| Project/Area Number |
08660343
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MATSUI Tohru Kyoto University Graduate School of Agriculture, Associate Professor, 農学研究科, 助教授 (40181680)
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| Co-Investigator(Kenkyū-buntansha) |
HIGUCHI Keiichi Kyoto University, Chest Diseases Research Institute, Lecturer, 胸部疾患研究所, 講師 (20173156)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Sheep / lnsulin like growth factor-I / lnsulin like growth factor-II / Protein deficiency / Cartilage / liver / タンパク質栄養 / IGF-I / IGF-II / 筋肉 / 脂肪 |
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| Research Abstract |
To study the mechanism of relationship between protein nutrition and bone growth, growing lambs were fed protein deficient diet or control diet and the gene expression of lnsulin like growth factor-I and lnsulin like growth factor-II were measured by RT-PCR in the growth cartilage. Additionally plasma concentrations of lnsulin like growth factor-I and lnsulin like growth factor-II were determined by dadio immunoassay. Male castrated lambs weighing approximately 20 kg were allotted to two groups in a double reversal design. These animals were fed control diet (crude protein content ; 17.5%) or protein deficient diet (crude protein content, 4.9%) . Energy contents in these diets satisified for its requirement. Protein deficiency suppressed body weight gain. Plasma insulin like growth factor-I concentration was lowered When lambs were fed the protein deficient diet. Compared to lnsulin like growth factor-I, plasma lnsulin like growth factor-II concentration was remarkably low in each group. And plasma lnsulin like growth factor-II was not affected by protein deficiency. Transcript of lnsulin like growth factor-I in the growth cartilage tended to be reduced by protein deficiency though this change was not significant. However, transcript of lnsulin like growth factor-II in the growth cartilage was not affected by protein deficiency. These results suggested that protein deficiency suppressed bone growth through reducing plasma lnsulin like growth factor-I concentration and locally produced lnsulin like growth factor-I in the growth cartilage. However, plasma lnsulin like growth factor-II and locally produced lnsulin like growth factor-II were not related to the retardation of bone growth in protein deficient sheep.
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