| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Hydra species are members of the phylum Cnidaria, and are equipped with special kinds of cells (nematocytes) which are used for the capture of prey, defense and attaehmcnt. Because of such interesting multi-functions and the compticaled structue of their extrusome (nematocysis), nematocytes have held the interest of many investigators. There has been, hower, no good method to demonstrate visually the morph of each type of nematocyst during their formation, and therefor emost observations on the morphogenesis of nematocysts have been made without identification of the nematocyst type.
We previously produced AE03 as a monoclonal antibody which recognized mucous granules in the basal disk gland cells and the secreted mucus with which hydrae stick on substratc. AE03 also recognized atrichous isorhiza, one of the four types of nematocyst present in tentaelcs, and the nematoblasts of atrichous isorhizas present in the body column.
with this monoclonal antibody AE03, we could, for the first time, observe the detailed morphogenesis of one specific nematocyst, atrichous isorhiza, from the beginning of its morphogenesis. The elongation and invagination processes of external tubes were confirmed and it was also suggested that the external tubes corresponded to the thread of discharged nematocysts. We also obtained some interesting suggestion on the synchrony of the nematocyst morphogenesis in one nest (the synchrony depends on the cytoplasumic bridges between the nematoblasts) and the orientation of nematocyte during the migration toward the tentacles (the nematocytes direct the top of their nematocysts down toward the foot).
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