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Study on the flower color change by measuring vacuolar pH

Research Project

Project/Area Number 08660141
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionSugiyama Jogakuen University

Principal Investigator

YOSHIDA Kumi  Shool of Life Studies, Sugiyama Jogakuen University Assistant professor, 生活科学部, 助手 (90210690)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsIpomoea tricolor / vacuolar pH / pH sensitive microelectrode / protoplast / V-ATPase / floser color change / vacuolar membrane / PPase / 細胞内微少pH電極 / 西洋アサガオ / プロトプスト / プロトンポンプ
Research Abstract

The petal of blue morning glory, Ipomoea tricolor cv. heavenly blue, changes its color from purplish red to light blue during blooming. The pigment, heavenly blue anthocyanin, was distributed in the vacuoles of upper and lower epidermis and not in parenchyma. We measured pHv of living petal cell directly using proton selective-microelectrode. The pHv of blue open flower was 7.7 while that of purplish red buds was 6.7. This study provides the clear evidence that the color change is caused by the increase of pHv during blooming.
To clarify the pH increasing mechanism the colored protoplasts were prepared from the petals of purplish-red buds, blue open flower and purple faded flower, followed by the vacuolar membrane were provided. By immunoblot technique H^+-ATPase and H^+-PPase retained both in the buds and the open flower petals. In contrast those proteins were absent in faded petals. PPi-and ATP-dependent H^+-transport activities in vacuolar membrane vesicles from the buds and the open flower petals were almost in the same level, although no activities in those from the faded flower petals. Therefore the vacuolar pH was not controlled by proton pumps, but might be concerning with another ion transport.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Yoshida, K.et al: "Structural analysis and measurement of anthocyanins from colored seed coats of Vigna,Phaseolus,and glycine legumes." Biosci.Biotech.Biochem.60. 589-593 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 吉田 久美、近藤 忠雄: "細胞内微小電極法による花色変異の解明-アサガオの花はなぜ青色になるのか?" 電気化学および工業物理化学. 64. 1147-1152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K.Kameda, K., Kondo, T.: "PERILLA The Genus Perilla" Ed.Yu,H.,Kosuna,K.,Haga,M.,harwood academic publishers, 189(22) (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K., Sato, Y., Okuno, R., Kameda, K., Isobe, M., Kondo, T.: "Structural analysis and measurement of anthocyanins from colored seed coats of Vigna, Phaseolus, and Glycine legumes." Biosci.Biotech.Biochem.60. 589-593 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K., Kondo, T.: "Study on the mechanism of flower color variation by using proton selective micro-electrode-Why the petal of morning glory turns blue? -" Denki Kagaku. 64. 1147-1152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K., Kameda, K.Kondo, T.: The Chemistry and Applications of Anthocyanins and Flavones from Perilla Leaves. in Perilla. (He-ci, Y., Kosuna, K.and Haga, M.eds.) Harwood Academic Publishers, Amsterdam, 149-169 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida,K.et al.: "PERILLA The Genus Perilla" Ed.Yu,H.,Kosuna,K.,Haga,M.,harwood academic publishers, 189(22) (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Yoshida et al.: "Structural Analysis and Meaxurement of Anthocyanins from Colored Seed Coats of Vigna,Phaseolus,and Glycine Legumes" Biosci.Biotech Biochem.60(4). 589-593 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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