| Project/Area Number |
08660034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | Kochi University |
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Principal Investigator |
KITAJIMA Akira Kochi Univ., Fac. of Agriculture Associate Professor, 農学部, 助教授 (70135549)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | citrus / chromosome / karyotype snslysis / fluorescent stain |
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| Research Abstract |
Major purpose of this study is to develop new chromosome preparation method for citrus karyotype analysis that is important of gene chromosome mapping, and the second is to try citrus karyotype analysis using this new method and to develop useful chromosome banding method for identification of citrus chromosome.
1.Optimum enzymatic treatment was 120 min.incubation of 2% cellulase + 1% pectolyase or 90 min.incubation of 3% cellurase + 2% pectolyase for root tip of in vitro cultured plantlet, and 90min.incubation of 3% cellurase + 1% pectolyase for young leaf tip from orchard tree. The chromosome length of the longest one in each chromosome setreached 4-10mum that was over 2-5 times of the longest chromosome length which had been reported.
2.The chromosome numbers of 2n=18 in Poncirus and orange, 2n=20-21 in 'Tosa-Buntan' and 'Suisho-Buntan', 2n=19 in satsuma mandarin, 2n=20 in Fortunella and 2n=19-20 in yuzu were major in this investigation. The karyotyape analysis of Poncirus and orange indicated that both had 2 pairs of metacentric and 7 pairs of submetacentric chromosome, and 6 paires of Poncirus chromosome and 5 pairs of orange chromosome were possible to be identified.
3.G banding and N banding method was not available for citrus chromosome. C banding method showed unique banding pattern but repeatability was very low in the present study. CMA/DAPI stain showed stable unique band in 'Tosa-Buntan' chromosome. There were 3 types of chromosome that having 2 bands, 1band and no band.
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