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Fundamental study for citrus chromosome mapping

Research Project

Project/Area Number 08660034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 園芸・造園学
Research InstitutionKochi University

Principal Investigator

KITAJIMA Akira  Kochi Univ., Fac. of Agriculture Associate Professor, 農学部, 助教授 (70135549)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordscitrus / chromosome / karyotype snslysis / fluorescent stain
Research Abstract

Major purpose of this study is to develop new chromosome preparation method for citrus karyotype analysis that is important of gene chromosome mapping, and the second is to try citrus karyotype analysis using this new method and to develop useful chromosome banding method for identification of citrus chromosome.
1.Optimum enzymatic treatment was 120 min.incubation of 2% cellulase + 1% pectolyase or 90 min.incubation of 3% cellurase + 2% pectolyase for root tip of in vitro cultured plantlet, and 90min.incubation of 3% cellurase + 1% pectolyase for young leaf tip from orchard tree. The chromosome length of the longest one in each chromosome setreached 4-10mum that was over 2-5 times of the longest chromosome length which had been reported.
2.The chromosome numbers of 2n=18 in Poncirus and orange, 2n=20-21 in 'Tosa-Buntan' and 'Suisho-Buntan', 2n=19 in satsuma mandarin, 2n=20 in Fortunella and 2n=19-20 in yuzu were major in this investigation. The karyotyape analysis of Poncirus and orange indicated that both had 2 pairs of metacentric and 7 pairs of submetacentric chromosome, and 6 paires of Poncirus chromosome and 5 pairs of orange chromosome were possible to be identified.
3.G banding and N banding method was not available for citrus chromosome. C banding method showed unique banding pattern but repeatability was very low in the present study. CMA/DAPI stain showed stable unique band in 'Tosa-Buntan' chromosome. There were 3 types of chromosome that having 2 bands, 1band and no band.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 北島 宣: "カンキツ類の染色体標本作成における酵素処理条件の検討と染色体数" 園芸学会雑誌. 66(別2). 190-191 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kitajima, A.: "Optimum enzyme treatment for chromosome preparation method and chromosome number in Citrus and Poncirus." J.Japan.Soc.Hort.Sci.66 (Suppl.2). 190-191 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 北島 宣: "カンキツ類の染色体標本作成における酸素処理条件の検討と染色体数" 園芸学会雑誌. 66(別2). 190-191 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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