| Project/Area Number |
08640814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生態
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| Research Institution | Chikushi Jogakuen Junior College |
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Principal Investigator |
SASAKI Hiroshi Chikushi Jogakuen Junior College, Department of Pre-school education, Associate professor, 幼児教育科, 助教授 (80270081)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Mustela sibirica / Mustela itatsi / interspecific competition / mitochondrial DNA / cytochrome b / cyt b / mtDNA / cytb |
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| Research Abstract |
The study were conducted from Sep.to Oct.in 1996, from Jan.to Mar.in 1997 and from Jan.to Mar.in 1998 at the 7 places in Fukuoka Pref., 2 places in Saga Pref.and the top area of Mt.Kusenbu at the boundary between Fukuoka and Saga Pref. All study areas were situated in the western part of Seburi Mountains. Weasels were trapped in 4 or 5 nights by 20 wooden box traps. Trap density was one per 50m. Captured weasels were anesthetized, sexed, weighed, and measured. Small pieces of tissues from ears were collected for DNA analysis and transponders were implanted between shoulder blades under skins for identification of individuals. Captured weasels were released at captured site after their recovery.
The cytochrome b genes (402 bases) in the mitochondrial DNA were sequenced for weasels by Mr.Tetsuji HOSODA (Gobo Shoko High School) and Dr.Hitoshi SUZUKI (Environmental Earth Science, Hokkaido Univ.). Eight male and 3 female Siberian weasels (Mustela sibirica) and 5 male Japanese weasels (M.itatsi) were indentified and no difference of the nucleotide sequence was observed among individuals of same species. It was shown indentification of species by morphological characters was valid.
Siberian weasels distributed mainly in lowland and expanded their range to farmlands in mountain areas. Japanese weasels only distributed habitats near upper stream out of farmlands. Both weasels didn't distribute in the natural forest without streams at the top of the mountain and at that with rivers in lowlands. This suggests the population of Japanese weasels has decreased and been isolated in Seburi mountains.
Distribution survey in the other area and telemetric survey will be conducted in the next fiscal year. Nucleus DNA will be analyzed to know the existence of crossbreed between these two mustela and variation in the local populations.
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