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Microspectroscopic Studies on Ionic Dye Diffusion in Single Ion-Exchange Beads.

Research Project

Project/Area Number 08640761
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 分離・精製・検出法
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KIM Haeng-Boo  Hokkaido Univ., Graduate School of Sci., Associate Prof., 大学院・理学研究科, 助教授 (40186367)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsion-exchange / particle diffusion / laser trapping / microspectroscopy / confocal microspectroscopy / microparticle / energy transfer
Research Abstract

One of the important factors governing ion-exchange processes is diffusion of an ion in an ion-exchange resin (particle diffusion). Because of a lack of experimental methods, a direct observation of the particle diffusion process is very difficult. Recent progresses in spatially-resolved microspectroscopic techniques such as laser trapping-micro-spectroscopy have provided a powerful means to study various phenomena occurring in microparticles. Here we report direct observation of the particle diffusion process by using laser trapping-microspectroscopy and confocal fluorescence micro-spectroscopy techniques.
Two different methods were applied to determine concentration profiles of ionic dyes in single ion-exchange resins. One was confocal fluorescence microspectroscopy, which provided an information on fluorescence intensity profiles along 3D-axs. The fluorescence intensity profile of Rhodamine B (RhB) along the particle diameter, corresponding to the concentration profile of the dye in … More the resin, was measured. From the change in the profile during RhB diffusion, we determined the diffusion coefficient of RhB in the resin. An energy transfer quenching method was also applied. Initially, RhB was pre-adsorbed homogeneously on the ion-exchange resin. Then, Malachite green (MG) as an energy acceptor was adsorbed on the resin. Since MG diffuses from the resin surface to the inside, the RhB fluorescence is quenched only in a MG-diffused layr. The quenching efficiency corresponds to the volume ratio of the MG-diffused layr to the non-diffused layr in the resin, so that the value gives the penetration depth of MG in the resin. On the basis of the change in the fluorescence intensity of RhB during MG diffusion or that in the penetration depth, we determined directly the diffusion coefficient of MG in the ion-exchange resin.
The single-particles based spectroscopic techniques provide a useful information on the dynamic processes of the ion-exchange and can e further applied to study photochemical processes in single microparticles. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] H.-B., Kim: "Effects of Sample Dimension and Dye Distribution Characteristics in Absorption Microspectroscopy." Analytical Chemistry. 70(1). 51-57 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B., Kim: "Direct Excitation Energy Transfer as aTechnique for in Situ Measurements of Ion-Exchange Processes in Single Polymer Particles." Analytical Chemistry. 70(1). 105-110 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 喜多村 〓: "不均一構造をもつ単一微粒子のレーザー捕捉・顕微分光分析" レーザー研究. 25(11). 754-759 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N., Kitamura: "Effects of Light-Scattering Coefficient of Adsorbent in Diffuse Reflectance Spectroscopy of Dye-Doped Microparticles." Analytical Science. 13(5). 791-796 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N., Kitamura: "Photometric Analyses of Optically-Trapped Single Microparticles in Solution." Analytical Science. 12(1). 49-54 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B., Kim: "In Situ Measurements of Ion-Exchange Processes in Single Polymer Particles:Laser Trapping Microspectroscopy and Confocal Fluorescence Microspectroscopy." Analytical Chemistry. 63(3). 409-414 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B.Kim: "Effects of Sample Dimension and Dye Distribution Characteristics in Absorption Microspectroscopy." Aalytical Chemistry. 70(1). 51-57 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B.Kim: "Direct Excitation Energy Transfer as a Technique for in Situ Measurements of Ion-Exchange Processes in Single Polymer Particles." Aalytical Chemistry. 70(1). 105-110 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N,Kitamura: "Microspectroscopic Analysis of Inhomogeneous Structures in Single Microparticles." The Review of Laser Engineering. 25(11). 754-759 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Kitamura: "Effects of Light-Scattering Coefficient of Adsorbent in Diffuse Reflectance Spectroscopy of Dye-Doped Microparicles." Aalytical Science. 13(5). 791-796 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Kitamura: "Photometric Analyzes of Optically-Trapped Single Microparticles in Solution." Aalytical Science. 12(1). 49-54 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B.Kim: "In Situ Measurements of Ion-Exchange Processes in Single Polymer Particles : Laser Trapping Microspectroscopy and Confocal Fluorescence Microspectroscopy." Aalytical Chemistry. 68(3). 409-414 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.-B.Kim: "Effects of Sample Dimension and Dye Distribution Characteristics in Absorption Microspectroscopy." Analytical Chemistry. 70(1). 51-57 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.-B.Kim: "Direct Excitation Energy Transfer as a Technique for in Situ Measurements of Ion-Exchange Processes in Single Polymer Particles." Analytical Chemistry. 70(1). 105-110 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 喜多村,〓: "不均一構造をもつ単一微粒子のレーザー捕捉・顕微分光分析" レーザー研究. 25(11). 754-759 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Kitamura: "Effect of Light-Scattering Coefficient of Adsorbent in Diffuse Reflectance Spectroscopy of Dye-Doped Microparticles." Analytical Science. 13(5). 791-796 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Kitamura: "Photometric Analyses of Optically-Trapped Single Microparticles in Solution." Analytical Science. 12(1). 49-54 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.-B.Kim: "In Situ Measurements of Ion-Exchange Processes in Single Polymer Particles:Laser Trapping Microspectroscopy and Confocal Fluorescence Microspectroscopy." Analytical Chemistry. 68(3). 409-414 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.-B.Kim: "In Situ Measurements of Ion-Exchange Processes in Single Polymer Particles : Laser Trapping-Microspectroscopy and Confocal Fluorescence Microspectroscopy" Analytical Chemistry. 68(3). 409-414 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Kitamura: "Photometric Analyses of Optically-Trapped Single Microparticles in Solution." Analytical Sciences. 12(1). 49-54 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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