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ADVENTITIOUS EMBRYO FORMATION BY SYNCHRONOUS CULTURE FROM MATUR EMBRYO IN CONIFEROUS SPECIES

Research Project

Project/Area Number 08660200
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

KUDO Takafumi  Tokyo University of Agriculture and Technology, Faculty of Agriculture, Professor, 農学部, 教授 (00015091)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordskaramatsu (Larix leptolepis) / ezomatsu (Picea jezoensis) / immature embryo / mature embryo / embryogenic callus / adventitious embryo / colony / suspension culture / 縣濁培養 / 窒素源 / NH_4NO_3 / L-gulutamine
Research Abstract

1) Induction of adventitious embryo from callus of embryo in karamatsu (Larix leptolepis) and ezomatsu (Picea jezoensis)
(1) Induction of adventitious embryo from callus of immature and mature embryo in karamatsu
White and friable embryogenic callus were derived from immature embryo by culture in CD medium with 7 mu M of 2,4-D and 3 mu M of BAP for 12 weeks. Red callus, obtained by subculturing the induction callus over 1 year, induced the early adventitious embryo, resulting in the germination, in liquid medium containing 1 mu M of ABA and 0.3 mu M of BAP.
From callus of mature embryo, embryogenic tissues were formed in liquid medium, which was and identical component to the solid, but the rate of formation was very low.
(2) Induction of adventitious embyo from callus of mature embryo in ezomatsu
In mature embryo of ezomatsu, white friable embryogenic callus were frequently induced by culture in LP medium with 10 mu M of 2,4-D and 5 mu M of BAP for 12 weeks.
2) An attempt for adventitious embryo formation by suspension culture from callus of immature embryo in karamatsu (Larix leptolepis)
Dissociated callus, regulated in size of 300 mu m to 30 mu m by meshes, was diluted to two cell densites, as 5.0*104 cells/iota and 1.2*104 cells/iota, and colony was induced in liquid CD medium with 7 mu M of 2,4-D and 3 mu M of BAP.However, as abilities of the propagation and the differentiation of colony begun to decrease after culturing for 14 days, the colony, then, was subcultured in medium containing high cytokinin and low auxin. Consequently, colony with high ability of the propagation and the differentiation was formed in cell density condition of 5.0*104 cells/iota.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ogita, S., kubo, T.,: "Caulogenic callus induction and adventitious bud formation from embryos of long-term stored seeds of Picea jezoensis" Journal of Forest Research. 2(3). 141-145 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita, S., kubo, T.: "Anatomical characteristics in early embryogenesis from immature embryo of Larix leptolepis" Forest Resources Environment. 35. 45-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita, S., kubo, T.: "Histological and characteristicsrelated to adventitious bud formation from calli of mature embryos in Larix leptolepis" Mokuzai Gakkaishi. 42(11). 1042-1048 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita, S., kubo, T., Fushitani, M.: "Caulogenic callus induction and adventitious bud formation from embryos of long-term stored seeds of Picea jezoensis" Journal of Forest Research. 2(3). 141-145 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita, S., kubo, T., Fushitani, M.: "Anatomical characteristics in early embryogenesis from immature embryo of Larix" Forest Resources Environment. 35. 45-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita, S., kubo, T., Fushitani, M.: "Histological and characteristics related to adventitious bud formation from calli of mature embryos in Larix leptolepis" Mokuzai Gakkaishi. 42(11). 1042-1048 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ogita,S., Kubo,T.: "Histological and Morphological Characteristies Related to Adventitious Bud." Mokuzai Gakkaishi. 42・11. 1042-1048 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ogita,S., Kubo,T.: "Caulogenic Callus Induction and Adventitious Bud Formation from Embryos" Journal of Forest Research. 2・3. 141-145 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ogita,S., Kubo,T.: "Anatomiceal Charactevistics in Eary Embryogenesis from Immature Embryo" Forest Researchs Environment. 35. 45-51 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ogita,S.,Kubo,T.: "Histological and Morphological Characteristics Related to Adventitions Bud Formation" Mokuzai Gakkaishi. 42・11. 1042-1048 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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