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STUDIES ON THE TRANSCRIPTION FACTOR VP1 INVOLVED IN ABSCISIC ACID RESPOSIVE GENE EXPRESSION

Research Project

Project/Area Number 08660105
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionMIE UNIVERSITY

Principal Investigator

HATTORI Tsukaho  MIE UNIVERSITY,CENTER FOR MOLECULAR BIOLOGY AND GENETICS,ASSOCIATE PROFESSOR, 遺伝子実験施設, 助教授 (10164865)

Co-Investigator(Kenkyū-buntansha) TOYODA Akiko  MIE UNIVERSITY,CENTER FOR MOLECULAR BIOLOGY AND GENETICS,ASSISTANT PROFESSOR, 遺伝子実験施設, 助手 (70263027)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsABA / abscisic acid / rice / VP1 / transcription factor / OSVP1 / VIP-C1 / two-hybrid / Twn-hybrid / GFP / アラビドプシス
Research Abstract

G-box-like response elements (ABREs) have been functionally identified in the promoters of various genes. In addition, single copies of ABRE have been found to require a cis-acting, coupling element to achieve ABA induction. A Coupling Element 3 (CE3) sequence, originally identified as such in the barley HVA1 promoter, is found approximately 30-bp downstream of motif A (G-box-like ABRE) in the promoter of Osem gene. The relationship between these two elements was further defined by linker-scan analyzes of a 55-bp fragment of the Osem promoter, which is sufficient for ABA-responsiveness and VP1 activation. The analyzes revealed that both motif A and CE3 sequence were required not only for ABA-responsiveness but also for VP1 activation. Since the sequences of motif A and CE3 were found to be similar, motif-exchange experiments were carried out. The experiments demonstrated that motif A and CE3 were interchangeable by each other with respect to both ABA and VP1 regulation. Furthermore, both sequences were shown to be recognized by a rice bZIP factor OSBZ8. These results indicate that G-box-like ABREs and CE3 are functionally equivalent cis-acting elements.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Carson, C.B.: "The quiescent/colorless alleles of viviparous/ show that the conserved B3 domain of VP1 is not essential for ABA-regulated expression in the seed." Plant J.12. 1231-1240 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Carson, C.B.Hattori, T., Rosenkrans, L., Vasil, V., Vasil, I.K., PetersonP.P.and McCarty, D.R.: "The quiescent/colorless allele of viviparous1 show that the conservedB3 domain of VP1 is not essential for ABA-regulated gene expression in the seed." Plant J.12. 1231-1240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Carson,C.B.: "The guiescent/colorless alleles of viviparousl show That the conserved B3domain of VP1 is not essential for ABA-regulated expression in the seed." Plant J.12. 1231-1240 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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