| Project/Area Number |
08660105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | MIE UNIVERSITY |
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Principal Investigator |
HATTORI Tsukaho MIE UNIVERSITY,CENTER FOR MOLECULAR BIOLOGY AND GENETICS,ASSOCIATE PROFESSOR, 遺伝子実験施設, 助教授 (10164865)
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| Co-Investigator(Kenkyū-buntansha) |
TOYODA Akiko MIE UNIVERSITY,CENTER FOR MOLECULAR BIOLOGY AND GENETICS,ASSISTANT PROFESSOR, 遺伝子実験施設, 助手 (70263027)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | ABA / abscisic acid / rice / VP1 / transcription factor / OSVP1 / VIP-C1 / two-hybrid / Twn-hybrid / GFP / アラビドプシス |
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| Research Abstract |
G-box-like response elements (ABREs) have been functionally identified in the promoters of various genes. In addition, single copies of ABRE have been found to require a cis-acting, coupling element to achieve ABA induction. A Coupling Element 3 (CE3) sequence, originally identified as such in the barley HVA1 promoter, is found approximately 30-bp downstream of motif A (G-box-like ABRE) in the promoter of Osem gene. The relationship between these two elements was further defined by linker-scan analyzes of a 55-bp fragment of the Osem promoter, which is sufficient for ABA-responsiveness and VP1 activation. The analyzes revealed that both motif A and CE3 sequence were required not only for ABA-responsiveness but also for VP1 activation. Since the sequences of motif A and CE3 were found to be similar, motif-exchange experiments were carried out. The experiments demonstrated that motif A and CE3 were interchangeable by each other with respect to both ABA and VP1 regulation. Furthermore, both sequences were shown to be recognized by a rice bZIP factor OSBZ8. These results indicate that G-box-like ABREs and CE3 are functionally equivalent cis-acting elements.
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