| Project/Area Number |
08660209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | Miyazaki University |
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Principal Investigator |
MEGURO Sadatoshi Miyazaki univ. ; Agriculture ; Assoc.Prof., 農学部, 助教授 (50112321)
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| Co-Investigator(Kenkyū-buntansha) |
KAWACHI Shinsaku Miyazaki univ. ; Agriculture ; Prof., 農学部, 教授 (00040858)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Lentinula edodes / Thiamin / Fruit body formation / Mycelial growth / Liquid media / Thiamin uptake / 子実体誘導機構 / ペプトン・グルコース液体培地 / チアミン二リン酸エステル |
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| Research Abstract |
The effects of thiamin on vegetative mycelial growth and fruit body formation of Lentinula edodes were investigated in basal peptone-glucose liquid media in relation to the uptake of thiamin. Thiamin is essential for fruit body formation, and the minimum requirements for thiamin are estimated to be approximately 10 mug/L.The vegetative mycelial growth was little influenced by the addition of thiamin in the range of 0-1.5mg/L.While the mycelia was successively transferred to fresh peptone-glucose-agar media. three times, the repression of mycelial growth was not significant. Even in cases using vitamin-free casamino acid or glutamic acid as a nitrogen source instead of peptone, a thiamin deficiency for mycelial growth did not occur as a result of transferring the mycelia to fresh media. Almost of all the thiamin contained in the media was taken and accumulated in the mycelia during the first 3 weeks of a 9-week incubation. These results suggested that only trace amounts of thiamin are required for the vegetative mycelial growth of Lentinula edodes. Because Lentinula edodes requires approximately 10 mug/L thiamin for fruiting, all the thiamin added to the basal media must be used for fruit body formation.
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