| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
Ganglioside receptor on the bovine erythrocytes was qpplied to the prevention and treatment of bovine theileriosis.
1. Production of monocional antibody against gangliosiode receptor, 1-active ganglioside (NeuGc), on the bovine erythrocytes for T.sergentl.
Monoclonal antibody against I-active ganglioside (NeuGc), SHS-21, of the IgM class was obtained by the immunization of BALB/c mice with bovine erythrocytes-derived I-active ganglioside (NeuGc) inserted into liposomes with Salmonella minnesota R595 lipopolysaccharides. Various purified gangliosides were used to determine the specificity of the monoclonal antibody. SHS-21 antibody was found to react with I-active ganglioside (NeuGc) and i-active ganglioside (NeuGc), but not with other gangliosides tested. It has been shown that I-active ganglioside (NeuGc) on the bovine erythrocytes surface was suggested to act as attachment side for T.sergenti merozoites. Thus, SHS-21 antibody is considered to be useful for the treatment of bovine theil
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eriosis.
2. Preparation of drug-laden liposomes containing I-active ganglioside (NeuGc).
To elucidate whether or not antiprotozoan agent-laden liposomes having I-active ganglioside (NeuGc) are able to prepare, experiment was performed. The result of the experiment was that I-active ganglioside (NeuGc) incorporated liposomes are able to encapsulate the antiprotozoan agent. This type of liposomes may lead to new approaches to the treatment of bovine theileriosis.
3. Application of liposomes containing I-active gangliosiode (NeuGc) for development of bovine theileriosis vaccines.
In order to evaluate the usefulness of liposomes containing I-active ganglioside (NeuGc) as oral vaccines for bovine theileriosis, the stability of liposomes and antibody responses to ganglioside antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1 : 1 : 2, molar ratio), distearoylphosphatidylchollne (DSPC) nd Chol (7 : 2, molar ratio), and DSPC,DPPS,and Chol (7 : 3 : 2 or 1 : 1 : 2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution. After the oral immunization of ganglioside antigen (ganglioside GM1) -containing liposomes composed of DPPC,DPPS,and Chol (1 : 1 : 2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. On the other hand, such liposomes were unable to induce any detectable anti-ganglioside GM1 IgG and IgM antibody responses by oral administration. In future, further studies will be necessary to develop the liposomes for the generation of serum IgG or IgM responses by oral immunization. Less
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