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Study on endogenous gibberellins in trees and utilization of gibberellins produced by microbial conversion of kaurene

Research Project

Project/Area Number 08660208
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林産学
Research InstitutionEhime University

Principal Investigator

TACHIBANA Sanro  Ehime University, Faculty of Agriculture, Professor, 農学部, 教授 (10112319)

Co-Investigator(Kenkyū-buntansha) ITOH Kazutaka  Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50253323)
NINOMIYAI Ikuo  Ehime University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80172732)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsGibberellins / Microbial conversion / Gibberella fujikuroi / kaurene / Anti-tumor agent / Elongation of sprouts / Trees / Protoplant fusion / Gibberella fujikuroi菌 / イヌガヤ / 融合菌 / キャラボク
Research Abstract

Gibberellins in sprouts of Kyaraboku (Taxus cuspidata var.nana), formation of fusants by protoplasts fusion of Gibberella fujikuroi, and the effect of additon of gibberellins produced by a microbial conversion with G.fujikuroi on elongation in Kyaraboku and Inugaya (Cephalotaxus harringtonia) sprouts were examined.
1. Gibberellin-A3 (GA3), GA4, and a glucosylester of gibberellin were isolated from Kyaraboku sprouts.
2. Three fusants (F1, F2, and F3) were formed by protoplasts fusion between G.fujikuroi IFO 9977 and IFO 30337. Each fusant produced GA3, iso-GA3, GA4, GA7, and iso-GA7 by the microbial conversion of (-) -kaurene, as parents fungi produced the same gibberellins by the microbial conversion. However, amounts of production of gibberellins by F2 and F3 fusants were about 1.5 times larger than those of parents fungi.
3. Kyaraboku and Inugaya sprouts were elongated about 1.2 times and about 1.1 times longer than those of the control (no addition of the gibberellins produced by the microbial conversion), respectively, when the gibberellins were added to Kyaraboku and Inugaya stems. Furthermore, the content of taxol, a strong anti-tumor agent, in Kyaraboku sprouts was increased about 1.1 times larger than that of the control (no addition of the gibberellins). The contents of harringtonine aklaloids (an anti-tumor agent), especially harringtonine and isoharringtonine, in Inugaya sprouts were increased about 1.2 times larger than that of the control (no addition of the gibberellins)

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] T.Hirabayashi: "Screening of a Strain of Gibberella fujikuroi gor Improved Microbial Conversion of (-)-kaurene" Mokuzai Gakkaishi. 40巻・11号. 1134-1138 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柳田 高志: "Gibberella fujikuroi菌のプロトプラスト融合による融合菌作出の試み" 愛媛大学農学部演習林報告. 第34号. 93-106 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柳田 高志: "キャラボク中のジベレリンおよび微生物変換によるジベレリン生産能の高い細胞融合菌作出の試み" 第46回日本木材学会大会研究発表要旨集. 419-419 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柳田 高志: "カウレンの微生物変換により生成したジベレリンの有効利用(1)" 第47回日本木材学会大会研究発表要旨集. 411-411 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tatsuya Hirabayashi et al.: "Screening of a Strain of Gibberella fujikuroi for Improved Microbial Conversion of (-) -kaurene." Mokuzai Gakkaishi. 40 (11). 1134-1138 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takashi Yanagida et al.: "Formation of fusants by protoplast fusion between Gibberella fujikuroi." Bull.Ehime Forest Research. No.34. 93-106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takashi Yanagida et al.: "Formation of fusants having higher productivity of gibberellins produced by a microbial conversion of (-) -kaurene and gibberellins in Kyaraboku sprouts" ABSTRACTS OF THE 40th ANNIVERSARY CONFERENCE OF THE JAPAN WOOD RESEARCH SOCIETY,kumamoto. 419 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takashi Yanagida et al.: "Utilization of gibberellins produced by a microbial conversion of (-) -kaurene with gibberella fujikuroi (1)." ABSTRACTS OF THE 40th ANNIVERSARY CONFERENCE OF THE JAPAN WOOD RESEARCH SOCIETY,kouchi. 411 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 柳田高志: "カウレンの微生物変換により生成したジベレリンの有効利用(1)" 第47回日本木材学会大会研究発表要旨集. 411-411 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 平林達也ら: "Screening of a Scrain of Gibberella fujikuroi for Improved Microbial Conversion of (-)-kaurine" Mokuzai Gakkaishi. 40巻11号. 1134-1138 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 柳田高志: "キャラボク中のジベレリンおよび微生物変換によるジベレリン生産能の高い細胞融合菌作出の試み" 第46回日本木材学会大会研究発表要旨集. 419-419 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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