• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genetic regulation of dimorphic transition between yeast and hyphal form growth in the imperfect yeast, Candida tropicalis.

Research Project

Project/Area Number 08640846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionNARA WOMEN'S UNIVERSITY

Principal Investigator

SUZUKI Takahito  DEPARTMENT OF BIOLOGY FACULTY OF SCIENCE NARA WOMEN'S UNIVERSITY,FULL PROFESSOR, 理学部, 教授 (60144135)

Co-Investigator(Kenkyū-buntansha) KAMIHARA Teijiro  DEPARTMENT OF LIFE AND SPORT SCIENCE OSAKA UNIVERSITY OF HEALTH AND SPORT SCIENC, 生涯スポーツ学科, 教授 (90025982)
IWAGUCHI Shin-ichi  DEPARTMENT OF BIOLOGY FACULTY OF SCIENCE NARA WOMEN'S UNIVERSITY,ASSISTANT PROFE, 理学部, 講師 (40263420)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsCandida tropicalis / hyphal formation / ethanol / substractive cloning / NMT1 / thiamine / NMTI遺伝子 / チアミン / candida tropicalis / NMT1遺伝子 / チロシンリン酸化酵素 / リボゾームタンパク
Research Abstract

Ethanol caused mycelial growth in the imperfect yeast Candida tropicalis Pk233. Cultivation with ethanol gave biphasic growth curves. During the first growth phase in the ethanol culture, there was an accumulation of swollen spherical yeast cells, instead of the oblong ones observed in the control culture, followed by the appearance of spherical daughter cells in chains. During the second growth phase, hyphal cells appeared, projecting from the swollen yeast cells. Subtractive cloning of genes was performed on the cDNAs of the ethanol culture, from which the cDNAs of the control cultur had been subtracted by hybridization. Isolated genes specific for the process of hyphal formation included nmt1 homolog and URP2 homolog. Correlation was suggested of the process of ethanol-induced hyphal formation with thiamine metabolism, since the mycelial growth was abolished by addition of thiamine.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Suzuki T, Imanishi Y, Iwaguchi S, Kamihara T: "Depolarized growth procedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis." Microbiology. 144. 403-410 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano S, Inoue S, Nouchi R Mogami k, Suzuki T: "Antifungal nickel (II) complexes derived from amino sugars against pathogenic yeast,Candida albicans" Journal of Inorganic Biochemistry. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakaguchi S, Shibuya K, Iida H, Anraku Y, Suzuki T: "Role of Ca^<++> in hyphal and yeast-form growth in Candida albicans" Mycoscience. 38. 215-225 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木 孝仁, 今西 由巳, 岩口 伸一, 上原 悌次郎: "Candida tropicalisの菌糸形成に特異的な遺伝子の検索" 日本医真菌学会誌. 39 (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木 孝仁: "医真菌のゲノムプロジェクト" 化学と生物. 34 (6). 397-398 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 鈴木 孝仁: "細胞の極性成長にはたらく共通な遺伝子像" 化学と生物. 35 (2). 119-120 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sakaguchi, S., Shibuya, K., Iida, H., Anraku, Y.and Suzuki, T.: "Role of Ca^<++> in hyphal and yeast-form growth in Candida albicans : Growth regulation by altered extracellular and intracellular free Ca^<++> concentrations. Mycoscience 38,215-225." Mycoscience. 38. 215-225 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, S., Inoue, S., Nouchi, R., Mogami, K.and Suzuki, T.: "Antifungal nickel(II) complexes derived from amino sugars against pathogenic yeast, Candida albicans." Journal of Inorganic Biochemistry. (accepted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, T., Imanishi, Y., Iwaguchi, S.& Kamihara, T.: "Depolarized growth precedes filamentatin during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis." Microbiology. 144. 403-410 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki,T,Imamishi,Y,Iwaguchi,S-I,Kamihara,T.: "Depolarized growth procedes filamentation during the process of ethanol-induced pseudohyphal formation in the yeast Candida tropicalis." Microbiology. 144・(2). 403-410 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yano,S,Inoue,S,Nouchi,R,Mogami K,Suzuki T: "Antifungal nickel (II) complexes derived from amino sugars against pathogenic yeast,candida albicans." Journal of Inorganic Biochemistry. 印刷中 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakaguchi S,Shibuya K,Iida H,Anraku Y,Suzuki T: "Role of Ca^<++> in hyphal and yeast-form growth in Candida albicans." Mycoscience. 38・(2). 215-225 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木孝仁・今西由巳・岩口伸一・上原悌次郎: "Candida tropicalisの菌糸形成に特異的な遺伝子の検索" 日本医真菌学会誌. 39・(3). 発表予定 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木 孝仁: "医真菌のゲノムプロジェクト" 化学と生物. 34(6). 397-398 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鈴木 孝仁: "細胞の極性成長にはたらく共通な遺伝子像" 化学と生物. 35・(2). 119-120 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 坂口修一,渋谷恭子,飯田秀利,安楽泰宏,鈴木孝仁: "Roles of Ca^<2+> in hyphal and yeast-form growths in Candida albicans Growth regulation by altered extracellular and intracellular tree Ca^<2+> conantrations." Myco science. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木孝仁: "医真菌のゲノムプロジェクト" 化学と生物. 34巻・6号. 397-398 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木孝仁: "細胞の極性成長にはたらく共通な遺伝子像" 化学と生物. 35巻・2号. 119-120 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上原悌次郎・今西由己,岩口伸一・鈴木孝仁: "Stress response leading to pseudomycelial development in the Yeast Candida tropicalis." Proceeding of International Conference:Microbial response to stress.(1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi