Fluormetric Study on interaction of Media Having Internal Hadrophobic Environment with Analytical Reagents
| Project/Area Number |
08650961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業分析化学
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| Research Institution | Shizuoka University |
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Principal Investigator |
YAMADA Shinkichi Shizuoka University, Faculty of Engin., prof., 工学部, 教授 (60022737)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Motoshi Shizuoka university, Faculty of Engin., Assoc.prof., 工学部, 助教授 (80022242)
ITOH Takushi Shizuoka University, Faculty of Engin., prof., 工学部, 教授 (20022022)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Cyclodextrin / Surfactant / Fluorometric reagent / Inclusion reaction |
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| Research Abstract |
Under the projects proposed some experiments were carried out and the following results were obtained.
1. Inclusion Equilibria of flavones and coumarins with beta-cyclodextrin(CD) have been studied fluorometrically in aqueous organic solvents. Dependence of the apparent inclusion constants on the concentration of organic solvent could be analyzed by taking the substitution of organic solvent included in beta-CD with these fluorometric regents into consideration. The constants thus estimated for the inclusion of organic solvent with beta-CD were found to be correlated with solubility parameter of respective organic solvents.
2. Capacity factor for flavones, chromons and coumarins on RPLC column has been analyzed as a function of the concentration of beta-CD in mobile phase. The retention of these reagents on the column could be quantitatively interpreted by taking the inclusion of the reagents with beta-CD in mobile phase into consideration. Effect of structural characteristics of the reagents on their partiton between stationary phase and mobile phase was discussed.
3. Interraction of flavones with cationic and neutral surfactant micelles has been analyzed as a function of the concentration of respective micelles. Association constant of the reagents with the micelles could be interpreted by taking the electrostatic and the hydrophobic interactions between the reactants into consideration. By comparing the present results with those obtained in beta-CD system, factors affecting the association eqilibria and the resulting fluorescence enhancement were discussed.
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Report
(3 results)
Research Products
(16 results)
