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The pattern of geographic variation in karyotypes and mitochondrial DNA in chromosomal hybrid zones of Nelima nigricoxa (Arachnida, Opiliones)

Research Project

Project/Area Number 08640888
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionTottori University

Principal Investigator

TSURUSAKI Nobuo  Tottori University, Faculty of Education, Associate Professor, 教育学部, 助教授 (00183872)

Co-Investigator(Kenkyū-buntansha) UESHIMA Rei  The University of Tokyo, Graduate School of Sciences, Lecturer., 大学院・理学研究科, 講師 (20241771)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsChromosomes / Hybrid zone / Speciation / MtDNA / Nelima nigricoxa / Opiliones / Arachnida / 地理的変異 / 核型分化
Research Abstract

To reveal the nature and formation process of chromosomal hybbrid zones in Nelima nigricoxa, we analyzed karyotypes, allozymes, and mtDNA for various populations of the species and the closely related congeners. A total of seven karyotypes were recongnized in Nelima nigricoxa : 2n=20 (Kyushu, Shikoku, and Chugoku district westward of the Asahi River), 2n=18a (West of Mt.Daisen, Tottori Pref.and Kinki district), 2n=18b (north of Okayama city), 2n=16 (Chugoku mountains eastward from the Asahi River), 2n=22a (Izu-Hakone), 2n=22b (Miura Peninsula), 2n=22c (Mt.Kiyosumi, Chiba Pref.). Of these, each of the three adjoining karyotype pairs (2n=20/18a, 2n=18a/16, and2n=16/18a) forms a hybrid zone with a width less than 1-3 km. In a population (Shimimu-dani) in a hybrid zone between 2n=16 and 18a in Sekigane-cho, Tottori Prefecture, the ratio of individuals with 2n=17 chromosomes was significantly lower than the expected ratio from the Hardy-Weinberg theorem (<0.02). The relative fitness of indi … More viduals with 2n=17 was 0.4 when taking 2n=16 equals 1. The figure was much lower than the relative fitness of heterozygotes, 0.7 obtained in a population (Kawadoko) in a hybrid zone (2n=18/20) in Mt.Daisen. Difference in the size of chromosomes that pertain to the chromosome rearrangement may explain this result.
We analyzed sequences of about 1 kilobase pairs of CO I gene coded by the mtDNA for several population of N.nigiricoxa and some populations of the closest relatives. The phylogeny obtained was : (nigricoxa Mt.Kiyosumi, Chiba, 2n=22, genufusca Sapporo, 2n=22) (genufusca Tokusen Falls, Yamaguchi, 2n=22 (nigricoxa Tokusen Falls, Yamaguchi, 2n=20 (nigricoxa Mt.Senjo, 2n=18 (nigricoxa Makiyaha Mt.Daisen 2n=20, nigricoxa 2n=20 Makihara Mt.Daisen)))) and the bootstrap probability for each branch was 100% without exception. It was confirmed that grographic differentiation can be detected also in mtDNA level among some morphologically non-differentiatied populations of Nelima nigricoxa in the Chogoku district. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Tsurusaki, N.& Shimada, T.: "Seasonal and geographic variation of the number of B-chromosomes in Metagagrella tenuipes (Arachnida : Opiliones)" Zoological Science. 13 (Suppl).39-39 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shear, W.A., Tanabe, T.& Tsurusaki, N.: "Japanese chordeumatid millipeds.IV.The new genus Japanoparvus (Diplopoda,Chordeumatida,Hoffmaneumatidae)" Myriapodologica. 4 (11). 89-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsurusaki, N., Fujioka, M.& Aoki, N.: "Geographic differentiation of Karyotypes in the Nelima genufusca group (Arachnida,Opiliones)" Zoological Science. 14 (Suppl.) (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsurusaki, N.and Shimada.T.: "Seasonal and geographic variation of the number of B-chromosomes in Metagarella tenuipes (Arachnida : Opiliones)." Zoological Science. 13 (Suppl.) (Abstruct). 39 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shear, W.A.., Tanabe, T.And Tsurusaki, N.: "Japanese chordeumatid millipeds. IV.The new genus Japanoparvus (Diplopoda, Chordeumatida, Hoffmaneumatidae)" Myriapodologica. 4 (11). 89-99 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsurusaki, N., Fujioka, M., and Aoki, N.: "Geographic differentiation of karyotypes in the Nelimaa genufusca group (Arachnida, Opiliones)" Zoological Science. 14 (Suppl.) : (Abstract) (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsurusaki,N.& Shimada,T.: "Seasonal and geographic variation of the number of B-chromosomes in Metagagrella tenuipes (Arachnida : Opiliones)" Zoological Science. 13 (Suppl.). 39-39 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shear,W.A., Tanabe,T.& Tsurusaki,N.: "Japanese chordeumatid millipeds.IV. The new genus Japanoparvus (Diplopoda,Chordeumatida,Hoffmaneumatidae)" Myriapodologica. 4(11). 89-99 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsurusaki,N., Fujioka,M.& Aoki,N.: "Geographic differentiation of Karyotypes in the Nelima genufusca group (Arachnida,Opiliones)" Zoological Science. 14 (Suppl.) (in press). (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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