ANALYSIS OF THE MECHANISMS OF NITROGEN INHIBITION ON RHIZOBIA INFECTION ON PEANUT USING AGROBACTERIUM RHIZOGENES-MEDIATED TRANSGENIC HAIRY ROOTS
| Project/Area Number |
08660019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
作物学
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| Research Institution | OSAKA PREFECTURE UNIVERSITY |
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Principal Investigator |
DAIMON Hiroyuki OSAKA PREFECTURE UNIVERSITY COLLEGE OF AGRICULTURE ASSOCIATE PROFESSOR, 農学部, 助教授 (50236783)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Agrobacterium / Hairv root / Nitrate nitrogen / Nitrogen fixation / Peanut / Root nodule |
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| Research Abstract |
Agrobacterium rhizogenes-mediated transformed hairy roots were induced on dry mature seed of peanut (Arachis hypogaea) cv.Java 13. Composite plants consisting of transformed roots with non-transformed shoots showed a root system with abundant root mass, more lateral branching and high fractal dimension compared to the control plant. The inoculation of Bradyrhizobium sp.to the composite plants led to the induction of transformed root nodules. Those transformed root nodules showed production of leghemoglobin in the bacterial zone and nitrogenase activity by C_2H_2 reduction assay, and exhibited morphological alterations such as enlargement of nodule cortex region and de novo root formation from nodule cortex.
The effect of nitrate nitrogen at levels of 300 ppm N was investigated on the growth, root nodule formation and nitrogenase activity in peanut (cv.Chiba-handachi) inoculated with Bradyrhizobium and cultured in solution medium. Dry weight and total nitrogen content of the plant differed in response to the duration of supplement of nitrogen to the culture solution, i.e.supplement at early growing stage increased nodule numbers, nodule fresh weight and acetylene reduction activity. Pegging and pod formation were found on this hydroponic culture system.
Further investigation of nitrate inhibition mechanisms on rhizobia infection on peanut is now in progress using the composite plant and this culture method.
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Report
(3 results)
Research Products
(17 results)
