| Project/Area Number |
08650490
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
計測・制御工学
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| Research Institution | Osaka University |
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Principal Investigator |
NAKAMURA Osamu School of Engineering, Osaka University, Associate Professor, 工学部, 助教授 (90192674)
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| Co-Investigator(Kenkyū-buntansha) |
KAWATA Yoshimasa Shizuoka University, Faculty Engineering, Associate Professor, 工学部, 助教授 (70221900)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | two photon fluorescence / confocal microscopy / phase conjugation / レーザー顕微鏡 / 表面プラズモン |
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| Research Abstract |
We investigated fluorescence microscopy for measuring the faint fluorescent light from organic molecules such as DNA.We developed a two-photon fluorescence microscope using a near-infrared pulsed laser, and 4pi confocal and double-pass confocal microscopes with a self-conjugated mirror. We theoretically analyzed three developed microscopes and succeeded in capturing the images.
1) Two-photon fluorescence microscope
We developed a two-photon fluorescence microscope with a near-infrared pulse laser (Ti : Sapphire laser : 750nm). By use of developed system, we observed an eye of mouse embryo stained with DAPI.We succeeded in obtaining optical sectioning images by scanning the focal plane without slicing the sample. The system provides the images at 250 mum depth in the sample. We confirmed that the microscope had high resolution through measurement of a stained nucleus.
2) 4pi confocal microscope with a phase-conjugate mirror
We developed a 4pi confocal microscope with a phase-conjugate mirror. As the phase-conjugate mirror, we used a Ce doped BaTiO_3 crystal. By using of the microscope, we measured an axial response of the system. The result shows that a center peak has -0.25 mum width, which agrees with theoretical value (lambda/2). However, low reflectivity (-60%) of the mirror causes low visibility of interference in the sample.
3) Double-pass confocal microscope with a phase-conjugate mirror
We observed a diatom by using the developed double-pass confocal microscope with a phase-conjugate mirror. The resolution of the images was good even after passing through the sample twice. Due to the phase-conjugate property, the image given with the developed microscope was aberration free.
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