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Analysis and modulation of photosynthetic metabolism through the mutagenesis of ferredoxin

Research Project

Project/Area Number 08640828
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionOsaka University

Principal Investigator

HASE Toshiharu  Osaka University, Institute for Protein Research, Professor, たんぱく質研究所, 教授 (00127276)

Co-Investigator(Kenkyū-buntansha) FUJITA Yuichi  Osaka University, Institute for Protein Research, Instructor, たんぱく質研究所, 助手 (80222264)
井手口 隆司  大阪大学, たんぱく質研究所, 助手 (60203121)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsphotosynthetic electron transfer / ferredoxin / glutamate synthase / cyanobacterium / maize / petF / 光独立栄養
Research Abstract

The mechanisms of electron partitioning to several metabolic pathways in photosynthetic cells were studied in focusing structure, function, and gene expression of an electron carrier protein, ferredoxin (Fd). Following results have been obtained in this study.
1) cDNA and gene for a nitrate inducible Fd isoprotein (FdVI) were cloned from maize roots, and its gene expression was found to be regulated with a manner similar to the nitrate assimilatory enzymes.
2) Two different Fd isoproteins, FdI and FdII,were distributed in the mesophyll cells and the bundle sheath cells, respectively, and the cell specific expressions were observed in the transcriptional level. Genes to FdI and FdII were transformed a Fd-deficient cyanobacterium to complement the photosynthetic growth, and the two Fds showed a different activity to C and N assimilations.
3) Study on the functions of recombinant Fds identified amino acid residues involved in the redox properties of the [Fe-2S] cluster and interaction sites to Fd-dependent enzymes. These mutant Fds will be used for modulate the photosynthetic ability of transgenic plants.
4) Genes encoding two glutamate synthase, Fd-GOGAT and NADH-GOGAT,have been cloned, and the gene-disrupted mutants were isolated to study in vivo function of the enzymes. Fd-GOGAT was found to be the isozyme responsible to N assimilation in concert with an elevated CO2 fixation under sufficient light condition.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] I.Taniguchi: "Electrochemical study of biological functions of particular evolutionary conserved amino acidresidues using mutated molecules of maize ferredoxin" Chemistry Letters. 1997. 929-930 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Nakano: "Cloning of maize ferredoxin III Gene : presence of a unique repetitive nucleotide sequence within an intron found in the 5'-untranslated region" Plant Cell Physiology. 38(10). 1167-1170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsumura: "A nitrate-inducible ferredoxin in maize roots" Plant Physiology. 114. 653-660 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Sakakibara: "Molecular identification and characterization of cytosolic isoforms of glutamine synthase in maize root." The Journal of Biological Chemistry. 271. 29561-29568 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Taniguchi: "Electrochemical study of biological functions of particular evolutionary conserved amino acidresidues using mutated molecules of maize ferredoxin" Chemistry Letters. 1997. 929-930 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Nakano: "Cloning of maize ferredoxin III Gene : presence of a unique repetitivenucleotide sequence within an intron found in the 5'-untranslated region" Plant Cell Physiology. 38 (10). 1167-1170 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsumura: "A nitrate-inducible ferredoxin in maize roots" Plant Physiology. 114. 653-660 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Sakakibara: "Molecular identification and characterization of cytosolic isoforms of glutamine synthase in maize root." The Journal of Biological Chemistry. 271. 29561-29568 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Taniguchi: "Electrochemical study of biological functions of particular evolutionary conserved amino acid residues using mutated molecules of maize ferredoxin" Chemistry Letters. 1997. 929-930 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] R.Nakano: "Cloning of maize ferredoxin III Gene : presence of a unique repetitive nucleotide sequence within an intron found in the 5'-untranslated region" Plant Cell Physiology. 38(10). 1167-1170 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Matsumura: "A nitrate-inducible ferredoxin in maize roots" Plant Physiology. 114. 653-660 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Sakakibara: "Molecular identification and characterization of cytosolic isoforms of glutamine synthase in maize root." The Journal of Biological Chemistry. 271. 29561-29568 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Matsui: "Bell pepper fruit fatty acid hydroperoxide lyase is a cytochrome P450 (CYP74B)." FEBS Letter. 394. 21-24 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Fujita: "Identification of the chlB gene and the gene product essential for the light-independent chlorophyll biosynthesis in the cyanobacterium Plectonema boryanum." Plant Cell Physiology. 37. 313-323 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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