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Identification of Drinking Center and Its regulatory substances isolated from the Eel Gut

Research Project

Project/Area Number 08640864
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

ANDO Masaaki  Hiroshima University, Faculty of Integrated Arts and Sciences, Professor, 総合科学部, 教授 (10100976)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsRegulation of drinking behavior / water absorption / brain / drinking center / dorsal part of the medulla oblongata / basal part of the saccus vasculosus / eels / 血液嚢 / 飲水行動 / アンギオテンシン / アンギオテンシン受容体 / 飲水促進因子 / 飲水抑制因子 / 血液・脳関門 / 脳地図
Research Abstract

To compensate for water liss in sea water, eels drink the sea water and absorbed it through the intestine. However, the intestine can not absorb water from the sea water directly. If highly concentrated NaCl enters into the intestine, eels stopped the drinking, which was due to luminal Cl^- Na^+ itself enhanced the drinking behavior. The drinking behavior was altered 20 min after Na^+ or Cl^- reached to the intestine. Therefore, we expected some hormonal substances secreted from the intestine might regulate drinking behavior. When angiotensin II (ANGII), histamine (HA), serotomin (5-HT), carbochol, isoproterenol, prolactin, or substance P was injected into the posterior cardinal vein, the drinking was depressed. On the other hand, atrial natriuretic peptide (ANP), vasotocin, smatostatin, phenylephrine, vasoactive intestinal peptide (VIP), cholecystokinin (CCK8), peptide YY,and eel intestinal pentapeptide (EIPP) stopped the drinking. Because all these reagents act from the blood, the dr … More inking center was suspected to be a part of the circumventricular organs, where no blood-brain barrier exist. When Evans Blue was injected intraperitoneally, dorsal part of the medulla oblongata, ventral part of the cerebelum and basal part of the saccus vasculosus in the brain were atained, indicating absence of blood-brain barrier in these areas. We also observed that the effects of HA and 5-HT were via ANGII,suggesting that ANGII is one of candidates which act directly on the brain. Therefore, in the next experiments, ANGI-fluorescen was applied to the section of the eel brain. Specific fluorescence was observed at the dorsal part of the medulla oblongata and at the basal part of the saccus vasculosus. Altogether, the dorsal part of the medulla oblongata and the basal part of the saccus vasculosus may be a part of drinking center in eels. Although EIPP isolated from eel intestine depressed drinking behavior, EIPP was not detected in the incubation medium by HPLC after perfusing the intestine with high concentrated C^-. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ando, M & Nagasima, K: "Intestinal Na^+and Cl^- levels control drinking behavior in the seawater adapted eel Anguilla Japonica" J.Exp.Biol.199. 711-716 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uesaka, T.et al.: "Effects of eel neuropeptide Y on ion transport across the seawater eel intestine" Zool.Sci. 13. 341-346 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, M et al.: "Regulation of atrial contraction in the seawater-adaptedeel,Anguilla japonica" Comp.Biochem.Physiol.113A. 165-172 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uesaka, T.: "Synergistic action of neuropeptide Y and adrenaline in the eel atrium" J.EXP.Biol.199. 1873-1880 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokota, S.et al.: "Ion transport across the skin of the mudskipper Periophthalmus modestus" Comp.Biochem.Physiol.118A. 903-910 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kim, H.T.et al.: "Novel [3h]clonidine dinding sites in the intestine of the eel acclimated to sea water" Zool.Sci.15(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando, M., Nagashima, K.: "Intestinal Na^+ and Cl^- levels control drinking behavior in the seawater-adapted eel Anguilla japonica." J.Exp.Biol.199. 711-716 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uesaka, T., Yano, K., Sugimoto, S., Ando, M.: "Effects of eel neuropeptide Y on ion transport across the seawater eel intestine." Zool.Sci.13. 341-346 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuda, M., Uesaka, T., Furukawa, Y., Ando, M.: "Regulation of atrial contraction in the seawater-adapted eel, Anguilla japonica." Comp.Biochem.Physiol.113A. 165-172 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uesaka, T.: "Synergistic action of neuropeptide Y and adrenaline in the eel atrium." J.Exp.Biol.199. 1873-1880 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yokota, S., Iwata, K., Fujii, Y., Ando, M.: "Ion transport across the skin of the mudskipper Periophthalmus modestus." Comp.Biochem.Physiol.118A. 903-910 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kim, H.T., Sakamoto, T., Ando, M.: "Novel [^3H] clondine binding sites in the intestine of the eel acclimated to sea water." Zool.Sci.15 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ando, M & Nagashima K.: "Intestinal Na^+ and Cl^- levels control drinking behavior in the seawater adapted eel anguilla japonica" J.Exp.Biol.199. 711-716 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uesaka T.et al.: "Effecto of eel neuropeptide Y on ion transport across the seawater eel intestine" Zool.Sci.13. 341-346 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuda, M.et al.: "Regulation of atrial contraction in the seawater-adapted eel, anguilla japonica" Comp.Biochem. Physiol.113A. 165-172 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uesaka, T.: "Synergistic action of neuropeptide Y and adrenaline in the eel atrium" J.Exp.Biol.199. 1873-1880 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yokota, S.et al.: "Ion transport across the skin of the mudskipper, Periophthalmus modestus" Comp.Biochem. Physiol.118A. 903-910 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kim, H.T.et al.: "Novel 〔^3H〕 clonidine binding sites in the intestine of the eel acclimated to sea water" Zool.Sci.15 (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] M. Ando and K. Nagashima: "Intestinal Na^+ and Cl^- control the drinking behavior in the seawater-adapted eel Anguilla japonica" J. Exp. Biol.199. 711-716 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Uesaka et al.: "Effects of eel neuropeptide Y on ion transport across the seawater eel intestine" Zool. Sci.13. 341-346 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Uesaka: "Synergistic action of neuropeptide Y and adrenaline in the eel atrium" J. Exp. Biol.199. 1873-1880 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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