Pure Isolation and Applications of High-Temperature-Resistant Nitrifying Bacteria

• Project/Area Number: 08660122
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 応用微生物学・応用生物化学
• Research Institution: College of Bioresource Sciences, Nihon University
• Principal Investigator: TOKUYAMA Tatsuaki Dept.of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University, Professor, 生物資源科学部, 教授 (90059684)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Reiji Dept.of Agricultural and Biological Chemistry, College of Bioresource Sciences,, 生物資源科学部, 講師 (70197193)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥2,300,000 (Direct Cost: ¥2,300,000); Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: Nitrifying bacteria / Ammonia-oxidizing bacteria / Pure isolation / High-temperature-resistant bacteria / 独立栄養細菌 / 亜硝酸酸化菌 / 高温性菌 / Nitrosomonas / Nitrobacter

Research Abstract

A high-temperature-resistant nitrifying bacteria, ammonia-oxidizing bacterium, strain K1, was newly isolated from packed tower biological deodorization plants of chicken farms. The cells of strain K1 are rods, gram negative, obligately aerobic, and nonmotile. Colonies (1-2 mm in diameter) on a plate culture are reddish, circular, and smooth, Intracytoplasmic membranes characteristic of nitrifying bacteria are present. The G+C content of the total DNA is 48.5 mol%. The similarity of 16S rRNA (%) to N.europaea ATCC 25978 T (type strain) is 93.77%. This bacterium has a higher optimal growth temperature (35ﾟC) than is usually the case and tolerance up to 40ﾟC.The optimum **ncentration of ammonium sulfate in the medium is 303 mM, which should make it applicable for use in deodorization plants for enhancing the efficiency of deodorization. Phosphoglycerate kinase (PGK) and triose phosphate isomerase (TIM) were found to possess high specific activities (5700 and 4*105 U/mg, respectively) compared to the activities of these enzymes in strain ATCC 25978T (300 and 14 U/mg). PGK was purified as electrophoretically homogeneous proteins from strain K1. A new marine ammonia-oxidizing bacterium (strain TNO632) was isolated in a screening of 80 sea-water samples on gellan gum-solidified plates. The optimum temperature for maximum growth in medium is 27-29ﾟC.

Research Products

• [Publications] Hatayama,R.,Chiba,K.,Noda,K.,Takahashi,R.,Kanehira,T.,Serata,K.,Shinahara,M.,and Tokuyama T: "Characteristics of high-concentration-ammonium sulfate-requiring ammonia-oxidizing bacterium isolated from deodorization plant at chicken farms." Journal of Bioscience and Bioengineering. 87(2)(in press). (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno,Y.,Ohshima,M.,Yao,Y.,Shibasaki,R.,Takahashi,R.,and Tokuyama,T: "Purification and comparisons of phosphoglycerate kinase from nitrifying bacteria" Journal of Fermentation and Bioengineering.86(No.4). 346-350 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizoguchi,M.,Omotani,J.,Mizuno,Y.,Takahashi,R.,Kanehira,T.,Shinohara,M.,and Tokuyama,T.: "Newly isolated marine ammonia-oxidizing bacterium,Nitrosomonas sp.TNO632" Journal of fermentation and bioengineering.86(No.4). 406-409 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hatayama, R., Chiba, K., Noda, K., Takahashi, R., Kanehira, T., Serata, K., Shinahara, M., and Tokuyama, T: "Characteristics of high-concentration-ammonium sulfate-requiring ammonia-oxidizing bacterium isolated from deodorization plant at chicken farms" Journal of Bioscience and Bioengineering. 87(2) (in press). (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuno, Y., Ohshima, M., Yao, Y., Shibasaki, R., Takahashi, R., and Tokuyama, T: "Purification and comparisons of phosphoglycerate kinase from nitrifying bacteria" Journal of Fermentation and Bioengineering. 86(No.4). 346-350 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuguchi, M., Omotani, J., Mizuno Y., Takahashi, R., Kanehira, T., Shinohara, M., and Tokuyama, T.: "Newly isolated marine ammonia-oxidizing bacterium, Nitrosomonas sp.TNO632" Journal of fermentation and bioengineering. 86(No.4). 406-409 (1998)
  • Description: 「研究成果報告書概要(欧文)」より