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Molecular analysis of ultraviolet (UV)-B signal transduction and a mechanism of UV-tolerance in agronomical plants.

Research Project

Project/Area Number 08660001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHirosaki University

Principal Investigator

AKADA Shinji  Hirosaki University, Gene Research Center., Associate professor, 遺伝子実験施設, 助教授 (10250630)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsFlavonoids / Soybean / Chalcone synthase genes / Ultraviolet (UV) -B / MYB-homologous / Rhisobium-legume symbiosis / 紫外線誘導
Research Abstract

Flavonoids are belived to play an important role in protection against biotic and abiotic attacks. The primary aim of this project is to elucidate the molecular mechanism of flavenoid biosynthesis in response to the emviromnental stimuli.
Chalcone synthase (CHS), the first step enzyme of flabonoid biosynthesis, is encoded by seven genes in soybean. In this research, we quantitatively analyzed the expression levels of each member in soybean seedlings. Expression of chs7 was induced very early reaching its peak within 6 hours post imbibition. Ultraviolet (UV) -B response of the gene family was found to emerge during 24 hours of imbibition, suggesting a developmental control of the machinery in the germinating embryo. In three-day-dark-grown seedlings, all the seven genes responded to white light and showed up-regulation by UV-Bwith substantial variation in their response among the members.
Some flavonoids are also reported to function as inducer of Rhizobium-legume symbiosis and this is inhibited by nitrate in the growth medium. Here we investigated if expression of CHS genes in soybesn is regulated by the presence of nitrate in the medium and found that expression of gene 7 was inhibited by nitrate and that this kind of response to the nitrogen compound was specifically observed in legume plants.
Most of the light-responsive chs genes contain an H-box except for the least responsive gene, chs2. The H-box is reported to be recognized by MYB-homologous P element in maize, which activates in trans flavonoid genes. In this project, we isolated the whole region of the soybean NYB-homologous genes, Gmmyb10 and Gmmyb29, whose expression was found up-regulated by UV-B.We intend to use thses genes to manipulate flavonoid biosynthesis in legume.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Kaimori, N.: "Asymmetric somatic cell hybrids between alfalfa and birdsfoot trefoil." Breeding Science. 48. 29-34 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, T.: "Genomic nucleotide sequence of a ripening related l-aminocyclopropane-l-carboxylate syntase gene(MdACS-1)in apple." Plant Physiology. 113. 1465-1465 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishikawa, R.: "Complex constitutive nature of Japanese upland rice,Oryza sativa L." Eupytica. 98. 197-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 赤田 辰治: "紫外線UV-Bによるダイズカルコンシンターゼ遺伝子の発現調節と転写因子の探索" 東北大学遺伝研究センター通信. 33. 1-3 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kaimori, N., M.Senda, R.Ishikawa, S.Akada, T.Harada and M.Niizeki: "Asymmetric somatic cell hybrids between alfalfa and birdsfoot trefoil." Breeding Science. 48. 29-34 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, T., T.Sunako, W.Sakuraba, S.Goto, M.Senda, S.Akada, R.Ishikawa and M.Niizeke: "Genomic nucleotide sequence of a ripening related 1-aminocyclopropane-1-carboxylate syntase gene (MdACS-1) in apple." Plant Physiol.113. 1465-1465 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishikawa, R., Y.Harata, Y.Tuchimine, M.Senda, S.Akada, T.Harada and M.Niizeki: "Complex constitutive nature of Japanese upland rice, Oryza sativa L." Eupytica. 98. 197-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akada, S: "Ultraviolet-B regulation of soybean gene chalcone synthase gene expression and a molecular search of the transcription factors." Tohoku University IGE briefings. 33. 1-3 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada,T.: "Genomic nucleotide sequence of a ripening related 1-aminocyclopropane-1-carboxylate syntase gene(MdACS-1)in apple." Plant Physiology. 113. 1465-1465 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishikawa,R.: "Complex constitutive nature of Japanese upland rice,Oryza sativa L." Eupytica. 98. 197-203 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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