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Nuclear encoded chloroplast ribosomal protein genes ; genomic structure and organ-specific gene expression.

Research Project

Project/Area Number 08660004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionThe University of Tokyo

Principal Investigator

TSUTSUMI Nobuhiro  Univ.Tokyo, Grad.Schl.Agr.Life Sci., Assoc. Prof., 大学院・農学生命科学研究科, 助教授 (00202185)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordschloroplast ribosomal protein / RFLP mapping / gene expressionon / rice / 転写開始点 / rpl13 / rpl24 / イントロン / S1F
Research Abstract

A cDNA library was constructed with poly(A)^+RNA prepared from 10-day-old rice seedlings. I have isolated three nuclear genes, rpl9, rpl12-1, rpl12-2, rpl13, rpl15, rp121, rpl24, rpl28, rps1 and rps9, that encode chloroplast ribosomal proteins L9, L12, L13, L15, L21, L24, L28, S1 and S9 respectively. Using these clones as probes, these nuclear encoded genes were located on molecular linkage map of rice. Seven loci were located on 5 differnt chromosomes of rice. They were not clustered but dispersed throughout the rice genome.
Expression analysis of rpl12-1, rpl12-2, rpl13, rpl24 by Northern blotting reveals that these genes were expessed in leaves of 10-day-old rice seedlings regardless of the presence of light, but not in roots. These data suggest that nuclear encoded ribosomal protein genes were expressed in an organ specific manner.
The genomic clones of rpl13 and rpl24 were isolated, and transcription start sites were determined by S1-nulcease mapping and primer extension. It is suggested that both genes have multiple sites of transcription initiation, and that each site was employed for transcription initiation differently in specific organ.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 堤 伸浩: "Molecular cloning and nucleotide sequencing of nuclear genes coding for the chloroplast ribosomal protein L13,L24,L28 of rice(Oryza sativa L.)." Plant Science. 15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤 裕介: "Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 日下 宗規: "Characterization of two rice genes for nuclear-encoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor.Appl.Genet.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 金澤 章: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes Genet.Syst.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 有村 慎一: "Genomic structures and the 5´-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice(Oryza sativa L.)." Breed.Sci.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsutsumi N.: "Molecular cloning and nucleotide seqrencing of nuclear genes coding for the chloroplast ribosomal protein L13, L24, L28 of rice (Oryza sativa L.)" Plant Science. 15. 6652-6661 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh Y.: "Transcript levels of tendem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh Y.: "Transcript levels of tendem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)Kusaka M.:

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] "Characterization of two rice genes for nuclearencoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor. Appl. Genet. ( in press ).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kanezawa A.: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes & Genet. Syst.( in press ).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arimura S.: "Genomic structures and the 5'-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice ( Oryza sativa L.)" Breed. Sci.( in press ).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 伊藤裕介: "Transcript levels of tandem-arranged alternative oxidase genes in rice are increased by low temperature." Gene. 203. 121-129 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 日下宗規: "Characterization of two rice genes for nuclear-encoded chloroplast ribosomal protein L12 and phylogenetic analysis of the acquisition of transit peptides and gene duplication." Theor.Appl.Genet.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 金澤章: "Phylogenetic relationships in the genus Nelumbo based on polymorphism and quantitative variations in mitochondrial DNA." Genes Genet.Syst.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 有村慎一: "Genomic structures and the 5'-flanking regions of the nuclear genes for chloroplast ribosomal proteins L13 and L24 of rice(Oryza sativa L.)." Breed.Sci.(印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Nobuhiro Tsutsunm: "Molecular cloning and nucleotide sequencing of nuclear genes coding for the chloreplast ribosumal proteins L13,L24,L28 of rice (Opse salival)" Plant Science. 121. 167-174 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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