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Molecular mechanisms of androgen receptor expression

Research Project

Project/Area Number 08640850
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 生物形態・構造
Research InstitutionJuntendo University School of Medicine

Principal Investigator

MATSUMOTO Akira  Juntendo Univ.Sch.Med., Assoc.Prof., 医学部, 助教授 (80053263)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsrat / lumbar spinal cord / motoneuron / androgen / androgen receptor / aging / immunohistochemistry
Research Abstract

Motoneurons of the spinal nucleus of the bulbocavernosus (SNB) of male rats innervate the perineal striated muscles bulbocavernosus and levator ani that attach to the penis. They have an important role in copulatory behavior, which is sensitive to alterations in circulating levels of androgen. Regulation of androgen receptor expression seems to be one of the important processes for functions of the androgen receptor (AR)-containing neurons under the influence of androgen. To clarify regulatory mechanisms of AR expression, we examined androgenic regulation of AR immunoreactivity in the SNB motoneurons in adult male rats by immunohistochemistry using the polyclonal antibody, PG21. In intact controls, intense AR immunoreactivity was confined to the cell nucleus. Androgen withdrawal significantly reduced both the intensity of AR immunoreactivity in the nuclei and number of AR immunoreactive nuclei of the SNB motoneurons within 1 day of castration. AR immunostaining in the nucleus was completely eliminated 5 days following castration. These changes were prevented by replacement of testosterone propionate (TP). The number of AR immunoreactive nuclei recovered to about half of the control levels within 20 min or 1 hr of TP administration to the castrated males, although the intensity of AR immunoreactivity was very weak. Both the intensity of AR immunoreactivity and number of AR immunoreactive nuclei recovered to the control levels 2 or 6 hr after TP injection. In addition, AR immunoreactivity of SNB motoneurons was examined in young and old male rats. In old animals, both the intensity of AR immunoreactivity in the nuclei and number of AR immunoreactive nuclei of the SNB motoneurons were significantly reduced. Plasma levels of testosterone in old animals were significantly smaller than those in young ones. These results suggest that androgen causes a significant up-regulation in AR expression of SNB motoneurons.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Matsumoto A et al.: "Androgenic regulation of androgen receptor immunoreactive in motoneurons of the spinal nucleus of the bulbocavernosus of male rats." J Neuroendocrinol. 8. 553-559 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A et al.: "Immunohistochemical analysis of androgen receptor in the abdominal glands of the cloaca of male red-belied newts, Cynops pyrrhogaster." Zool Sci. 13. 429-433 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松本 明: "神経回路の性分化と性ステロイドに対する可塑性" BRAIN MEDICAL. 8. 243-248 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A: "Hormonally induced neuronal plasticity in the adult motoneurons." Brain Res Bull. 44. 539-547 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A et al.: "Hormonal alteration of synaptic input to Mauthner cells of the adult male red-bellied newts." Zool Sci. 14. 651-657 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A: "Androgen regulates expression of androgen receotors in the spinal motoneurons." Ann New York Acad Sci. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A,Arai Y,Prins GS: "Androgenic regulation of androgen receptor immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus of male rats." J Neuroendocrinol. 8. 553-559 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A,Arai Y,Toyoda F,Kikuyama S,Prins GS: "Immunohistochemical analysis of androgen receptor in the abdominal glands of the cloaca of male red-bellied newts, Cynops pyrrhogaster." Zool Sci. 13. 429-433 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A: "Hormonally induced neuronal plasticity in the adult motoneurons." Brain Res Bull. 44. 539-547 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A,Toyoda F,Kouki T,Kikuyama S: "Hormonal alteration of synaptic input to Mauthner cells of the adult male red-bellied newts." Zool Sci. 14. 651-657 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A: "Androgen regulates expression of androgen receptors in the spinal motoneurons." Ann New York Acad Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A,Arai Y: "Sexual differentition of neuronal circuitry in the neuroendocrine hypothalamus." Biomed Rev. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A,Prins GS: "Age-dependent changes in androgen receptor immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus of male rats." Neurosci Lett. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumoto A: "Hormonally induced neuronal plasticity in the adult motoneurons." Brain Res Bull. 44. 539-547 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto A et al.: "Hormonal alteration of synaptic input to Mauthner cells of the adult male red-bellied newts." Zool Sci. 14. 651-657 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto A: "Audrogen regulates expression of androgen receptors in the spinal motoneurons." Ann New York Acad Sci. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto A et al.: "Sexual differentiation of neuronal circuitry in the neuroendocrine hypothalamus." Biomed Rev. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto A et al.: "Age-dependent changes in androgen receptor immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus of male rats." Neurosci Lett. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 松本明: "性ホルモンによる組織構築の性差" 神経研究の進歩. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto A et al.: "Immunohistochemical analysis of androgen receptor in the abdominal glands of the cloaca of male red-bellied newts,Cynopus pyrrhogaster." Zoological Science. 13. 429-433 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsumoto A et al.: "Androgenic regulation of androgen receptor immunoreactivity in motoneurons of the spinal nucleus of the bulbocavernosus." J Neuroendocrinology. 8. 533-539 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsumoto A.: "Hormonally induced neuronal plasticity in the adult motoneurons." Brain Research Bulletin. (in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsumoto A.: "Androgen regulates expression of androgen receptors in the spinal motoneurons." Annals of the New York Academy of Sciences. (in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松本 明: "神経回路網の性分化と性ステロイドに対する可塑性" BRAIN MEDICAL. 8. 243-248 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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