 広島大学, 原爆放射能医学研究所・予防腫瘍, 助教授 (40243612)
梅村 隆志 国立医薬品食品衛生研究所, 安全性生物試験研究センター・毒性部, 主任研究官 (50185071)
能美 健彦 国立衛生試験所, 変異遺伝部, 室長 (30150890)
佐井 君江 国立衛生試験所, 毒性部, 研究員 (20195960)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Embryonic stem cell / developmental toxicity / gene mutation / 分化 / 突然変異検出系 |
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| Research Abstract |
The mouse ES cells can be maintained in tissue culture with pluripotency. ES cells can also be induced to differentiate in vitro. These properties make it an excellent tool for the study of early embryogenesis and, potentially, teratogenesis in vitro. We have established genetic reporter systems in the ES cells for the detection of mutations. E.coli xanthine-guanine phosporibosyl transferase (XPRT) gene (gpt) was transfected to the HPRT- ES cells. After transfection with the gpt gene (pSV2-gpt), surviving cells producing XPRT can be selectively grown with xanthine as the sole precursor for guanine nucleotide formation in a medium containing inhibitors (aminopterin and mycophenolic acid) that block de novo purinenucleotide synthesis. After methyl methane sulfonate (MMS) exposure, signifcant increases of 6-TG resistant colony were observed.
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