• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunosuppresive action of milk k-casein and its digests

Research Project

Project/Area Number 08660328
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionShinshu University

Principal Investigator

OTANI Hajime  Shinshu University, Faculty of Agriculture, Professor, 農学部, 教授 (30109201)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordskappa-casein / milk proteins / lymphocytes / immunoglobulin production / mitogen / apoptosis / human casein / 牛乳 / 人乳 / 免疫抑制作用 / グリコマクロペプチド / IL-2レセプター / IL-1レセプターアンタゴニスト
Research Abstract

The results obtained for 2 years (1996 and 1997) are summarized as follows :
1. Bovine kappa-casein and it glycomacropeptide (residues 106-169, CGP) completely inhibited mouse T-lymphocyte proliferation via at least 2 different mechanisms : production of interleukin-1 receptor antagonist and suppression of interleukin-2 receptor expression on CD4+ T-lymphocytes.
2. Reverse transcription-polymerase chain reaction analysis showed that macrophages transcribed interleukin-1 receptor antagonist mRNA in both the absence and the presence of CGP.CGP increased interleukin-1 receptor antagonist biosynthesis and/or secretion without influencing secretion level of interleukin-1 alpha and interleukin-1 beta.
3. Bovine kappa-casein significantly inhibited macrophage activation and phagocytosis of macrophages. Both inhibitory activities was little influenced by trypsin or chymotrypsin digestion while pepsin digestion changed the inhibitory activity into an enhancing activity.
4. Orally ingestion of bovine CGP suppressed the level of serum IgG antibodies specific to dietary protein and intraperitoneally injected protein in mice. Mitogenic responses of spleen cells indicated that the suppressor T-lymphocyte number and/or function were significantly increased in mice fed the CGP-added diet.
5. Human CGP revealed a cytotoxic activity when the peptide was incubated with mouse lymphocytes. In addition, the chromatin of lymphocytes incubated with human CGP coagulated and DNAs extracted from the cells segmented. These results suggested that human CGP had an apoptosis-inducing ability.
Cytotoxic activity was also produced when bovine CGP was digested with trypsin.
These results suggest that kappa-casein and its digest may contribute to anti-inflammatory response in neonatal intestine. In addition, CGP prepared from cheese whey is available as an ingredient to prepare immunosuppressive foods

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] H.Otani et al.: "Suppression of interleukin-2 reoeptor expression on mouse CD_4^+ T cells by bovine κ-caseinoglycopeptide" Bioscience,Biotechnology,and Biochemistry. 60(6). 1017-1019 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Induction of interleukin-1 reoeptor antagonist produced from a mouse monocyte/macrophage cell hine,P388D1" Proceedings of the 8^<th> AAAP Animal Science Congress. 2. 1014-1015 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani & M.Futakami.: "Modification of nitrite production and phagocytosis of thioglycollate-elicited mouse peritoneal macrophages by bovine casein digests" Food & Agricultural Immunology. 8(1). 59-69 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Effect of bovine κ-ceseinoglycopeptide on secretion of interleukin-1 family cytokines by P388D1,a line derived from mouse monocyte/macrophages" Milchwissenschaft. 52(4). 192-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani: "Modification of immune system by peptides derived from milk caseins" Recent Research Developments in Agricultural & Biological Chemistry. 1. 269-289 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Immunomodificatory effect of dietary bovine κ-caseinoglycopeptide on serum antibody levels and proliferative responses of lymphocytes in mice" Milchwissenschaft. 53(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大谷 元(ミルクの機能研究会編): "「ミルクの先端機能」の「第I章、2節ミルクの栄養」と「第III章、1節ミルクの生理機能と効用」" 弘学出版(出版予定), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大谷 元(鈴木 敦士・渡部 終五・中川 弘毅 編): "「タンパク質の科学」の「第2章、3節乳」" 朝倉書店(出版予定), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani, Y.Horimoto & M.Monnai: "Suppression of interleukin-2 receptor expression on mouse CD4^+T cells by bovine kappa-caseinoglycopeptide" Bioscience, Biotechnology, and Biochemistry. 60 (6). 1017-1019 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Induction of interleukin-1 receptor antagonist produced from a mouse monocyte/macrophage cell line, P388D1" Proceedings of the 8^<th> AAAP Animal Science Congress. 2. 1014-1015 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani & M.Futakami: "Modification of nitrite production and phagocytosis of thioglycollate-elicited mouse peritoneal macrophages by bovine casein digests" Food & Agricultural Immunology. 8 (1). 59-69 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Effect of bovine kappa-caseinoglycopeptide on secretion of interleukin-1 family cytokines by P388D1, a line derived from mouse monocyte/macrophages" Milchwissenshaft. 52 (4). 192-196 (1977)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani: "Modification of immune system by peptides derived from milk caseins" Recent Research Developments in Agricultural & Biological Chemistry. 1. 269-289 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Monnai & H.Otani: "Immunomodificatory effect of dietary bovine kappa-caseinoglycopeptide on serum antibody levels and proliferative responses of lymphocytes in mice" Milchwissenshaft. 53 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Otani st al.: "Suppression of interleukin-2 reoeptor expression on mouse CD4^+T oells by bovine κ-caseinoglycopeptide" Bioscience,Biotechnology,and Biochemistry. 60(6). 1017-1019 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Monnai & H.Otani: "Induction of interleukin-1 receptor antagonist produced from a mouse monocyte/macrophage cell line,P388D1" Proceedings of the 8^<th>AAAP Animal Science Congress. 2. 1014-1015 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Otani & M.Futakami: "Modifcation of nitrite production and phagocytosis of thioglycollate-elicited mouse peritoneal macrophages by bovine casein digests" Food & Agricultural Immunology. 8(1). 59-69 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Monnai & H.Otani: "Effect of bovine κ-caseinoglycopeptide on secretion of interleukin-1 family cytokines by P388D1,a line derived from mouse monocyte/macrophages" Milchwiseenschaft. 52(4). 192-196 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Otani: "Modifcation of immune system by peptides derived from milk caseins" Recent Research Developments in Agricultural & Biological Chemistry. 1. 269-289 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Monnai & H.Otani: "Immunomodifcatory effect of dietary bovine κ-caseinoglycopeptide on serum antibody levels and proliferative responses of lymphocytes in mice" Milchwissenschalft. 53(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大谷 元(ミルクの機能研究会編): "「ミルクの先端機能」の「第1章、2節 ミルクの栄養」と「第III章、1節 ミルクの生理機能と効用」" 弘学出版(出版予定 印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大谷 元(鈴木敦士・渡部終五・中川弘穀編): "「タンパク質の科学」の「第2章、3節 乳」" 朝倉書店(出版予定 印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Otani,Y.Horimoto & M.Monnai: "Suppression of interleukin-2 rceptor expression on mouse CD4+T cells by κ-caseinoglycopeptide" Biosci.Biotech.Biochem.60. 1017-1019 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Monnai & H.Otani: "Induction of interleukin-1 receptor antagonist produced from a mouse monocyte/macrophage cell line, P388D1" Proceedings of the 8^<th> AAAP Animal Science Congress. 2. 1014-1015 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Otani & M.Futakami: "Modification of nitrite production of thioglycolate-elicited mouse peritoneal macrophages by bovine casein digests" Food & Agricultural Immunology. 8. 59-69 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Monnai & H.Otani: "Effect of bovine κ-caseinoglycopeptide on secretion of interleukin-1 family cytokines by P388D1 cells, a line derived from mouse monocyte/macrophages" Milchwissenschaft. 52(in press). (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi