| Project/Area Number |
08650954
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
生物・生体工学
|
|---|
| Research Institution | Kumamoto Institute of Technology |
|---|
Principal Investigator |
OGAWA Takahira Kumamoto Institute of Technology, Faculty of Engineering, Professor, 工学部, 教授 (40029244)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUOKA Masayoshi Kumamoto Institute of Technology, Faculty of Engineering, Associate Professor, 工学部, 助教授 (10121667)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
|
|---|
| Keywords | Ethylene / Cyanobacteria / Ethylene-forming Enzyme / Synechococcus sp. / Phosphoenolpyruvate carboxylase / Carbonic anhydrase / PEPC / 二酸化炭素 / 代謝工学的研究 / psbAI |
|---|
| Research Abstract |
Photosynthetic conversion of carbon dioxide to ethylene was studied using the recombinant cyanobacterium, Synechococcus sp.stain PCC 7942 IEK 2-2 which had been integrated the ethylene-forming enzyme (EFE) gene from Pseudomonas syringae pv.phaseolicola PK2 into the ORF of psbAI.Since the EFE reaction catalyzes the conversion of 2-oxoglutarate to 1 mole of ethylene and 3 moles of carbon dioxide, the metabolite shortage in the tricarboxylic acid cycle of the recombinant cyanobacterium will be resulted. In this reason, carbon dioxide recycling system such as phoshoenolpyruvate carboxylase (PEPC) and carbonic anhydrase (CA) genes were introduced into the recombinant cyanobacterium IEK2-2. The plasmids named pEXE3DELTA8, pEXE3DELTA8/PEPC1 and pEXE3DELTA8/PEPC1/CA1 were constructed on the shuttle vector pUC303 and transformed IEK2-2. The increase of ethylene production was observed in the recombinant cyanobacterium harboring IEK2-2/pEXE3DELTA8/PEPC1. The amount of carbon incororated into ethylene was calculated as a percentage of the total carbon fixed, and the maxiumum value of which was 13.0% and 11.6% in the recombinant cyanobacteria harboring IEK2-2/pEXE3DELTA8/PEPC1 and IEK2-2/pEXE3DELTA8/PEPC1/CA1, respectively.
|
