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Genetic transformation and various biotechnological treatments on hairy roots

Research Project

Project/Area Number 08660003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionChiba University

Principal Investigator

MII Masahiro  Faculty of Horticulture, Chiba University, Professor, 園芸部, 教授 (30093074)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGenetic engineering / Genetic transformation / Agrobacterium / Hairy roots / GUS gene / Mitotic synchronization / Polyploid production / Colchicine / 細胞分裂の同 / 調化 / 遺伝子導入 / Agrobacterium / 細胞分裂同調化 / 染色体倍加 / 倍数体 / tissue electroporation
Research Abstract

In this study, transformation of several horticultural plants with Agrobacterium rhizogenes was tried and the hairy roots thus obtained were used as the targets for the studies on the genetic transformation and various biotechnological studies. The results obtaiend are as follows :
1. Plant regeneration systems have been established from the hairy roots obtained by infection of leaf segments with wild-type strains of Agrobacterium rhizogenes in Nierembergia repens, N.acoparia, Ipomoea trichocarpa, snapdragon and grape vine.
2. Root tips of N.scoparia were infected with A.tumefaciens harboring GUS and NPT II genes. Hairy roots expressing GUS activity and resistance to kanamycin were selected and transgenic plants were regenerated from these hairy roots.
3. Root tip meristems were subjected with various treatments for mitotic synchronization and the highest mitotic index (30%) was obtained with the combination of 3 treatments including phosphate starvation, hydroxyurea and colchicine treatments.
4. Trials for isolating chromosomes from the synchronized root tip cells resulted in failure due to the break down of the chromosomos during the isolation process and difficulty in purification of the chromosomes.
5. Tetraploid plants were successfully regenerated from the hairy root cultures after the treatment with colchicine by adjusting the proper time and concentration.
6. These results indicate that root cultures including hairy roots are useful materials for genetic transformation and other biotechnological treatments.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Otani,M.: "Fertile transgenic plants of Ipomoea trichocarpa Ell. induced by different strains of Agrobacterium rhizogenes." Plant Science. 116. 169-175 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Goto,T.: "Fertile transgenic plants of Nierembergia scoparia Sendtner obtained by a mikimopine type strain of Agrobacterium rhizogenes." Scientia Horticulturae. 68. 101-111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino,Y.: "Production of transgenic grape vine (Vitis vinifera L.cv.Koshusanjaku) plants by co-cultivation of embryogenic calli with Agrobacterium tumefaciens and selecting secondary embryos." Plant Biotechnology. 15. 29-33 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino,Y.: "Transgenic bialaphos-resistant snapdragon (Antirrhinum majus L.) produced by Agrobacterium rhizogenes." Scientia Horticulturae. 76. 37-57 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Otani et al.: "Fertile transgenic plants of Ipomoea trichocarpa Ell.Induced by differcnt strains of Agrobacterium rhizogenes." Plant Sciencce. 116. 169-175 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Godo et al.: "Fertile transgenic plants of Nierembergia scoparia Sendtner obtained by a mikimopine type strain of Agrobaeterium rhizogenes." Scientia Horticulturae. 68. 101-111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino et al.: "Production of transgenic grape vine (Vitis vinifera L.cv.Koshusanjaku) plants by co-cultivation of embryogenic calli with Agrobacterium tumefaciens and selecting secondary embryos." Plant Biotechnology. 15. 29-33 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino et al.: "Bialaphos stimulates shoot regeneration from hairy roots of snapdragon (Antirrhinum majus L.) transformed by Agrobacterium rhizogenes." Plant Cell Reports. 17. 256-261 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino et al.: "Transgenic bialaphos-resistant snapdragon (Antirrhinum majus L.) produced by Agrobacterium rhizogenes transformation." Scientia Horticulturae. 76. 37-57 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary

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Published: 1996-04-01   Modified: 2016-04-21  

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