| Project/Area Number |
08650953
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Hiroshima-Denki Institute of Technology |
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Principal Investigator |
SASAKI Ken Hiroshima-Denki Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (80140565)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAOKA Takashi Hiroshima-Denki Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (60156388)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | self-flocculation / photosynthetic bacteria / biodegradable plastic / sediment mud / PHB / Rhodovulum sp. / Rhodobacter |
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| Research Abstract |
Focusing on the purification of sediment mud and bioconversion of mud to biodegradable plastic, firstly, chemical analyses of sediment mud were carried out. Sediment mud was consisted of 5% organic matters and 95% of sea sund and contained large amount of phosphorous compounds, After anaerobic digestion of mud suspension (200 g/l artificial sea wate) at 30 C for 7 days, ca. 2 g/l of acetic acid and Ca. 20 mg/l of phosphate ion was released into the broth. Using this anaerobic digestion liquor, a photosynthetic bacterial cultivation was carried out under anaerobic light condition (10 klux illumination) at 30 C.After 3 days culture, ca. 3 g/l of biomass was produced and almost phosphate was removed. Biomass produced contained 62% (dry basis) of polyhydroxybutyrate (PHB). Thus, the possibility of simultaneous purification of sediment mud and production of PHB was observed.
Furthermore, high cell density culture of a newly isolated photosynthetic bacteria, Rhodovulum sp. which had the sel-flocculating ability was carried out using a new single tower bioreactor system to purify the anaerobic digestion liquor under aerobicculture condition eliminating light illumination. By this culture system, maximum biomass concentration attained 42.5 g/l after 750 hour culture with artifitial digestion liquor (acetic acid was a carbon source) and PHB content was ca. 30% even under aerobiccondition. In addition, a computer simulation was carried out using several biochemical engineering constants such as growth yield and some specific consumption rates. As the results, the estimations were in good agreement with the data obtained by experiments.
Thus, a new bioreactor system become possible to develop by these investigations using this self-immobilizing photosynthetic bacteria. This indicates the possibility of the purification of sediment mud and simultaneous production of biodegradable plastic from sediment mud.
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